Identification of cis- and trans-acting factors involved in the localization of MALAT-1 noncoding RNA to nuclear speckles

Ryu Miyagawa, Keiko Tano, R. I.E. Mizuno, Y. O. Nakamura, Kenichi Ijiri, Randeep Rakwal, Junko Shibato, Yoshinori Masuo, Akira Maeda, Tetsuro Hirose, Nobuyoshi Akimitsu

Research output: Contribution to journalArticle

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Abstract

MALAT-1 noncoding RNA is localized to nuclear speckles despite its mRNA-like characteristics. Here, we report the identification of several key factors that promote the localization of MALAT-1 to nuclear speckles and also provide evidence that MALAT-1 is involved in the regulation of gene expression. Heterokaryon assays revealed that MALAT-1 does not shuttle between the nucleus and cytoplasm. RNAi-mediated repression of the nuclear speckle proteins, RNPS1, SRm160, or IBP160, which are well-known mRNA processing factors, resulted in the diffusion of MALAT-1 to the nucleoplasm. We demonstrated that MALAT-1 contains two distinct elements directing transcripts to nuclear speckles, which were also capable of binding to RNPS1 in vitro. Depletion of MALAT-1 represses the expression of several genes. Taken together, our results suggest that RNPS1, SRm160, and IBP160 contribute to the localization of MALAT-1 to nuclear speckles, where MALAT-1 could be involved in regulating gene expression.

Original languageEnglish
Pages (from-to)738-751
Number of pages14
JournalRNA
Volume18
Issue number4
DOIs
Publication statusPublished - 01-04-2012

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Untranslated RNA
Trans-Activators
Gene Expression
Messenger RNA
Gene Expression Regulation
Nuclear Proteins
RNA Interference
Cytoplasm
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

Miyagawa, R., Tano, K., Mizuno, R. I. E., Nakamura, Y. O., Ijiri, K., Rakwal, R., ... Akimitsu, N. (2012). Identification of cis- and trans-acting factors involved in the localization of MALAT-1 noncoding RNA to nuclear speckles. RNA, 18(4), 738-751. https://doi.org/10.1261/rna.028639.111
Miyagawa, Ryu ; Tano, Keiko ; Mizuno, R. I.E. ; Nakamura, Y. O. ; Ijiri, Kenichi ; Rakwal, Randeep ; Shibato, Junko ; Masuo, Yoshinori ; Maeda, Akira ; Hirose, Tetsuro ; Akimitsu, Nobuyoshi. / Identification of cis- and trans-acting factors involved in the localization of MALAT-1 noncoding RNA to nuclear speckles. In: RNA. 2012 ; Vol. 18, No. 4. pp. 738-751.
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Miyagawa, R, Tano, K, Mizuno, RIE, Nakamura, YO, Ijiri, K, Rakwal, R, Shibato, J, Masuo, Y, Maeda, A, Hirose, T & Akimitsu, N 2012, 'Identification of cis- and trans-acting factors involved in the localization of MALAT-1 noncoding RNA to nuclear speckles', RNA, vol. 18, no. 4, pp. 738-751. https://doi.org/10.1261/rna.028639.111

Identification of cis- and trans-acting factors involved in the localization of MALAT-1 noncoding RNA to nuclear speckles. / Miyagawa, Ryu; Tano, Keiko; Mizuno, R. I.E.; Nakamura, Y. O.; Ijiri, Kenichi; Rakwal, Randeep; Shibato, Junko; Masuo, Yoshinori; Maeda, Akira; Hirose, Tetsuro; Akimitsu, Nobuyoshi.

In: RNA, Vol. 18, No. 4, 01.04.2012, p. 738-751.

Research output: Contribution to journalArticle

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AU - Nakamura, Y. O.

AU - Ijiri, Kenichi

AU - Rakwal, Randeep

AU - Shibato, Junko

AU - Masuo, Yoshinori

AU - Maeda, Akira

AU - Hirose, Tetsuro

AU - Akimitsu, Nobuyoshi

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