Identification of the indoleamine 2,3-dioxygenase nucleotide sequence in a rat liver transplant model

Tair Long Pan, Chen Lung Lin, Chao Long Chen, Yu Chun Lin, Satoshi Gojo, Tzong Hsien Lee, Yu Hsueh Wang, Roger Lord, Chia Yun Lai, Li Weng Tsu, Hui Peng Tseng, Mei Ling Wu, Yukio Iwashita, Seigo Kitano, Kuei Chen Chiang, Takashi Hashimoto, Atsushi Sugioka, Shigeru Goto

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11 Citations (Scopus)

Abstract

A tryptophan catabolizer, indoleamine 2,3-dioxygenase (IDO) is highly expressed in the placenta and plays an essential role in maternal tolerance. Recent data have shown that the administration of an IDO inhibitor blocked not only maternal tolerance but also liver allograft tolerance. However, little is known about the induction of IDO in liver allografts, although a gene specific for tryptophan 2,3-dioxygenase (TDO) is believed to be expressed in the liver. In the present study, we investigated whether IDO is induced in liver allografts. Synthetic oligonucleotide primers based on the mouse IDO cDNA sequence were used to amplify RNA derived from livers of donor, syngeneic or allogeneic OLT rats. RNA encoding IDO was induced in the rat allogeneic liver after orthotopic liver transplantation (OLT), but not in syngeneic OLT. The rat nucleotide sequence of the RT-PCR products obtained from OLT livers revealed identities of 89% homology to the mouse IDO and of 68% to the human IDO. This study demonstrated the presence of RNA encoding IDO in allogeneic OLT livers, which may be involved in the immune response after liver transplantation.

Original languageEnglish
Pages (from-to)189-194
Number of pages6
JournalTransplant Immunology
Volume8
Issue number3
DOIs
Publication statusPublished - 2000

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Transplantation

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    Pan, T. L., Lin, C. L., Chen, C. L., Lin, Y. C., Gojo, S., Lee, T. H., Wang, Y. H., Lord, R., Lai, C. Y., Tsu, L. W., Tseng, H. P., Wu, M. L., Iwashita, Y., Kitano, S., Chiang, K. C., Hashimoto, T., Sugioka, A., & Goto, S. (2000). Identification of the indoleamine 2,3-dioxygenase nucleotide sequence in a rat liver transplant model. Transplant Immunology, 8(3), 189-194. https://doi.org/10.1016/S0966-3274(00)00024-1