Identification and characterization of the UL24 gene product of herpes simplex virus type 2

Z. Hong-Yan, T. Murata, F. Goshima, H. Takakuwa, T. Koshizuka, Y. Yamauchi, Y. Nishiyama

Research output: Contribution to journalArticle

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Abstract

The UL24 gene of herpes simplex virus type 2 (HSV-2) is predicted to encode a 281 amino acid protein with a molecular mass of 30.5 kDa. In this study, the HSV-2 UL24 gene product has been identified by using a rabbit polyclonal antiserum produced against a recombinant protein containing the full-length UL24 gene product of HSV-2 fused to glutathione-S-transferase. The antiserum reacted specifically with a 32 kDa protein in HSV-2 186-infected Vero cells and with 31 and 32 kDa proteins in UL24-expressing Cos-7 cells. Accumulation of UL24 protein to detectable levels required viral DNA synthesis, indicating that the protein was regulated as a late gene. UL24 protein was found to be associated with purified HSV-2 virions and C capsids. Indirect immunofluorescence analysis demonstrated that the UL24-specific fluorescence was detected in perinuclear regions of the cytoplasm and/or in the nucleus as small discrete granules from 9 h post infection (hpi). Furthermore, the UL24 protein expressed singly was detected predominantly in the nucleus and slightly in the cytoplasm at 24 h after transfection, with branch-like cytoplasmic protruding structures. Strong nucleolus staining was visible in partial cells.

Original languageEnglish
Pages (from-to)321-327
Number of pages7
JournalVirus Genes
Volume22
Issue number3
DOIs
Publication statusPublished - 09-07-2001

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Human Herpesvirus 2
Genes
Proteins
Immune Sera
Cytoplasm
Cytoplasmic Structures
Vero Cells
Capsid
Viral DNA
Indirect Fluorescent Antibody Technique
Glutathione Transferase
Recombinant Proteins
Virion
Transfection
Fluorescence
Staining and Labeling
Rabbits
Amino Acids
Infection

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Virology

Cite this

Hong-Yan, Z., Murata, T., Goshima, F., Takakuwa, H., Koshizuka, T., Yamauchi, Y., & Nishiyama, Y. (2001). Identification and characterization of the UL24 gene product of herpes simplex virus type 2. Virus Genes, 22(3), 321-327. https://doi.org/10.1023/A:1011118424474
Hong-Yan, Z. ; Murata, T. ; Goshima, F. ; Takakuwa, H. ; Koshizuka, T. ; Yamauchi, Y. ; Nishiyama, Y. / Identification and characterization of the UL24 gene product of herpes simplex virus type 2. In: Virus Genes. 2001 ; Vol. 22, No. 3. pp. 321-327.
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Hong-Yan, Z, Murata, T, Goshima, F, Takakuwa, H, Koshizuka, T, Yamauchi, Y & Nishiyama, Y 2001, 'Identification and characterization of the UL24 gene product of herpes simplex virus type 2', Virus Genes, vol. 22, no. 3, pp. 321-327. https://doi.org/10.1023/A:1011118424474

Identification and characterization of the UL24 gene product of herpes simplex virus type 2. / Hong-Yan, Z.; Murata, T.; Goshima, F.; Takakuwa, H.; Koshizuka, T.; Yamauchi, Y.; Nishiyama, Y.

In: Virus Genes, Vol. 22, No. 3, 09.07.2001, p. 321-327.

Research output: Contribution to journalArticle

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