TY - JOUR
T1 - Identification and characterization of UDP-mannose in human cell lines and mouse organs
T2 - Differential distribution across brain regions and organs
AU - Nakajima, Kazuki
AU - Kizuka, Yasuhiko
AU - Yamaguchi, Yoshiki
AU - Hirabayashi, Yoshio
AU - Takahashi, Kazuo
AU - Yuzawa, Yukio
AU - Taniguchi, Naoyuki
N1 - Funding Information:
We thank Dr. Takashi Angata (Academia Sinica, Taiwan) and Dr. Kazuaki Ohtsubo (Kumamoto University) for helpful suggestions on research designs, and Dr. Masanori Kugita (Fujita Health University) and Ms. Mizuki Kurosawa (RIKEN, BSI) for technical support with the animal experiments. This work was supported by Grant-in-Aid for Young Scientists (B), 24790301 (to KN) and Grant-in-Aid for Scientific Research (B), 15H04700 (to NT) from the Ministry of Education, Culture, Sports, Science and Technology of Japan . Appendix A
Publisher Copyright:
© 2017 Elsevier Inc.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Mannosylation in the endoplasmic reticulum is a key process for synthesizing various glycans. Guanosine diphosphate mannose (GDP-Man) and dolichol phosphate-mannose serve as donor substrates for mannosylation in mammals and are used in N-glycosylation, O-mannosylation, C-mannosylation, and the synthesis of glycosylphosphatidylinositol-anchor (GPI-anchor). Here, we report for the first time that low-abundant uridine diphosphate-mannose (UDP-Man), which can serve as potential donor substrate, exists in mammals. Liquid chromatography-mass spectrometry (LC-MS) analyses showed that mouse brain, especially hypothalamus and neocortex, contains higher concentrations of UDP-Man compared to other organs. In cultured human cell lines, addition of mannose in media increased UDP-Man concentrations in a dose-dependent manner. These findings indicate that in mammals the minor nucleotide sugar UDP-Man regulates glycosylation, especially mannosylation in specific organs or conditions.
AB - Mannosylation in the endoplasmic reticulum is a key process for synthesizing various glycans. Guanosine diphosphate mannose (GDP-Man) and dolichol phosphate-mannose serve as donor substrates for mannosylation in mammals and are used in N-glycosylation, O-mannosylation, C-mannosylation, and the synthesis of glycosylphosphatidylinositol-anchor (GPI-anchor). Here, we report for the first time that low-abundant uridine diphosphate-mannose (UDP-Man), which can serve as potential donor substrate, exists in mammals. Liquid chromatography-mass spectrometry (LC-MS) analyses showed that mouse brain, especially hypothalamus and neocortex, contains higher concentrations of UDP-Man compared to other organs. In cultured human cell lines, addition of mannose in media increased UDP-Man concentrations in a dose-dependent manner. These findings indicate that in mammals the minor nucleotide sugar UDP-Man regulates glycosylation, especially mannosylation in specific organs or conditions.
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U2 - 10.1016/j.bbrc.2017.10.173
DO - 10.1016/j.bbrc.2017.10.173
M3 - Article
C2 - 29101027
AN - SCOPUS:85033552961
VL - 495
SP - 401
EP - 407
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -