Identification of DNA-dependent protein kinase catalytic subunit as a novel interaction partner of lymphocyte enhancer factor 1

Atsushi Shimomura, Akihiko Takasaki, Ryuji Nomura, Nobuhiro Hayashi, Takao Senda

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Lymphocyte enhancer factor 1 (LEF1), a member of the LEF/T-cell-specific factor (TCF) family of the high mobility group domain transcription factors, acts downstream in canonical Wnt signaling. Aberrant transactivation of LEF1 contributes to the tumorigenesis of colonic neoplasms, sebaceous skin tumors, and lymphoblastic leukemia. LEF1-associated proteins are crucial for regulating its transcriptional activity. In this study, glutathione-S-transferase pull-down assay and mass spectrometry enabled identification of the DNA-dependent protein kinase catalytic subunit (DNA-PKcs) as a novel interaction partner for LEF1. The interaction between LEF1 and DNA-PKcs was confirmed using in vivo co-immunoprecipitation. Furthermore, double immunofluorescence observations showed that LEF1 and DNA-PKcs colocalized in the nuclei of colon adenocarcinoma cell lines. Identification of the interaction between LEF1 and DNA-PKcs may provide clues for a novel therapy for cancer treatment as well as for understanding LEF1-mediated transcriptional regulation.

Original languageEnglish
Pages (from-to)14-19
Number of pages6
JournalMedical Molecular Morphology
Volume46
Issue number1
DOIs
Publication statusPublished - 16-01-2013

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DNA-Activated Protein Kinase
Catalytic Domain
Lymphocytes
TCF Transcription Factors
Glutathione Transferase
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Immunoprecipitation
Colonic Neoplasms
Transcriptional Activation
Fluorescent Antibody Technique
Mass Spectrometry
Neoplasms
Colon
Carcinogenesis
Adenocarcinoma
Transcription Factors
Cell Line
Skin

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology

Cite this

Shimomura, Atsushi ; Takasaki, Akihiko ; Nomura, Ryuji ; Hayashi, Nobuhiro ; Senda, Takao. / Identification of DNA-dependent protein kinase catalytic subunit as a novel interaction partner of lymphocyte enhancer factor 1. In: Medical Molecular Morphology. 2013 ; Vol. 46, No. 1. pp. 14-19.
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Identification of DNA-dependent protein kinase catalytic subunit as a novel interaction partner of lymphocyte enhancer factor 1. / Shimomura, Atsushi; Takasaki, Akihiko; Nomura, Ryuji; Hayashi, Nobuhiro; Senda, Takao.

In: Medical Molecular Morphology, Vol. 46, No. 1, 16.01.2013, p. 14-19.

Research output: Contribution to journalArticle

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