Identification of a Shiga-toxin type I variant containing an IS1203-like element, from Shiga-toxin producing Escherichia coli O157:H7

Masahiro Suzuki, Fumio Kondo, Yuko Ito, Masakado Matsumoto, Mami Hata, Hisao Oka, Masao Takahashi, Kenji Sakae

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We found two Shiga toxin producing Escherichia coli O157:H7 strains isolated from humans carrying the stx1 gene with an IS1203-like element (designated as IS1203v1). The IS1203v1 was inserted into the coding region of the A subunit 7 bp upstream from the TGA termination codon, resulting in a loss of two amino acid residues (Ser-Ser) from its C terminus. Toxicity of the Stx1 was confirmed by Vero cell assay. IS1203v1 hardly affected the stx1 gene in either its expression or the toxicity of its product.

Original languageEnglish
Pages (from-to)63-67
Number of pages5
JournalFEMS Microbiology Letters
Volume234
Issue number1
DOIs
Publication statusPublished - 01-05-2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

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