Identification and characterization of pseudorabies virus glycoprotein gM as a nonessential virion component

Johannes M. Dijkstra, Nico Visser, Thomas C. Mettenleiter, Barbara G. Klupp

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Sequence analysis within BamHI fragment 3 of the pseudorabies virus (PrV) genome revealed an open reading frame homologous to the UL10 gene of herpes simplex virus. A rabbit antiserum directed against a synthetic oligopeptide representing the carboxy-terminal 18 amino acids of the predicted UL10 product recognized a major 45-kDa protein in lysates of purified Pr virions. In addition, a second protein of 90 kDa which could represent a dimeric form was observed. Enzymatic deglycosylation showed that the PrV UL10 protein is N glycosylated. Therefore, it was designated PrV gM according to its homolog in herpes simplex virus. A PrV mutant lacking ca. 60% of UL10 coding sequences was able to productively replicate on non- complementing cells, demonstrating that PrV gM is not required for viral replication in cell culture. However, infectivity of the mutant virus was reduced and penetration was delayed, indicating a modulatory role of PrV gM in the initiation of infection.

Original languageEnglish
Pages (from-to)5684-5688
Number of pages5
JournalJournal of Virology
Volume70
Issue number8
Publication statusPublished - 01-08-1996
Externally publishedYes

Fingerprint

Suid Herpesvirus 1
Suid herpesvirus 1
virion
Virion
glycoproteins
herpes simplex
Simplexvirus
viruses
oligopeptides
mutants
Oligopeptides
Proteins
proteins
virus replication
Open Reading Frames
Sequence Analysis
antiserum
open reading frames
pseudorabies virus glycoproteins
Immune Sera

All Science Journal Classification (ASJC) codes

  • Immunology
  • Virology

Cite this

Dijkstra, Johannes M. ; Visser, Nico ; Mettenleiter, Thomas C. ; Klupp, Barbara G. / Identification and characterization of pseudorabies virus glycoprotein gM as a nonessential virion component. In: Journal of Virology. 1996 ; Vol. 70, No. 8. pp. 5684-5688.
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Identification and characterization of pseudorabies virus glycoprotein gM as a nonessential virion component. / Dijkstra, Johannes M.; Visser, Nico; Mettenleiter, Thomas C.; Klupp, Barbara G.

In: Journal of Virology, Vol. 70, No. 8, 01.08.1996, p. 5684-5688.

Research output: Contribution to journalArticle

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