Identification of a Shiga-toxin type I variant containing an IS1203-like element, from Shiga-toxin producing Escherichia coli O157:H7

Masahiro Suzuki; Fumio Kondo; Yuko Ito; Masakado Matsumoto; Mami Hata; Hisao Oka; Masao Takahashi; Kenji Sakae

doi:10.1016/j.femsle.2004.03.008

Identification of a Shiga-toxin type I variant containing an IS1203-like element, from Shiga-toxin producing Escherichia coli O157:H7

Masahiro Suzuki
, Fumio Kondo
, Yuko Ito
, Masakado Matsumoto
, Mami Hata
, Hisao Oka
, Masao Takahashi
, Kenji Sakae

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

We found two Shiga toxin producing Escherichia coli O157:H7 strains isolated from humans carrying the stx₁ gene with an IS1203-like element (designated as IS1203v₁). The IS1203v₁ was inserted into the coding region of the A subunit 7 bp upstream from the TGA termination codon, resulting in a loss of two amino acid residues (Ser-Ser) from its C terminus. Toxicity of the Stx1 was confirmed by Vero cell assay. IS1203v₁ hardly affected the stx₁ gene in either its expression or the toxicity of its product.

Original language	English
Pages (from-to)	63-67
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	234
Issue number	1
DOIs	https://doi.org/10.1016/j.femsle.2004.03.008
Publication status	Published - 01-05-2004
Externally published	Yes

All Science Journal Classification (ASJC) codes

Microbiology
Molecular Biology
Genetics

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10.1016/j.femsle.2004.03.008

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title = "Identification of a Shiga-toxin type I variant containing an IS1203-like element, from Shiga-toxin producing Escherichia coli O157:H7",

abstract = "We found two Shiga toxin producing Escherichia coli O157:H7 strains isolated from humans carrying the stx1 gene with an IS1203-like element (designated as IS1203v1). The IS1203v1 was inserted into the coding region of the A subunit 7 bp upstream from the TGA termination codon, resulting in a loss of two amino acid residues (Ser-Ser) from its C terminus. Toxicity of the Stx1 was confirmed by Vero cell assay. IS1203v1 hardly affected the stx1 gene in either its expression or the toxicity of its product.",

author = "Masahiro Suzuki and Fumio Kondo and Yuko Ito and Masakado Matsumoto and Mami Hata and Hisao Oka and Masao Takahashi and Kenji Sakae",

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doi = "10.1016/j.femsle.2004.03.008",

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T1 - Identification of a Shiga-toxin type I variant containing an IS1203-like element, from Shiga-toxin producing Escherichia coli O157:H7

AU - Suzuki, Masahiro

AU - Kondo, Fumio

AU - Ito, Yuko

AU - Matsumoto, Masakado

AU - Hata, Mami

AU - Oka, Hisao

AU - Takahashi, Masao

AU - Sakae, Kenji

PY - 2004/5/1

Y1 - 2004/5/1

N2 - We found two Shiga toxin producing Escherichia coli O157:H7 strains isolated from humans carrying the stx1 gene with an IS1203-like element (designated as IS1203v1). The IS1203v1 was inserted into the coding region of the A subunit 7 bp upstream from the TGA termination codon, resulting in a loss of two amino acid residues (Ser-Ser) from its C terminus. Toxicity of the Stx1 was confirmed by Vero cell assay. IS1203v1 hardly affected the stx1 gene in either its expression or the toxicity of its product.

AB - We found two Shiga toxin producing Escherichia coli O157:H7 strains isolated from humans carrying the stx1 gene with an IS1203-like element (designated as IS1203v1). The IS1203v1 was inserted into the coding region of the A subunit 7 bp upstream from the TGA termination codon, resulting in a loss of two amino acid residues (Ser-Ser) from its C terminus. Toxicity of the Stx1 was confirmed by Vero cell assay. IS1203v1 hardly affected the stx1 gene in either its expression or the toxicity of its product.

UR - https://www.scopus.com/pages/publications/1942438121

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DO - 10.1016/j.femsle.2004.03.008

M3 - Article

C2 - 15109720

AN - SCOPUS:1942438121

SN - 0378-1097

VL - 234

SP - 63

EP - 67

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1

ER -

Identification of a Shiga-toxin type I variant containing an IS1203-like element, from Shiga-toxin producing Escherichia coli O157:H7

Abstract

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