Identification of protein kinase substrates by the kinase-interacting substrate screening (KISS) approach

Mutsuki Amano, Tomoki Nishioka, Yoshimitsu Yura, Kozo Kaibuchi

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Identifying the substrates of protein kinases to understand their modes of action has been undertaken by various approaches and remains an ongoing challenge. Phosphoproteomic technologies have accelerated the accumulation of data concerning protein phosphorylation and have uncovered vast numbers of phosphorylation sites in vivo. In this unit, a novel in vitro screening approach for protein kinase substrates is presented, based on protein-protein interaction and mass spectrometry-based phosphoproteomic technology.

Original languageEnglish
Pages (from-to)14.16.1-14.16.12.
JournalCurrent Protocols in Cell Biology
Volume2016
DOIs
Publication statusPublished - 2016
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cell Biology

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