Abstract
The UL24 gene of herpes simplex virus type 2 (HSV-2) is predicted to encode a 281 amino acid protein with a molecular mass of 30.5 kDa. In this study, the HSV-2 UL24 gene product has been identified by using a rabbit polyclonal antiserum produced against a recombinant protein containing the full-length UL24 gene product of HSV-2 fused to glutathione-S-transferase. The antiserum reacted specifically with a 32 kDa protein in HSV-2 186-infected Vero cells and with 31 and 32 kDa proteins in UL24-expressing Cos-7 cells. Accumulation of UL24 protein to detectable levels required viral DNA synthesis, indicating that the protein was regulated as a late gene. UL24 protein was found to be associated with purified HSV-2 virions and C capsids. Indirect immunofluorescence analysis demonstrated that the UL24-specific fluorescence was detected in perinuclear regions of the cytoplasm and/or in the nucleus as small discrete granules from 9 h post infection (hpi). Furthermore, the UL24 protein expressed singly was detected predominantly in the nucleus and slightly in the cytoplasm at 24 h after transfection, with branch-like cytoplasmic protruding structures. Strong nucleolus staining was visible in partial cells.
| Original language | English |
|---|---|
| Pages (from-to) | 321-327 |
| Number of pages | 7 |
| Journal | Virus Genes |
| Volume | 22 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 09-07-2001 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Genetics
- Virology
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Identification and characterization of the UL24 gene product of herpes simplex virus type 2. / Hong-Yan, Z.; Murata, T.; Goshima, F.; Takakuwa, H.; Koshizuka, T.; Yamauchi, Y.; Nishiyama, Y.
In: Virus Genes, Vol. 22, No. 3, 09.07.2001, p. 321-327.Research output: Contribution to journal › Article
TY - JOUR
T1 - Identification and characterization of the UL24 gene product of herpes simplex virus type 2
AU - Hong-Yan, Z.
AU - Murata, T.
AU - Goshima, F.
AU - Takakuwa, H.
AU - Koshizuka, T.
AU - Yamauchi, Y.
AU - Nishiyama, Y.
PY - 2001/7/9
Y1 - 2001/7/9
N2 - The UL24 gene of herpes simplex virus type 2 (HSV-2) is predicted to encode a 281 amino acid protein with a molecular mass of 30.5 kDa. In this study, the HSV-2 UL24 gene product has been identified by using a rabbit polyclonal antiserum produced against a recombinant protein containing the full-length UL24 gene product of HSV-2 fused to glutathione-S-transferase. The antiserum reacted specifically with a 32 kDa protein in HSV-2 186-infected Vero cells and with 31 and 32 kDa proteins in UL24-expressing Cos-7 cells. Accumulation of UL24 protein to detectable levels required viral DNA synthesis, indicating that the protein was regulated as a late gene. UL24 protein was found to be associated with purified HSV-2 virions and C capsids. Indirect immunofluorescence analysis demonstrated that the UL24-specific fluorescence was detected in perinuclear regions of the cytoplasm and/or in the nucleus as small discrete granules from 9 h post infection (hpi). Furthermore, the UL24 protein expressed singly was detected predominantly in the nucleus and slightly in the cytoplasm at 24 h after transfection, with branch-like cytoplasmic protruding structures. Strong nucleolus staining was visible in partial cells.
AB - The UL24 gene of herpes simplex virus type 2 (HSV-2) is predicted to encode a 281 amino acid protein with a molecular mass of 30.5 kDa. In this study, the HSV-2 UL24 gene product has been identified by using a rabbit polyclonal antiserum produced against a recombinant protein containing the full-length UL24 gene product of HSV-2 fused to glutathione-S-transferase. The antiserum reacted specifically with a 32 kDa protein in HSV-2 186-infected Vero cells and with 31 and 32 kDa proteins in UL24-expressing Cos-7 cells. Accumulation of UL24 protein to detectable levels required viral DNA synthesis, indicating that the protein was regulated as a late gene. UL24 protein was found to be associated with purified HSV-2 virions and C capsids. Indirect immunofluorescence analysis demonstrated that the UL24-specific fluorescence was detected in perinuclear regions of the cytoplasm and/or in the nucleus as small discrete granules from 9 h post infection (hpi). Furthermore, the UL24 protein expressed singly was detected predominantly in the nucleus and slightly in the cytoplasm at 24 h after transfection, with branch-like cytoplasmic protruding structures. Strong nucleolus staining was visible in partial cells.
UR - http://www.scopus.com/inward/record.url?scp=0034950767&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034950767&partnerID=8YFLogxK
U2 - 10.1023/A:1011118424474
DO - 10.1023/A:1011118424474
M3 - Article
C2 - 11450950
AN - SCOPUS:0034950767
VL - 22
SP - 321
EP - 327
JO - Virus Genes
JF - Virus Genes
SN - 0920-8569
IS - 3
ER -