Abstract
Mannosylation in the endoplasmic reticulum is a key process for synthesizing various glycans. Guanosine diphosphate mannose (GDP-Man) and dolichol phosphate-mannose serve as donor substrates for mannosylation in mammals and are used in N-glycosylation, O-mannosylation, C-mannosylation, and the synthesis of glycosylphosphatidylinositol-anchor (GPI-anchor). Here, we report for the first time that low-abundant uridine diphosphate-mannose (UDP-Man), which can serve as potential donor substrate, exists in mammals. Liquid chromatography-mass spectrometry (LC-MS) analyses showed that mouse brain, especially hypothalamus and neocortex, contains higher concentrations of UDP-Man compared to other organs. In cultured human cell lines, addition of mannose in media increased UDP-Man concentrations in a dose-dependent manner. These findings indicate that in mammals the minor nucleotide sugar UDP-Man regulates glycosylation, especially mannosylation in specific organs or conditions.
| Original language | English |
|---|---|
| Pages (from-to) | 401-407 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 495 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 01-01-2018 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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Identification and characterization of UDP-mannose in human cell lines and mouse organs : Differential distribution across brain regions and organs. / Nakajima, Kazuki; Kizuka, Yasuhiko; Yamaguchi, Yoshiki; Hirabayashi, Yoshio; Takahashi, Kazuo; Yuzawa, Yukio; Taniguchi, Naoyuki.
In: Biochemical and Biophysical Research Communications, Vol. 495, No. 1, 01.01.2018, p. 401-407.Research output: Contribution to journal › Article
TY - JOUR
T1 - Identification and characterization of UDP-mannose in human cell lines and mouse organs
T2 - Differential distribution across brain regions and organs
AU - Nakajima, Kazuki
AU - Kizuka, Yasuhiko
AU - Yamaguchi, Yoshiki
AU - Hirabayashi, Yoshio
AU - Takahashi, Kazuo
AU - Yuzawa, Yukio
AU - Taniguchi, Naoyuki
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Mannosylation in the endoplasmic reticulum is a key process for synthesizing various glycans. Guanosine diphosphate mannose (GDP-Man) and dolichol phosphate-mannose serve as donor substrates for mannosylation in mammals and are used in N-glycosylation, O-mannosylation, C-mannosylation, and the synthesis of glycosylphosphatidylinositol-anchor (GPI-anchor). Here, we report for the first time that low-abundant uridine diphosphate-mannose (UDP-Man), which can serve as potential donor substrate, exists in mammals. Liquid chromatography-mass spectrometry (LC-MS) analyses showed that mouse brain, especially hypothalamus and neocortex, contains higher concentrations of UDP-Man compared to other organs. In cultured human cell lines, addition of mannose in media increased UDP-Man concentrations in a dose-dependent manner. These findings indicate that in mammals the minor nucleotide sugar UDP-Man regulates glycosylation, especially mannosylation in specific organs or conditions.
AB - Mannosylation in the endoplasmic reticulum is a key process for synthesizing various glycans. Guanosine diphosphate mannose (GDP-Man) and dolichol phosphate-mannose serve as donor substrates for mannosylation in mammals and are used in N-glycosylation, O-mannosylation, C-mannosylation, and the synthesis of glycosylphosphatidylinositol-anchor (GPI-anchor). Here, we report for the first time that low-abundant uridine diphosphate-mannose (UDP-Man), which can serve as potential donor substrate, exists in mammals. Liquid chromatography-mass spectrometry (LC-MS) analyses showed that mouse brain, especially hypothalamus and neocortex, contains higher concentrations of UDP-Man compared to other organs. In cultured human cell lines, addition of mannose in media increased UDP-Man concentrations in a dose-dependent manner. These findings indicate that in mammals the minor nucleotide sugar UDP-Man regulates glycosylation, especially mannosylation in specific organs or conditions.
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UR - http://www.scopus.com/inward/citedby.url?scp=85033552961&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2017.10.173
DO - 10.1016/j.bbrc.2017.10.173
M3 - Article
C2 - 29101027
AN - SCOPUS:85033552961
VL - 495
SP - 401
EP - 407
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -