Identification of the ESKAPE pathogens by mass spectrometric analysis of microbial membrane glycolipids

Lisa M. Leung, William E. Fondrie, Yohei Doi, J. Kristie Johnson, Dudley K. Strickland, Robert K. Ernst, David R. Goodlett

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Rapid diagnostics that enable identification of infectious agents improve patient outcomes, antimicrobial stewardship, and length of hospital stay. Current methods for pathogen detection in the clinical laboratory include biological culture, nucleic acid amplification, ribosomal protein characterization, and genome sequencing. Pathogen identification from single colonies by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis of high abundance proteins is gaining popularity in clinical laboratories. Here, we present a novel and complementary approach that utilizes essential microbial glycolipids as chemical fingerprints for identification of individual bacterial species. Gram-positive and negative bacterial glycolipids were extracted using a single optimized protocol. Extracts of the clinically significant ESKAPE pathogens: E nterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp. were analyzed by MALDI-TOF-MS in negative ion mode to obtain glycolipid mass spectra. A library of glycolipid mass spectra from 50 microbial entries was developed that allowed bacterial speciation of the ESKAPE pathogens, as well as identification of pathogens directly from blood bottles without culture on solid medium and determination of antimicrobial peptide resistance. These results demonstrate that bacterial glycolipid mass spectra represent chemical barcodes that identify pathogens, potentially providing a useful alternative to existing diagnostics.

Original languageEnglish
Article number6403
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 01-12-2017
Externally publishedYes

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Glycolipids
Pathogens
Membranes
Clinical laboratories
Ionization
Mass spectrometry
Desorption
Lasers
Ribosomal Proteins
Bottles
Laser modes
Nucleic Acids
Amplification
Blood
Negative ions
Genes
Peptides
Proteins

All Science Journal Classification (ASJC) codes

  • General

Cite this

Leung, L. M., Fondrie, W. E., Doi, Y., Johnson, J. K., Strickland, D. K., Ernst, R. K., & Goodlett, D. R. (2017). Identification of the ESKAPE pathogens by mass spectrometric analysis of microbial membrane glycolipids. Scientific Reports, 7(1), [6403]. https://doi.org/10.1038/s41598-017-04793-4
Leung, Lisa M. ; Fondrie, William E. ; Doi, Yohei ; Johnson, J. Kristie ; Strickland, Dudley K. ; Ernst, Robert K. ; Goodlett, David R. / Identification of the ESKAPE pathogens by mass spectrometric analysis of microbial membrane glycolipids. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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Identification of the ESKAPE pathogens by mass spectrometric analysis of microbial membrane glycolipids. / Leung, Lisa M.; Fondrie, William E.; Doi, Yohei; Johnson, J. Kristie; Strickland, Dudley K.; Ernst, Robert K.; Goodlett, David R.

In: Scientific Reports, Vol. 7, No. 1, 6403, 01.12.2017.

Research output: Contribution to journalArticle

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