Identification of a GPI-anchored type HDL-binding protein on human macrophages

Akifumi Matsuyama, Shizuya Yamashita, Naohiko Sakai, Takao Maruyama, Eiko Okuda, Ken Ichi Hirano, Shinji Kihara, Hisatoyo Hiraoka, Yuji Matsuzawa

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To identify the HDL3-binding proteins on human macrophages, we examined the involvement of GPI-anchored protein in the binding of HDL3, and tried to purify HDL3-binding protein. From membrane fractions of macrophages, we obtained 80- and 130-kDa HDL3-binding proteins by ligand blotting. Treatment of macrophages with phosphatidylinositol-specific phospholipase C (PI-PLC) significantly decreased the specific HDL3-binding in a dose-dependent manner. Furthermore, treatment with mannosamine, which blocks GPI-anchor formation, decreased specific HDL3-binding in a dose-dependent manner. PI-PLC treatment released from the cells the proteins with an M(r) of 80 kDa, which could also bind HDL3. PI-PLC as well as mannosamine treatment markedly reduced cholesterol efflux from macrophages in association with the decreased HDL-binding. Using HDL3-affinity chromatography, we purified 80-kDa GPI-anchored type HDL3-binding protein. In summary, we demonstrate the implication of 80-kDa GPI-anchored protein in the binding of HDL3 to human macrophages, which might have some role in reverse cholesterol transport. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)864-871
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume272
Issue number3
DOIs
Publication statusPublished - 16-06-2000

Fingerprint

Macrophages
Phosphoinositide Phospholipase C
Carrier Proteins
Protein Binding
Cholesterol
Affinity chromatography
Proteins
Anchors
Affinity Chromatography
high density lipoprotein binding protein
Association reactions
Ligands
Membranes
mannosamine

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Matsuyama, Akifumi ; Yamashita, Shizuya ; Sakai, Naohiko ; Maruyama, Takao ; Okuda, Eiko ; Hirano, Ken Ichi ; Kihara, Shinji ; Hiraoka, Hisatoyo ; Matsuzawa, Yuji. / Identification of a GPI-anchored type HDL-binding protein on human macrophages. In: Biochemical and Biophysical Research Communications. 2000 ; Vol. 272, No. 3. pp. 864-871.
@article{cf2b51bce9614c35b891a4df31c047ce,
title = "Identification of a GPI-anchored type HDL-binding protein on human macrophages",
abstract = "To identify the HDL3-binding proteins on human macrophages, we examined the involvement of GPI-anchored protein in the binding of HDL3, and tried to purify HDL3-binding protein. From membrane fractions of macrophages, we obtained 80- and 130-kDa HDL3-binding proteins by ligand blotting. Treatment of macrophages with phosphatidylinositol-specific phospholipase C (PI-PLC) significantly decreased the specific HDL3-binding in a dose-dependent manner. Furthermore, treatment with mannosamine, which blocks GPI-anchor formation, decreased specific HDL3-binding in a dose-dependent manner. PI-PLC treatment released from the cells the proteins with an M(r) of 80 kDa, which could also bind HDL3. PI-PLC as well as mannosamine treatment markedly reduced cholesterol efflux from macrophages in association with the decreased HDL-binding. Using HDL3-affinity chromatography, we purified 80-kDa GPI-anchored type HDL3-binding protein. In summary, we demonstrate the implication of 80-kDa GPI-anchored protein in the binding of HDL3 to human macrophages, which might have some role in reverse cholesterol transport. (C) 2000 Academic Press.",
author = "Akifumi Matsuyama and Shizuya Yamashita and Naohiko Sakai and Takao Maruyama and Eiko Okuda and Hirano, {Ken Ichi} and Shinji Kihara and Hisatoyo Hiraoka and Yuji Matsuzawa",
year = "2000",
month = "6",
day = "16",
doi = "10.1006/bbrc.2000.2855",
language = "English",
volume = "272",
pages = "864--871",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

