TY - JOUR
T1 - Identification of additional quantitative trait loci (QTL) responsible for susceptibility to infectious pancreatic necrosis virus in rainbow trout
AU - Ozaki, Akiyuki
AU - Khoo, Sok Kean
AU - Yoshiura, Yasutoshi
AU - Ototake, Mitsuru
AU - Sakamoto, Takashi
AU - Dijkstra, Johannes Martinus
AU - Okamoto, Nobuaki
PY - 2007/9
Y1 - 2007/9
N2 - Previous typing of microsatellite markers in a BC1F1 backcross rainbow trout Oncorhynchus mykiss revealed two quantitative trait loci (QTL) with a significant impact on susceptibility to infectious pancreatic necrosis. In the present study, additional genetic markers were mapped using the same strain in order to increase QTL resolution. A total of 199 individuals were screened at 226 marker loci (106 microsatellites, 116 amplified fragment length polymorphism markers, classical major histocompatibility complex molecules [MHC class I, nonclassical MHC class I, MHC class II] and tyrosinase) and the QTL were determined by single-point and interval mappings. Seven QTL were distinguished in the linkage groups RT-11, 12, 17, 23, 26, 29 and 31, in addition to the two already known in RT-3 and 22. The major QTL were those in RT-3, 12 and 22; whereas the classical MHC class I locus Onmy-UBA and the MHC class II locus Onmy-DAB were not associated with any of the QTL, a nonclassical MHC class I region with Onmy-UCA, Onmy-UDA, and Onmy-UEA mapped to the major QTL on RT-3.
AB - Previous typing of microsatellite markers in a BC1F1 backcross rainbow trout Oncorhynchus mykiss revealed two quantitative trait loci (QTL) with a significant impact on susceptibility to infectious pancreatic necrosis. In the present study, additional genetic markers were mapped using the same strain in order to increase QTL resolution. A total of 199 individuals were screened at 226 marker loci (106 microsatellites, 116 amplified fragment length polymorphism markers, classical major histocompatibility complex molecules [MHC class I, nonclassical MHC class I, MHC class II] and tyrosinase) and the QTL were determined by single-point and interval mappings. Seven QTL were distinguished in the linkage groups RT-11, 12, 17, 23, 26, 29 and 31, in addition to the two already known in RT-3 and 22. The major QTL were those in RT-3, 12 and 22; whereas the classical MHC class I locus Onmy-UBA and the MHC class II locus Onmy-DAB were not associated with any of the QTL, a nonclassical MHC class I region with Onmy-UCA, Onmy-UDA, and Onmy-UEA mapped to the major QTL on RT-3.
UR - http://www.scopus.com/inward/record.url?scp=36148967247&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36148967247&partnerID=8YFLogxK
U2 - 10.3147/jsfp.42.131
DO - 10.3147/jsfp.42.131
M3 - Article
AN - SCOPUS:36148967247
SN - 0388-788X
VL - 42
SP - 131
EP - 140
JO - Fish Pathology
JF - Fish Pathology
IS - 3
ER -