Identification and characterization of pseudorabies virus glycoprotein gM as a nonessential virion component

Johannes M. Dijkstra, Nico Visser, Thomas C. Mettenleiter, Barbara G. Klupp

Research output: Contribution to journalArticlepeer-review

65 Citations (Scopus)

Abstract

Sequence analysis within BamHI fragment 3 of the pseudorabies virus (PrV) genome revealed an open reading frame homologous to the UL10 gene of herpes simplex virus. A rabbit antiserum directed against a synthetic oligopeptide representing the carboxy-terminal 18 amino acids of the predicted UL10 product recognized a major 45-kDa protein in lysates of purified Pr virions. In addition, a second protein of 90 kDa which could represent a dimeric form was observed. Enzymatic deglycosylation showed that the PrV UL10 protein is N glycosylated. Therefore, it was designated PrV gM according to its homolog in herpes simplex virus. A PrV mutant lacking ca. 60% of UL10 coding sequences was able to productively replicate on non- complementing cells, demonstrating that PrV gM is not required for viral replication in cell culture. However, infectivity of the mutant virus was reduced and penetration was delayed, indicating a modulatory role of PrV gM in the initiation of infection.

Original languageEnglish
Pages (from-to)5684-5688
Number of pages5
JournalJournal of Virology
Volume70
Issue number8
DOIs
Publication statusPublished - 08-1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Fingerprint Dive into the research topics of 'Identification and characterization of pseudorabies virus glycoprotein gM as a nonessential virion component'. Together they form a unique fingerprint.

Cite this