TY - JOUR
T1 - Identification of cell surface marker candidates on SV-T2 cells using DNA microarray on DLC-coated glass
AU - Tuoya,
AU - Hirayama, Koichi
AU - Nagaoka, Tadahiro
AU - Yu, Dongwei
AU - Fukuda, Takayuki
AU - Tada, Hiroko
AU - Yamada, Hidenori
AU - Seno, Masaharu
N1 - Funding Information:
The authors thank Dr. Michifumi Tanga and Mr. Masayuki Kita for their continuous encouragement throughout the study. This study was supported by a Grant-in-Aid of the consortium for R&D projects for regional revitalization from METI.
PY - 2005/8/19
Y1 - 2005/8/19
N2 - We analyzed gene expression profiles of normal mouse fibroblast BALB/c 3T3 cells and its SV40 transformant SV-T2 cells using our originally developed cell surface marker DNA microarray, which is prepared on a diamond-like carbon-coated glass. As a result, CD62L and IL-6 receptor α gene expressions were upregulated in SV-T2 and were thought to be candidates for cell surface markers of the cells. The result of microarray analysis was validated by real-time quantitative PCR, immunohistochemistry and biological assays. These data show that our cell surface marker DNA microarray should be useful in finding the candidates of cell type-specific surface markers.
AB - We analyzed gene expression profiles of normal mouse fibroblast BALB/c 3T3 cells and its SV40 transformant SV-T2 cells using our originally developed cell surface marker DNA microarray, which is prepared on a diamond-like carbon-coated glass. As a result, CD62L and IL-6 receptor α gene expressions were upregulated in SV-T2 and were thought to be candidates for cell surface markers of the cells. The result of microarray analysis was validated by real-time quantitative PCR, immunohistochemistry and biological assays. These data show that our cell surface marker DNA microarray should be useful in finding the candidates of cell type-specific surface markers.
UR - http://www.scopus.com/inward/record.url?scp=22144471936&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=22144471936&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2005.06.083
DO - 10.1016/j.bbrc.2005.06.083
M3 - Article
C2 - 16108093
AN - SCOPUS:22144471936
SN - 0006-291X
VL - 334
SP - 263
EP - 268
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -