Identification and characterization of PDGFR + mesenchymal progenitors in human skeletal muscle

A. Uezumi, S. Fukada, Naoki Yamamoto, M. Ikemoto-Uezumi, Masashi Nakatani, M. Morita, A. Yamaguchi, Harumoto Yamada, I. Nishino, Y. Hamada, Kunihiro Tsuchida

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Fatty and fibrous connective tissue formation is a hallmark of diseased skeletal muscle and deteriorates muscle function. We previously identified non-myogenic mesenchymal progenitors that contribute to adipogenesis and fibrogenesis in mouse skeletal muscle. In this study, we report the identification and characterization of a human counterpart to these progenitors. By using PDGFR as a specific marker, mesenchymal progenitors can be identified in the interstitium and isolated from human skeletal muscle. PDGFR + cells represent a cell population distinct from CD56 + myogenic cells, and adipogenic and fibrogenic potentials were highly enriched in the PDGFR + population. Activation of PDGFR stimulates proliferation of PDGFR + cells through PI3K-Akt and MEK2-MAPK signaling pathways, and aberrant accumulation of PDGFR + cells was conspicuous in muscles of patients with both genetic and non-genetic muscle diseases. Our results revealed the pathological relevance of PDGFR + mesenchymal progenitors to human muscle diseases and provide a basis for developing therapeutic strategy to treat muscle diseases.

Original languageEnglish
Article numbere1186
JournalCell Death and Disease
Volume5
Issue number4
DOIs
Publication statusPublished - 01-01-2014

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Skeletal Muscle
Muscles
Adipogenesis
Phosphatidylinositol 3-Kinases
Connective Tissue
Population
Cell Proliferation
Therapeutics

All Science Journal Classification (ASJC) codes

  • Immunology
  • Cellular and Molecular Neuroscience
  • Cell Biology
  • Cancer Research

Cite this

Uezumi, A. ; Fukada, S. ; Yamamoto, Naoki ; Ikemoto-Uezumi, M. ; Nakatani, Masashi ; Morita, M. ; Yamaguchi, A. ; Yamada, Harumoto ; Nishino, I. ; Hamada, Y. ; Tsuchida, Kunihiro. / Identification and characterization of PDGFR + mesenchymal progenitors in human skeletal muscle. In: Cell Death and Disease. 2014 ; Vol. 5, No. 4.
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Identification and characterization of PDGFR + mesenchymal progenitors in human skeletal muscle. / Uezumi, A.; Fukada, S.; Yamamoto, Naoki; Ikemoto-Uezumi, M.; Nakatani, Masashi; Morita, M.; Yamaguchi, A.; Yamada, Harumoto; Nishino, I.; Hamada, Y.; Tsuchida, Kunihiro.

In: Cell Death and Disease, Vol. 5, No. 4, e1186, 01.01.2014.

Research output: Contribution to journalArticle

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AU - Morita, M.

AU - Yamaguchi, A.

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