Identification of ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3) as a regulator of N-acetylglucosaminyltransferase GnT-IX (GnT-Vb)

Hiroaki Korekane, Jong Yi Park, Akio Matsumoto, Kazuki Nakajima, Shinji Takamatsu, Kazuaki Ohtsubo, Yasuhide Miyamoto, Shinya Hanashima, Kenji Kanekiyo, Shinobu Kitazume, Yoshiki Yamaguchi, Ichiro Matsuo, Naoyuki Taniguchi

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Abstract

Background: The regulatory mechanisms of glycosyltransferase activity are still poorly understood. Results: ENPP3 was identified as an inhibitory factor for N-acetylglucosaminyltransferase GnT-IX (GnT-Vb) in Neuro2a cells. The underlying basis for this inhibition is the ENPP3-catalyzed hydrolysis of the nucleotide sugar donor substrate. Conclusion: ENPP3 is a regulator of glycosyltransferase activity. Significance: A novel regulatory system for the cellular glycosylation process is proposed.

Original languageEnglish
Pages (from-to)27912-27926
Number of pages15
JournalJournal of Biological Chemistry
Volume288
Issue number39
DOIs
Publication statusPublished - 27-09-2013

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Korekane, H., Park, J. Y., Matsumoto, A., Nakajima, K., Takamatsu, S., Ohtsubo, K., Miyamoto, Y., Hanashima, S., Kanekiyo, K., Kitazume, S., Yamaguchi, Y., Matsuo, I., & Taniguchi, N. (2013). Identification of ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3) as a regulator of N-acetylglucosaminyltransferase GnT-IX (GnT-Vb). Journal of Biological Chemistry, 288(39), 27912-27926. https://doi.org/10.1074/jbc.M113.474304