Identification of AF-6 and Canoe as putative targets for Ras

Masamitsu Kuriyama, Naozumi Harada, Shinya Kuroda, Takaharu Yamamoto, Masato Nakafuku, Akihiro Iwamatsu, Daisuke Yamamoto, Raj Prasad, Carlo Croce, Eli Canaani, Kozo Kaibuchi

Research output: Contribution to journalArticlepeer-review

187 Citations (Scopus)


Ras (Ha-Ras, Ki-Ras, N-Ras) is implicated in the regulation of various cell functions such as gene expression and cell proliferation downstream from specific extracellular signals. Here, we partially purified a Ras-interacting protein with molecular mass of about 180 kDa (p180) from bovine brain membrane extract by glutathione S-transferase (GST)-Ha-Ras affinity column chromatography. This protein bound to the GTPγS (guanosine 5'-(3-O- thio)triphosphate, a nonhydrolyzable GTP analog)-GST-Ha-Ras affinity column but not to those containing GDP·GST-Ha-Ras or GTPγS·GST-Ha-Ras with a mutation in the effector domain (Ha-Ras(A38)). The amino acid sequences of the peptides derived from p180 were almost identical to those of human AF-6 that is identified as the fusion partner of the ALL-1 protein. The ALL-1/AF- 6 chimeric protein is the critical product of the t (6:11) abnormality associated with some human leukemia. AF-6 has a GLGF/Dlg homology repeat (DHR) motif and shows a high degree of sequence similarity with Drosophila Canoe, which is assumed to function downstream from Notch in a common developmental pathway. The recombinant N-terminal domain of AF-6 and Canoe specifically interacted with GTPγS·GST-Ha-Ras. The known Ras target c-Raf- 1 inhibited the interaction of AF-6 with GTPγS·GST-Ha-Ras. These results indicate that AF-6 and Canoe are putative targets for Ras.

Original languageEnglish
Pages (from-to)607-610
Number of pages4
JournalJournal of Biological Chemistry
Issue number2
Publication statusPublished - 12-01-1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Identification of AF-6 and Canoe as putative targets for Ras'. Together they form a unique fingerprint.

Cite this