Identification of a GPI-anchored type HDL-binding protein on human macrophages

Akifumi Matsuyama, Shizuya Yamashita, Naohiko Sakai, Takao Maruyama, Eiko Okuda, Ken Ichi Hirano, Shinji Kihara, Hisatoyo Hiraoka, Yuji Matsuzawa

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Abstract

To identify the HDL3-binding proteins on human macrophages, we examined the involvement of GPI-anchored protein in the binding of HDL3, and tried to purify HDL3-binding protein. From membrane fractions of macrophages, we obtained 80- and 130-kDa HDL3-binding proteins by ligand blotting. Treatment of macrophages with phosphatidylinositol-specific phospholipase C (PI-PLC) significantly decreased the specific HDL3-binding in a dose-dependent manner. Furthermore, treatment with mannosamine, which blocks GPI-anchor formation, decreased specific HDL3-binding in a dose-dependent manner. PI-PLC treatment released from the cells the proteins with an M(r) of 80 kDa, which could also bind HDL3. PI-PLC as well as mannosamine treatment markedly reduced cholesterol efflux from macrophages in association with the decreased HDL-binding. Using HDL3-affinity chromatography, we purified 80-kDa GPI-anchored type HDL3-binding protein. In summary, we demonstrate the implication of 80-kDa GPI-anchored protein in the binding of HDL3 to human macrophages, which might have some role in reverse cholesterol transport. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)864-871
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume272
Issue number3
DOIs
Publication statusPublished - 16-06-2000

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Matsuyama, A., Yamashita, S., Sakai, N., Maruyama, T., Okuda, E., Hirano, K. I., Kihara, S., Hiraoka, H., & Matsuzawa, Y. (2000). Identification of a GPI-anchored type HDL-binding protein on human macrophages. Biochemical and Biophysical Research Communications, 272(3), 864-871. https://doi.org/10.1006/bbrc.2000.2855