Identification, isolation, and characterization of mesenchymal progenitors in mouse and human skeletal muscle

Akiyoshi Uezumi, Takehiro Kasai, Kunihiro Tsuchida

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

Mesenchymal progenitors residing in the muscle interstitial space contribute to pathogeneses such as fat infiltration and fibrosis. Because fat infiltration and fibrosis are hallmarks of diseased muscle, it is important to establish an accurate and reproducible method for isolating mesenchymal progenitors for research on muscle diseases. In this chapter, we describe methods based on fluorescence-activated cell sorting (FACS) to purify mesenchymal progenitors from mouse and human skeletal muscle using the most reliable marker for mesenchymal progenitors, PDGFRα. These methods allow concurrent isolation of the muscle stem cells called satellite cells. The quality of isolated mesenchymal progenitors is confirmed by their remarkable adipogenic potential without myogenic capacity, while purified satellite cells possess robust myogenic activity with no adipogenic potential. Simultaneous isolation of both mesenchymal progenitors and satellite cells from mouse and human tissues provides a powerful platform for studying skeletal muscle regeneration and diseases.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages241-253
Number of pages13
DOIs
Publication statusPublished - 01-01-2016

Publication series

NameMethods in Molecular Biology
Volume1460
ISSN (Print)1064-3745

Fingerprint

Skeletal Muscle
Muscles
Fibrosis
Fats
Mesenchymal Stromal Cells
Muscle Cells
Regeneration
Flow Cytometry
Stem Cells
Research

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Uezumi, A., Kasai, T., & Tsuchida, K. (2016). Identification, isolation, and characterization of mesenchymal progenitors in mouse and human skeletal muscle. In Methods in Molecular Biology (pp. 241-253). (Methods in Molecular Biology; Vol. 1460). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3810-0_17
Uezumi, Akiyoshi ; Kasai, Takehiro ; Tsuchida, Kunihiro. / Identification, isolation, and characterization of mesenchymal progenitors in mouse and human skeletal muscle. Methods in Molecular Biology. Humana Press Inc., 2016. pp. 241-253 (Methods in Molecular Biology).
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Uezumi, A, Kasai, T & Tsuchida, K 2016, Identification, isolation, and characterization of mesenchymal progenitors in mouse and human skeletal muscle. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1460, Humana Press Inc., pp. 241-253. https://doi.org/10.1007/978-1-4939-3810-0_17

Identification, isolation, and characterization of mesenchymal progenitors in mouse and human skeletal muscle. / Uezumi, Akiyoshi; Kasai, Takehiro; Tsuchida, Kunihiro.

Methods in Molecular Biology. Humana Press Inc., 2016. p. 241-253 (Methods in Molecular Biology; Vol. 1460).

Research output: Chapter in Book/Report/Conference proceedingChapter

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Uezumi A, Kasai T, Tsuchida K. Identification, isolation, and characterization of mesenchymal progenitors in mouse and human skeletal muscle. In Methods in Molecular Biology. Humana Press Inc. 2016. p. 241-253. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3810-0_17