Identification of a novel Cdc42 GEF that is localized to the PAT-3-mediated adhesive structure

Takao Hikita, Hiroshi Qadota, Daisuke Tsuboi, Shinichiro Taya, Donald G. Moerman, Kozo Kaibuchi

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

In the model organism Caenorhabditis elegans, UNC-112 is colocalized with PAT-3/β-integrin and is a critical protein in the formation of PAT-3-mediated adhesive structure in body-wall muscle cells. However, the signaling pathway downstream of PAT-3/UNC-112 is largely unknown. To clarify the signaling pathway from PAT-3/UNC-112 to the actin cytoskeleton, we searched for and identified a novel Dbl homology/pleckstrin homology (DH/PH) domain containing protein, UIG-1 (UNC-112-interacting guanine nucleotide exchange factor-1). UIG-1 was colocalized with UNC-112 at dense bodies in body-wall muscle cells. UIG-1 showed CDC-42-specific GEF activity in vitro and induced filopodia formation in NIH 3T3 cells. Depletion of CDC-42 or PAT-3 in the developmental stage, by RNAi, prevented the formation of continuous actin filament in body-wall muscle cells. Taken together, these results suggest that UIG-1 links a PAT-3/UNC-112 complex to the CDC-42 signaling pathway during muscle formation.

Original languageEnglish
Pages (from-to)139-145
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume335
Issue number1
DOIs
Publication statusPublished - 16-09-2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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