Identification and characterization of UDP-mannose in human cell lines and mouse organs: Differential distribution across brain regions and organs

Kazuki Nakajima, Yasuhiko Kizuka, Yoshiki Yamaguchi, Yoshio Hirabayashi, Kazuo Takahashi, Yukio Yuzawa, Naoyuki Taniguchi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Mannosylation in the endoplasmic reticulum is a key process for synthesizing various glycans. Guanosine diphosphate mannose (GDP-Man) and dolichol phosphate-mannose serve as donor substrates for mannosylation in mammals and are used in N-glycosylation, O-mannosylation, C-mannosylation, and the synthesis of glycosylphosphatidylinositol-anchor (GPI-anchor). Here, we report for the first time that low-abundant uridine diphosphate-mannose (UDP-Man), which can serve as potential donor substrate, exists in mammals. Liquid chromatography-mass spectrometry (LC-MS) analyses showed that mouse brain, especially hypothalamus and neocortex, contains higher concentrations of UDP-Man compared to other organs. In cultured human cell lines, addition of mannose in media increased UDP-Man concentrations in a dose-dependent manner. These findings indicate that in mammals the minor nucleotide sugar UDP-Man regulates glycosylation, especially mannosylation in specific organs or conditions.

Original languageEnglish
Pages (from-to)401-407
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume495
Issue number1
DOIs
Publication statusPublished - 01-01-2018

Fingerprint

Mammals
Brain
Cells
Glycosylation
Cell Line
Mannose
Dolichol Phosphates
Guanosine Diphosphate Mannose
Glycosylphosphatidylinositols
Neocortex
Liquid chromatography
Substrates
Sugars
Liquid Chromatography
Endoplasmic Reticulum
Hypothalamus
Mass spectrometry
Polysaccharides
Cultured Cells
Mass Spectrometry

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

@article{f5a61ba5aef94a358073c48be491bce6,
title = "Identification and characterization of UDP-mannose in human cell lines and mouse organs: Differential distribution across brain regions and organs",
abstract = "Mannosylation in the endoplasmic reticulum is a key process for synthesizing various glycans. Guanosine diphosphate mannose (GDP-Man) and dolichol phosphate-mannose serve as donor substrates for mannosylation in mammals and are used in N-glycosylation, O-mannosylation, C-mannosylation, and the synthesis of glycosylphosphatidylinositol-anchor (GPI-anchor). Here, we report for the first time that low-abundant uridine diphosphate-mannose (UDP-Man), which can serve as potential donor substrate, exists in mammals. Liquid chromatography-mass spectrometry (LC-MS) analyses showed that mouse brain, especially hypothalamus and neocortex, contains higher concentrations of UDP-Man compared to other organs. In cultured human cell lines, addition of mannose in media increased UDP-Man concentrations in a dose-dependent manner. These findings indicate that in mammals the minor nucleotide sugar UDP-Man regulates glycosylation, especially mannosylation in specific organs or conditions.",
author = "Kazuki Nakajima and Yasuhiko Kizuka and Yoshiki Yamaguchi and Yoshio Hirabayashi and Kazuo Takahashi and Yukio Yuzawa and Naoyuki Taniguchi",
year = "2018",
month = "1",
day = "1",
doi = "10.1016/j.bbrc.2017.10.173",
language = "English",
volume = "495",
pages = "401--407",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

Identification and characterization of UDP-mannose in human cell lines and mouse organs : Differential distribution across brain regions and organs. / Nakajima, Kazuki; Kizuka, Yasuhiko; Yamaguchi, Yoshiki; Hirabayashi, Yoshio; Takahashi, Kazuo; Yuzawa, Yukio; Taniguchi, Naoyuki.

In: Biochemical and Biophysical Research Communications, Vol. 495, No. 1, 01.01.2018, p. 401-407.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Identification and characterization of UDP-mannose in human cell lines and mouse organs

T2 - Differential distribution across brain regions and organs

AU - Nakajima, Kazuki

AU - Kizuka, Yasuhiko

AU - Yamaguchi, Yoshiki

AU - Hirabayashi, Yoshio

AU - Takahashi, Kazuo

AU - Yuzawa, Yukio

AU - Taniguchi, Naoyuki

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Mannosylation in the endoplasmic reticulum is a key process for synthesizing various glycans. Guanosine diphosphate mannose (GDP-Man) and dolichol phosphate-mannose serve as donor substrates for mannosylation in mammals and are used in N-glycosylation, O-mannosylation, C-mannosylation, and the synthesis of glycosylphosphatidylinositol-anchor (GPI-anchor). Here, we report for the first time that low-abundant uridine diphosphate-mannose (UDP-Man), which can serve as potential donor substrate, exists in mammals. Liquid chromatography-mass spectrometry (LC-MS) analyses showed that mouse brain, especially hypothalamus and neocortex, contains higher concentrations of UDP-Man compared to other organs. In cultured human cell lines, addition of mannose in media increased UDP-Man concentrations in a dose-dependent manner. These findings indicate that in mammals the minor nucleotide sugar UDP-Man regulates glycosylation, especially mannosylation in specific organs or conditions.

AB - Mannosylation in the endoplasmic reticulum is a key process for synthesizing various glycans. Guanosine diphosphate mannose (GDP-Man) and dolichol phosphate-mannose serve as donor substrates for mannosylation in mammals and are used in N-glycosylation, O-mannosylation, C-mannosylation, and the synthesis of glycosylphosphatidylinositol-anchor (GPI-anchor). Here, we report for the first time that low-abundant uridine diphosphate-mannose (UDP-Man), which can serve as potential donor substrate, exists in mammals. Liquid chromatography-mass spectrometry (LC-MS) analyses showed that mouse brain, especially hypothalamus and neocortex, contains higher concentrations of UDP-Man compared to other organs. In cultured human cell lines, addition of mannose in media increased UDP-Man concentrations in a dose-dependent manner. These findings indicate that in mammals the minor nucleotide sugar UDP-Man regulates glycosylation, especially mannosylation in specific organs or conditions.

UR - http://www.scopus.com/inward/record.url?scp=85033552961&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85033552961&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2017.10.173

DO - 10.1016/j.bbrc.2017.10.173

M3 - Article

C2 - 29101027

AN - SCOPUS:85033552961

VL - 495

SP - 401

EP - 407

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -