Identification of a novel component C2ORF3 in the lariat-intron complex: Lack of C2ORF3 interferes with pre-mRNA splicing via intron turnover pathway

Rei Yoshimoto, Katsuya Okawa, Minoru Yoshida, Mutsuhito Ohno, Naoyuki Kataoka

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

To identify the novel factors involved in the postsplicing intron turnover pathway, we carried out immunoprecipitation with known postsplicing factors, hPrp43 and TFIP11. As an interacting factor, we identified C2ORF3 protein by mass spectrometry. We found that C2ORF3 protein is present in the previously characterized Intron Large (IL) complex with an excised lariat intron. In vitro splicing using C2ORF3-depleted nuclear extracts showed significant repression of splicing, suggesting that C2ORF3 protein is required for pre-mRNA splicing through its presumable role in efficient intron turnover. Interestingly, C2ORF3 protein is localized in both the nucleoplasm and nucleoli, which suggests a potential function in rRNA processing.

Original languageEnglish
Pages (from-to)78-87
Number of pages10
JournalGenes to Cells
Volume19
Issue number1
DOIs
Publication statusPublished - 01-2014

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

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