Identification of additional quantitative trait loci (QTL) responsible for susceptibility to infectious pancreatic necrosis virus in rainbow trout

Akiyuki Ozaki, Sok Kean Khoo, Yasutoshi Yoshiura, Mitsuru Ototake, Takashi Sakamoto, J・m Dijkstra, Nobuaki Okamoto

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Previous typing of microsatellite markers in a BC1F1 backcross rainbow trout Oncorhynchus mykiss revealed two quantitative trait loci (QTL) with a significant impact on susceptibility to infectious pancreatic necrosis. In the present study, additional genetic markers were mapped using the same strain in order to increase QTL resolution. A total of 199 individuals were screened at 226 marker loci (106 microsatellites, 116 amplified fragment length polymorphism markers, classical major histocompatibility complex molecules [MHC class I, nonclassical MHC class I, MHC class II] and tyrosinase) and the QTL were determined by single-point and interval mappings. Seven QTL were distinguished in the linkage groups RT-11, 12, 17, 23, 26, 29 and 31, in addition to the two already known in RT-3 and 22. The major QTL were those in RT-3, 12 and 22; whereas the classical MHC class I locus Onmy-UBA and the MHC class II locus Onmy-DAB were not associated with any of the QTL, a nonclassical MHC class I region with Onmy-UCA, Onmy-UDA, and Onmy-UEA mapped to the major QTL on RT-3.

Original languageEnglish
Pages (from-to)131-140
Number of pages10
JournalFish Pathology
Volume42
Issue number3
DOIs
Publication statusPublished - 01-09-2007

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Infectious pancreatic necrosis virus
rainbow
Oncorhynchus mykiss
quantitative trait loci
virus
major histocompatibility complex
genetic marker
polymorphism
loci
microsatellite repeats
necrosis
marker
linkage groups
amplified fragment length polymorphism
genetic markers

All Science Journal Classification (ASJC) codes

  • Aquatic Science
  • Animal Science and Zoology

Cite this

Ozaki, Akiyuki ; Khoo, Sok Kean ; Yoshiura, Yasutoshi ; Ototake, Mitsuru ; Sakamoto, Takashi ; Dijkstra, J・m ; Okamoto, Nobuaki. / Identification of additional quantitative trait loci (QTL) responsible for susceptibility to infectious pancreatic necrosis virus in rainbow trout. In: Fish Pathology. 2007 ; Vol. 42, No. 3. pp. 131-140.
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Identification of additional quantitative trait loci (QTL) responsible for susceptibility to infectious pancreatic necrosis virus in rainbow trout. / Ozaki, Akiyuki; Khoo, Sok Kean; Yoshiura, Yasutoshi; Ototake, Mitsuru; Sakamoto, Takashi; Dijkstra, J・m; Okamoto, Nobuaki.

In: Fish Pathology, Vol. 42, No. 3, 01.09.2007, p. 131-140.

Research output: Contribution to journalArticle

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