Identification of ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3) as a regulator of N-acetylglucosaminyltransferase GnT-IX (GnT-Vb)

Hiroaki Korekane, Jong Yi Park, Akio Matsumoto, Kazuki Nakajima, Shinji Takamatsu, Kazuaki Ohtsubo, Yasuhide Miyamoto, Shinya Hanashima, Kenji Kanekiyo, Shinobu Kitazume, Yoshiki Yamaguchi, Ichiro Matsuo, Naoyuki Taniguchi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background: The regulatory mechanisms of glycosyltransferase activity are still poorly understood. Results: ENPP3 was identified as an inhibitory factor for N-acetylglucosaminyltransferase GnT-IX (GnT-Vb) in Neuro2a cells. The underlying basis for this inhibition is the ENPP3-catalyzed hydrolysis of the nucleotide sugar donor substrate. Conclusion: ENPP3 is a regulator of glycosyltransferase activity. Significance: A novel regulatory system for the cellular glycosylation process is proposed.

Original languageEnglish
Pages (from-to)27912-27926
Number of pages15
JournalJournal of Biological Chemistry
Volume288
Issue number39
DOIs
Publication statusPublished - 27-09-2013

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Pyrophosphatases
Phosphoric Diester Hydrolases
Glycosyltransferases
Glycosylation
Sugars
Hydrolysis
Nucleotides
Substrates
N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Korekane, Hiroaki ; Park, Jong Yi ; Matsumoto, Akio ; Nakajima, Kazuki ; Takamatsu, Shinji ; Ohtsubo, Kazuaki ; Miyamoto, Yasuhide ; Hanashima, Shinya ; Kanekiyo, Kenji ; Kitazume, Shinobu ; Yamaguchi, Yoshiki ; Matsuo, Ichiro ; Taniguchi, Naoyuki. / Identification of ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3) as a regulator of N-acetylglucosaminyltransferase GnT-IX (GnT-Vb). In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 39. pp. 27912-27926.
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abstract = "Background: The regulatory mechanisms of glycosyltransferase activity are still poorly understood. Results: ENPP3 was identified as an inhibitory factor for N-acetylglucosaminyltransferase GnT-IX (GnT-Vb) in Neuro2a cells. The underlying basis for this inhibition is the ENPP3-catalyzed hydrolysis of the nucleotide sugar donor substrate. Conclusion: ENPP3 is a regulator of glycosyltransferase activity. Significance: A novel regulatory system for the cellular glycosylation process is proposed.",
author = "Hiroaki Korekane and Park, {Jong Yi} and Akio Matsumoto and Kazuki Nakajima and Shinji Takamatsu and Kazuaki Ohtsubo and Yasuhide Miyamoto and Shinya Hanashima and Kenji Kanekiyo and Shinobu Kitazume and Yoshiki Yamaguchi and Ichiro Matsuo and Naoyuki Taniguchi",
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Korekane, H, Park, JY, Matsumoto, A, Nakajima, K, Takamatsu, S, Ohtsubo, K, Miyamoto, Y, Hanashima, S, Kanekiyo, K, Kitazume, S, Yamaguchi, Y, Matsuo, I & Taniguchi, N 2013, 'Identification of ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3) as a regulator of N-acetylglucosaminyltransferase GnT-IX (GnT-Vb)', Journal of Biological Chemistry, vol. 288, no. 39, pp. 27912-27926. https://doi.org/10.1074/jbc.M113.474304

Identification of ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3) as a regulator of N-acetylglucosaminyltransferase GnT-IX (GnT-Vb). / Korekane, Hiroaki; Park, Jong Yi; Matsumoto, Akio; Nakajima, Kazuki; Takamatsu, Shinji; Ohtsubo, Kazuaki; Miyamoto, Yasuhide; Hanashima, Shinya; Kanekiyo, Kenji; Kitazume, Shinobu; Yamaguchi, Yoshiki; Matsuo, Ichiro; Taniguchi, Naoyuki.

In: Journal of Biological Chemistry, Vol. 288, No. 39, 27.09.2013, p. 27912-27926.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Identification of ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3) as a regulator of N-acetylglucosaminyltransferase GnT-IX (GnT-Vb)

AU - Korekane, Hiroaki

AU - Park, Jong Yi

AU - Matsumoto, Akio

AU - Nakajima, Kazuki

AU - Takamatsu, Shinji

AU - Ohtsubo, Kazuaki

AU - Miyamoto, Yasuhide

AU - Hanashima, Shinya

AU - Kanekiyo, Kenji

AU - Kitazume, Shinobu

AU - Yamaguchi, Yoshiki

AU - Matsuo, Ichiro

AU - Taniguchi, Naoyuki

PY - 2013/9/27

Y1 - 2013/9/27

N2 - Background: The regulatory mechanisms of glycosyltransferase activity are still poorly understood. Results: ENPP3 was identified as an inhibitory factor for N-acetylglucosaminyltransferase GnT-IX (GnT-Vb) in Neuro2a cells. The underlying basis for this inhibition is the ENPP3-catalyzed hydrolysis of the nucleotide sugar donor substrate. Conclusion: ENPP3 is a regulator of glycosyltransferase activity. Significance: A novel regulatory system for the cellular glycosylation process is proposed.

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