Matsuyama, A, Yamashita, S, Sakai, N, Maruyama, T, Okuda, E, Hirano, KI, Kihara, S, Hiraoka, H & Matsuzawa, Y 2000, 'Identification of a GPI-anchored type HDL-binding protein on human macrophages', Biochemical and Biophysical Research Communications, vol. 272, no. 3, pp. 864-871. https://doi.org/10.1006/bbrc.2000.2855

Identification of a GPI-anchored type HDL-binding protein on human macrophages. / Matsuyama, Akifumi; Yamashita, Shizuya; Sakai, Naohiko; Maruyama, Takao; Okuda, Eiko; Hirano, Ken Ichi; Kihara, Shinji; Hiraoka, Hisatoyo; Matsuzawa, Yuji.

In: Biochemical and Biophysical Research Communications, Vol. 272, No. 3, 16.06.2000, p. 864-871.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Identification of a GPI-anchored type HDL-binding protein on human macrophages

AU - Matsuyama, Akifumi

AU - Yamashita, Shizuya

AU - Sakai, Naohiko

AU - Maruyama, Takao

AU - Okuda, Eiko

AU - Hirano, Ken Ichi

AU - Kihara, Shinji

AU - Hiraoka, Hisatoyo

AU - Matsuzawa, Yuji

PY - 2000/6/16

Y1 - 2000/6/16

N2 - To identify the HDL3-binding proteins on human macrophages, we examined the involvement of GPI-anchored protein in the binding of HDL3, and tried to purify HDL3-binding protein. From membrane fractions of macrophages, we obtained 80- and 130-kDa HDL3-binding proteins by ligand blotting. Treatment of macrophages with phosphatidylinositol-specific phospholipase C (PI-PLC) significantly decreased the specific HDL3-binding in a dose-dependent manner. Furthermore, treatment with mannosamine, which blocks GPI-anchor formation, decreased specific HDL3-binding in a dose-dependent manner. PI-PLC treatment released from the cells the proteins with an M(r) of 80 kDa, which could also bind HDL3. PI-PLC as well as mannosamine treatment markedly reduced cholesterol efflux from macrophages in association with the decreased HDL-binding. Using HDL3-affinity chromatography, we purified 80-kDa GPI-anchored type HDL3-binding protein. In summary, we demonstrate the implication of 80-kDa GPI-anchored protein in the binding of HDL3 to human macrophages, which might have some role in reverse cholesterol transport. (C) 2000 Academic Press.

AB - To identify the HDL3-binding proteins on human macrophages, we examined the involvement of GPI-anchored protein in the binding of HDL3, and tried to purify HDL3-binding protein. From membrane fractions of macrophages, we obtained 80- and 130-kDa HDL3-binding proteins by ligand blotting. Treatment of macrophages with phosphatidylinositol-specific phospholipase C (PI-PLC) significantly decreased the specific HDL3-binding in a dose-dependent manner. Furthermore, treatment with mannosamine, which blocks GPI-anchor formation, decreased specific HDL3-binding in a dose-dependent manner. PI-PLC treatment released from the cells the proteins with an M(r) of 80 kDa, which could also bind HDL3. PI-PLC as well as mannosamine treatment markedly reduced cholesterol efflux from macrophages in association with the decreased HDL-binding. Using HDL3-affinity chromatography, we purified 80-kDa GPI-anchored type HDL3-binding protein. In summary, we demonstrate the implication of 80-kDa GPI-anchored protein in the binding of HDL3 to human macrophages, which might have some role in reverse cholesterol transport. (C) 2000 Academic Press.

UR - http://www.scopus.com/inward/record.url?scp=0034674315&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034674315&partnerID=8YFLogxK

U2 - 10.1006/bbrc.2000.2855

DO - 10.1006/bbrc.2000.2855

M3 - Article

C2 - 10860843

AN - SCOPUS:0034674315

VL - 272

SP - 864

EP - 871

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -