TY - JOUR
T1 - Identification and characterization of the UL24 gene product of herpes simplex virus type 2
AU - Hong-Yan, Z.
AU - Murata, T.
AU - Goshima, F.
AU - Takakuwa, H.
AU - Koshizuka, T.
AU - Yamauchi, Y.
AU - Nishiyama, Y.
N1 - Funding Information:
We thank E. Iwata and T. Tsuruguchi for their technical assistance. This work was supported by a grant from the Japan Sociaty for the Promotion of Science (JSPS-RFTF97L00703) and also by a Grant-in Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.
PY - 2001
Y1 - 2001
N2 - The UL24 gene of herpes simplex virus type 2 (HSV-2) is predicted to encode a 281 amino acid protein with a molecular mass of 30.5 kDa. In this study, the HSV-2 UL24 gene product has been identified by using a rabbit polyclonal antiserum produced against a recombinant protein containing the full-length UL24 gene product of HSV-2 fused to glutathione-S-transferase. The antiserum reacted specifically with a 32 kDa protein in HSV-2 186-infected Vero cells and with 31 and 32 kDa proteins in UL24-expressing Cos-7 cells. Accumulation of UL24 protein to detectable levels required viral DNA synthesis, indicating that the protein was regulated as a late gene. UL24 protein was found to be associated with purified HSV-2 virions and C capsids. Indirect immunofluorescence analysis demonstrated that the UL24-specific fluorescence was detected in perinuclear regions of the cytoplasm and/or in the nucleus as small discrete granules from 9 h post infection (hpi). Furthermore, the UL24 protein expressed singly was detected predominantly in the nucleus and slightly in the cytoplasm at 24 h after transfection, with branch-like cytoplasmic protruding structures. Strong nucleolus staining was visible in partial cells.
AB - The UL24 gene of herpes simplex virus type 2 (HSV-2) is predicted to encode a 281 amino acid protein with a molecular mass of 30.5 kDa. In this study, the HSV-2 UL24 gene product has been identified by using a rabbit polyclonal antiserum produced against a recombinant protein containing the full-length UL24 gene product of HSV-2 fused to glutathione-S-transferase. The antiserum reacted specifically with a 32 kDa protein in HSV-2 186-infected Vero cells and with 31 and 32 kDa proteins in UL24-expressing Cos-7 cells. Accumulation of UL24 protein to detectable levels required viral DNA synthesis, indicating that the protein was regulated as a late gene. UL24 protein was found to be associated with purified HSV-2 virions and C capsids. Indirect immunofluorescence analysis demonstrated that the UL24-specific fluorescence was detected in perinuclear regions of the cytoplasm and/or in the nucleus as small discrete granules from 9 h post infection (hpi). Furthermore, the UL24 protein expressed singly was detected predominantly in the nucleus and slightly in the cytoplasm at 24 h after transfection, with branch-like cytoplasmic protruding structures. Strong nucleolus staining was visible in partial cells.
KW - HSV-2
KW - UL24
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U2 - 10.1023/A:1011118424474
DO - 10.1023/A:1011118424474
M3 - Article
C2 - 11450950
AN - SCOPUS:0034950767
SN - 0920-8569
VL - 22
SP - 321
EP - 327
JO - Virus Genes
JF - Virus Genes
IS - 3
ER -