TY - JOUR
T1 - Identification of a novel Cdc42 GEF that is localized to the PAT-3-mediated adhesive structure
AU - Hikita, Takao
AU - Qadota, Hiroshi
AU - Tsuboi, Daisuke
AU - Taya, Shinichiro
AU - Moerman, Donald G.
AU - Kaibuchi, Kozo
N1 - Funding Information:
We thank Dr. Robert Barstead (Oklahoma Medical Research Foundation, Evanston, IL) for the cDNA library; Dr. A. Fire for providing GEP plasmids; Caenorhabditis Genetic Center (University of Minnesota, MN, USA) for C.elegans strains, and Ms T. Ishii for secretarial assistance. This work was supported by Grants-in-Aid for scientific research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) and The 21st Century Centre of Excellence (COE) Program from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT).
PY - 2005/9/16
Y1 - 2005/9/16
N2 - In the model organism Caenorhabditis elegans, UNC-112 is colocalized with PAT-3/β-integrin and is a critical protein in the formation of PAT-3-mediated adhesive structure in body-wall muscle cells. However, the signaling pathway downstream of PAT-3/UNC-112 is largely unknown. To clarify the signaling pathway from PAT-3/UNC-112 to the actin cytoskeleton, we searched for and identified a novel Dbl homology/pleckstrin homology (DH/PH) domain containing protein, UIG-1 (UNC-112-interacting guanine nucleotide exchange factor-1). UIG-1 was colocalized with UNC-112 at dense bodies in body-wall muscle cells. UIG-1 showed CDC-42-specific GEF activity in vitro and induced filopodia formation in NIH 3T3 cells. Depletion of CDC-42 or PAT-3 in the developmental stage, by RNAi, prevented the formation of continuous actin filament in body-wall muscle cells. Taken together, these results suggest that UIG-1 links a PAT-3/UNC-112 complex to the CDC-42 signaling pathway during muscle formation.
AB - In the model organism Caenorhabditis elegans, UNC-112 is colocalized with PAT-3/β-integrin and is a critical protein in the formation of PAT-3-mediated adhesive structure in body-wall muscle cells. However, the signaling pathway downstream of PAT-3/UNC-112 is largely unknown. To clarify the signaling pathway from PAT-3/UNC-112 to the actin cytoskeleton, we searched for and identified a novel Dbl homology/pleckstrin homology (DH/PH) domain containing protein, UIG-1 (UNC-112-interacting guanine nucleotide exchange factor-1). UIG-1 was colocalized with UNC-112 at dense bodies in body-wall muscle cells. UIG-1 showed CDC-42-specific GEF activity in vitro and induced filopodia formation in NIH 3T3 cells. Depletion of CDC-42 or PAT-3 in the developmental stage, by RNAi, prevented the formation of continuous actin filament in body-wall muscle cells. Taken together, these results suggest that UIG-1 links a PAT-3/UNC-112 complex to the CDC-42 signaling pathway during muscle formation.
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U2 - 10.1016/j.bbrc.2005.07.068
DO - 10.1016/j.bbrc.2005.07.068
M3 - Article
C2 - 16055082
AN - SCOPUS:25144506641
VL - 335
SP - 139
EP - 145
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -