Identification and characterization of a second CD4-like gene in teleost fish

J・m Dijkstra, Tomonori Somamoto, Lindsey Moore, Ivar Hordvik, Mitsuru Ototake, Uwe Fischer

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

In fish, T cell subdivision is not well studied, although CD8 and CD4 homologues have been reported. This study describes a second teleost CD4-like gene, CD4-like 2 (CD4L-2). Two rainbow trout copies of this gene were found, -2a and -2b, encoding molecules sharing 81% aa identity. The 2a/2b duplication may be related to tetraploid ancestry of salmonid fishes. In the Fugu genome CD4L-2 lies head to tail with an earlier reported, very different CD4-like gene [Suetake, H., Araki, K., Suzuki, Y., 2004. Cloning, expression, and characterization of fugu CD4, the first ectothermic animal CD4. Immunogenetics 56, 368-374], which was designated CD4L-1 in the present article. The flanking genes of the Fugu CD4L-1 and CD4L-2 are reminiscent of the genes surrounding CD4 and LAG-3 in mammals. However, neither synteny nor phylogenetic analysis could decide between CD4 and LAG-3 identity for the fish CD4L genes. CD4L-1 and CD4L-2 share a tyrosine protein kinase p56lck binding motif in the cytoplasmic tail with CD4 but not with LAG-3. Trout CD4L-2 expression is highest in the thymus, similar to mammalian and chicken CD4, whereas Fugu CD4L-1 expression was highest in the spleen. However, CD4L-2 encodes only two IG-like domains, whereas CD4L-1, CD4 and LAG-3 encode four. The CD4-like genes 1 and 2 in fish apparently went through an evolution different from that of LAG-3 and CD4 in higher vertebrates.

Original languageEnglish
Pages (from-to)410-419
Number of pages10
JournalMolecular Immunology
Volume43
Issue number5
DOIs
Publication statusPublished - 01-02-2006

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Takifugu
Fishes
Genes
Synteny
Immunogenetics
Tetraploidy
Trout
Oncorhynchus mykiss
Protein-Tyrosine Kinases
Thymus Gland
Vertebrates
Tail
Organism Cloning
Mammals
Chickens
Spleen
Genome
T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Immunology
  • Molecular Biology

Cite this

Dijkstra, J・m ; Somamoto, Tomonori ; Moore, Lindsey ; Hordvik, Ivar ; Ototake, Mitsuru ; Fischer, Uwe. / Identification and characterization of a second CD4-like gene in teleost fish. In: Molecular Immunology. 2006 ; Vol. 43, No. 5. pp. 410-419.
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Dijkstra, J, Somamoto, T, Moore, L, Hordvik, I, Ototake, M & Fischer, U 2006, 'Identification and characterization of a second CD4-like gene in teleost fish', Molecular Immunology, vol. 43, no. 5, pp. 410-419. https://doi.org/10.1016/j.molimm.2005.03.005

Identification and characterization of a second CD4-like gene in teleost fish. / Dijkstra, J・m; Somamoto, Tomonori; Moore, Lindsey; Hordvik, Ivar; Ototake, Mitsuru; Fischer, Uwe.

In: Molecular Immunology, Vol. 43, No. 5, 01.02.2006, p. 410-419.

Research output: Contribution to journalArticle

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