TY - JOUR
T1 - Identification of a novel, tissue-specific calpain htra-3; a human homologue of the Caenorhabditis elegans sex determination gene
AU - Mugita, Norifumi
AU - Kimura, Yoriyoshi
AU - Ogawa, Michio
AU - Saya, Hideyuki
AU - Nakao, Mitsuyoshi
N1 - Funding Information:
We thank Dr. Q. Hu for providing the pCGN expression vector, Dr. J. Moon for checking the manuscript, and K. Uriuda for secretarial assistance. H.S. and M.N. were supported by a grant for basic research from the Ministry of Education, Science and Culture of Japan.
PY - 1997/10/29
Y1 - 1997/10/29
N2 - We cloned a novel human gene encoding a tissue-specific calpain, termed htra-3, which is highly homologous to the tra-3 sex determination gene of Caenorhabditis elegans. The predicted htra-3 polypeptide had similarity to the calpain large subunits in domain organization throughout domains I to III, but the sequences of domain IV lacked calcium-binding motifs. Northern blot analysis revealed high expression in the colon, small intestine and testis. Radiation hybrid mapping localized the htra-3 gene to chromosome 11q14 (2.53cR apart from WI-3895). Western blot analysis demonstrated that the approximately 73-kDa htra-3 protein was transiently expressed in COS-7 cells. These observations, together with the genetic information in C. elegans, suggest a unique function for htra-3 protein.
AB - We cloned a novel human gene encoding a tissue-specific calpain, termed htra-3, which is highly homologous to the tra-3 sex determination gene of Caenorhabditis elegans. The predicted htra-3 polypeptide had similarity to the calpain large subunits in domain organization throughout domains I to III, but the sequences of domain IV lacked calcium-binding motifs. Northern blot analysis revealed high expression in the colon, small intestine and testis. Radiation hybrid mapping localized the htra-3 gene to chromosome 11q14 (2.53cR apart from WI-3895). Western blot analysis demonstrated that the approximately 73-kDa htra-3 protein was transiently expressed in COS-7 cells. These observations, together with the genetic information in C. elegans, suggest a unique function for htra-3 protein.
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U2 - 10.1006/bbrc.1997.7571
DO - 10.1006/bbrc.1997.7571
M3 - Article
C2 - 9367857
AN - SCOPUS:0031590410
SN - 0006-291X
VL - 239
SP - 845
EP - 850
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -