Identification of sonic hedgehog-responsive genes using cDNA microarray

Masaki Kato, Naohiko Seki, Sumio Sugano, Katsuyuki Hashimoto, Yasuhiko Masuho, Masa aki Muramatsu, Kozo Kaibuchi, Masato Nakafuku

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)


Sonic hedgehog (Shh) is a secreted signaling protein that plays important roles in a variety of developmental processes and also in pathogenesis of some human cancers and congenital diseases. Molecules that function downstream of Shh, however, still remain elusive. Here we searched for Shh-responsive genes by using an in-house cDNA microarray. Two genes were newly identified to be Shh responsive in neuroepithelial cell line MNS-70: the metal-binding protein Ceruloplasmin (Cp) and the serine protease inhibitor inter-α-trypsine inhibitor heavy chain H3 (ITIH3). In MNS-70 cells, expression of ITIH3 was regulated by Gli zinc-finger transcription factors downstream of Shh, whereas Cp appeared to be regulated by Gli-independent pathways. Cp mRNA was detected in the developing mouse brain, where its expression domain was closely adjacent to that of Shh. These results demonstrate that microarray technology provides a useful tool for studying expression of developmentally regulated genes.

Original languageEnglish
Pages (from-to)472-478
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 30-11-2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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