Identification of cell surface marker candidates on SV-T2 cells using DNA microarray on DLC-coated glass

Tuoya; Koichi Hirayama; Tadahiro Nagaoka; Dongwei Yu; Takayuki Fukuda; Hiroko Tada; Hidenori Yamada; Masaharu Seno

doi:10.1016/j.bbrc.2005.06.083

Identification of cell surface marker candidates on SV-T2 cells using DNA microarray on DLC-coated glass

Tuoya
, Koichi Hirayama
, Tadahiro Nagaoka
, Dongwei Yu
, Takayuki Fukuda
, Hiroko Tada
, Hidenori Yamada
, Masaharu Seno

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

We analyzed gene expression profiles of normal mouse fibroblast BALB/c 3T3 cells and its SV40 transformant SV-T2 cells using our originally developed cell surface marker DNA microarray, which is prepared on a diamond-like carbon-coated glass. As a result, CD62L and IL-6 receptor α gene expressions were upregulated in SV-T2 and were thought to be candidates for cell surface markers of the cells. The result of microarray analysis was validated by real-time quantitative PCR, immunohistochemistry and biological assays. These data show that our cell surface marker DNA microarray should be useful in finding the candidates of cell type-specific surface markers.

Original language	English
Pages (from-to)	263-268
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	334
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2005.06.083
Publication status	Published - 19-08-2005
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2005.06.083

Cite this

@article{6f7d9a4df158476c99697dced641c869,

title = "Identification of cell surface marker candidates on SV-T2 cells using DNA microarray on DLC-coated glass",

abstract = "We analyzed gene expression profiles of normal mouse fibroblast BALB/c 3T3 cells and its SV40 transformant SV-T2 cells using our originally developed cell surface marker DNA microarray, which is prepared on a diamond-like carbon-coated glass. As a result, CD62L and IL-6 receptor α gene expressions were upregulated in SV-T2 and were thought to be candidates for cell surface markers of the cells. The result of microarray analysis was validated by real-time quantitative PCR, immunohistochemistry and biological assays. These data show that our cell surface marker DNA microarray should be useful in finding the candidates of cell type-specific surface markers.",

author = "Tuoya and Koichi Hirayama and Tadahiro Nagaoka and Dongwei Yu and Takayuki Fukuda and Hiroko Tada and Hidenori Yamada and Masaharu Seno",

note = "Funding Information: The authors thank Dr. Michifumi Tanga and Mr. Masayuki Kita for their continuous encouragement throughout the study. This study was supported by a Grant-in-Aid of the consortium for R\&D projects for regional revitalization from METI.",

year = "2005",

month = aug,

day = "19",

doi = "10.1016/j.bbrc.2005.06.083",

language = "English",

volume = "334",

pages = "263--268",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Identification of cell surface marker candidates on SV-T2 cells using DNA microarray on DLC-coated glass

AU - Tuoya,

AU - Hirayama, Koichi

AU - Nagaoka, Tadahiro

AU - Yu, Dongwei

AU - Fukuda, Takayuki

AU - Tada, Hiroko

AU - Yamada, Hidenori

AU - Seno, Masaharu

N1 - Funding Information: The authors thank Dr. Michifumi Tanga and Mr. Masayuki Kita for their continuous encouragement throughout the study. This study was supported by a Grant-in-Aid of the consortium for R&D projects for regional revitalization from METI.

PY - 2005/8/19

Y1 - 2005/8/19

N2 - We analyzed gene expression profiles of normal mouse fibroblast BALB/c 3T3 cells and its SV40 transformant SV-T2 cells using our originally developed cell surface marker DNA microarray, which is prepared on a diamond-like carbon-coated glass. As a result, CD62L and IL-6 receptor α gene expressions were upregulated in SV-T2 and were thought to be candidates for cell surface markers of the cells. The result of microarray analysis was validated by real-time quantitative PCR, immunohistochemistry and biological assays. These data show that our cell surface marker DNA microarray should be useful in finding the candidates of cell type-specific surface markers.

AB - We analyzed gene expression profiles of normal mouse fibroblast BALB/c 3T3 cells and its SV40 transformant SV-T2 cells using our originally developed cell surface marker DNA microarray, which is prepared on a diamond-like carbon-coated glass. As a result, CD62L and IL-6 receptor α gene expressions were upregulated in SV-T2 and were thought to be candidates for cell surface markers of the cells. The result of microarray analysis was validated by real-time quantitative PCR, immunohistochemistry and biological assays. These data show that our cell surface marker DNA microarray should be useful in finding the candidates of cell type-specific surface markers.

UR - https://www.scopus.com/pages/publications/22144471936

UR - https://www.scopus.com/pages/publications/22144471936#tab=citedBy

U2 - 10.1016/j.bbrc.2005.06.083

DO - 10.1016/j.bbrc.2005.06.083

M3 - Article

C2 - 16108093

AN - SCOPUS:22144471936

SN - 0006-291X

VL - 334

SP - 263

EP - 268

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Identification of cell surface marker candidates on SV-T2 cells using DNA microarray on DLC-coated glass

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this