IgE and IgG4 autoantibodies against DFS70/LEDGF in atopic dermatitis

Kanako Watanabe, Yoshinao Muro, Kazumitsu Sugiura, Yasushi Tomita

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background. DFS70/LEDGF is a known survival factor, growth factor, and HIV-1 transporter. It is also an autoantigen recognized by IgG autoantibodies (aAbs) in patients with atopic dermatitis (AD). In the past few decades, various IgE aAbs against self-proteins have been identified in AD. Although DFS70 is considered to be one of these proteins, to date, there has not been a detailed analysis of the significance of this putative autoantigen.Objective and methods. Using recombinant protein that was generated using a baculovirus and insect cell system, we established an ELISA that can measure IgE-anti-DFS70 aAbs and IgG4-anti-DFS70 aAbs. Sera obtained from 61 AD patients and 20 healthy donors were analyzed using the ELISA.Results. Nine of 61 AD patients were determined to be IgE-anti-DFS70 aAb-positive. IgG4-anti-DFS70 aAbs were detected in nine of 61 patients. The serum thymus and activation-regulated chemokine concentrations were significantly higher in the group that was positive for both aAbs compared to the aAb-negative group, although the total serum IgE levels were not significantly different between these groups.Conclusion. We conclude that serum IgE-anti-DFS70 aAbs and IgG4-anti-DFS70 aAbs may be related to the severity of AD.

Original languageEnglish
Pages (from-to)511-519
Number of pages9
JournalAutoimmunity
Volume44
Issue number6
DOIs
Publication statusPublished - 01-09-2011

Fingerprint

Atopic Dermatitis
Autoantibodies
Immunoglobulin E
Immunoglobulin G
Autoantigens
Serum
Chemokine CCL17
Enzyme-Linked Immunosorbent Assay
lens epithelium-derived growth factor
Baculoviridae
Recombinant Proteins
Insects
HIV-1
Intercellular Signaling Peptides and Proteins
Proteins
Tissue Donors
Survival
anti-IgE antibodies

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Watanabe, Kanako ; Muro, Yoshinao ; Sugiura, Kazumitsu ; Tomita, Yasushi. / IgE and IgG4 autoantibodies against DFS70/LEDGF in atopic dermatitis. In: Autoimmunity. 2011 ; Vol. 44, No. 6. pp. 511-519.
@article{206cfd598fb34807838dd5eeec8a4cd8,
title = "IgE and IgG4 autoantibodies against DFS70/LEDGF in atopic dermatitis",
abstract = "Background. DFS70/LEDGF is a known survival factor, growth factor, and HIV-1 transporter. It is also an autoantigen recognized by IgG autoantibodies (aAbs) in patients with atopic dermatitis (AD). In the past few decades, various IgE aAbs against self-proteins have been identified in AD. Although DFS70 is considered to be one of these proteins, to date, there has not been a detailed analysis of the significance of this putative autoantigen.Objective and methods. Using recombinant protein that was generated using a baculovirus and insect cell system, we established an ELISA that can measure IgE-anti-DFS70 aAbs and IgG4-anti-DFS70 aAbs. Sera obtained from 61 AD patients and 20 healthy donors were analyzed using the ELISA.Results. Nine of 61 AD patients were determined to be IgE-anti-DFS70 aAb-positive. IgG4-anti-DFS70 aAbs were detected in nine of 61 patients. The serum thymus and activation-regulated chemokine concentrations were significantly higher in the group that was positive for both aAbs compared to the aAb-negative group, although the total serum IgE levels were not significantly different between these groups.Conclusion. We conclude that serum IgE-anti-DFS70 aAbs and IgG4-anti-DFS70 aAbs may be related to the severity of AD.",
author = "Kanako Watanabe and Yoshinao Muro and Kazumitsu Sugiura and Yasushi Tomita",
year = "2011",
month = "9",
day = "1",
doi = "10.3109/08916934.2010.549157",
language = "English",
volume = "44",
pages = "511--519",
journal = "Autoimmunity",
issn = "0891-6934",
publisher = "Informa Healthcare",
number = "6",

}

IgE and IgG4 autoantibodies against DFS70/LEDGF in atopic dermatitis. / Watanabe, Kanako; Muro, Yoshinao; Sugiura, Kazumitsu; Tomita, Yasushi.

In: Autoimmunity, Vol. 44, No. 6, 01.09.2011, p. 511-519.

Research output: Contribution to journalArticle

TY - JOUR

T1 - IgE and IgG4 autoantibodies against DFS70/LEDGF in atopic dermatitis

AU - Watanabe, Kanako

AU - Muro, Yoshinao

AU - Sugiura, Kazumitsu

AU - Tomita, Yasushi

PY - 2011/9/1

Y1 - 2011/9/1

N2 - Background. DFS70/LEDGF is a known survival factor, growth factor, and HIV-1 transporter. It is also an autoantigen recognized by IgG autoantibodies (aAbs) in patients with atopic dermatitis (AD). In the past few decades, various IgE aAbs against self-proteins have been identified in AD. Although DFS70 is considered to be one of these proteins, to date, there has not been a detailed analysis of the significance of this putative autoantigen.Objective and methods. Using recombinant protein that was generated using a baculovirus and insect cell system, we established an ELISA that can measure IgE-anti-DFS70 aAbs and IgG4-anti-DFS70 aAbs. Sera obtained from 61 AD patients and 20 healthy donors were analyzed using the ELISA.Results. Nine of 61 AD patients were determined to be IgE-anti-DFS70 aAb-positive. IgG4-anti-DFS70 aAbs were detected in nine of 61 patients. The serum thymus and activation-regulated chemokine concentrations were significantly higher in the group that was positive for both aAbs compared to the aAb-negative group, although the total serum IgE levels were not significantly different between these groups.Conclusion. We conclude that serum IgE-anti-DFS70 aAbs and IgG4-anti-DFS70 aAbs may be related to the severity of AD.

AB - Background. DFS70/LEDGF is a known survival factor, growth factor, and HIV-1 transporter. It is also an autoantigen recognized by IgG autoantibodies (aAbs) in patients with atopic dermatitis (AD). In the past few decades, various IgE aAbs against self-proteins have been identified in AD. Although DFS70 is considered to be one of these proteins, to date, there has not been a detailed analysis of the significance of this putative autoantigen.Objective and methods. Using recombinant protein that was generated using a baculovirus and insect cell system, we established an ELISA that can measure IgE-anti-DFS70 aAbs and IgG4-anti-DFS70 aAbs. Sera obtained from 61 AD patients and 20 healthy donors were analyzed using the ELISA.Results. Nine of 61 AD patients were determined to be IgE-anti-DFS70 aAb-positive. IgG4-anti-DFS70 aAbs were detected in nine of 61 patients. The serum thymus and activation-regulated chemokine concentrations were significantly higher in the group that was positive for both aAbs compared to the aAb-negative group, although the total serum IgE levels were not significantly different between these groups.Conclusion. We conclude that serum IgE-anti-DFS70 aAbs and IgG4-anti-DFS70 aAbs may be related to the severity of AD.

UR - http://www.scopus.com/inward/record.url?scp=80051529993&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80051529993&partnerID=8YFLogxK

U2 - 10.3109/08916934.2010.549157

DO - 10.3109/08916934.2010.549157

M3 - Article

C2 - 21329475

AN - SCOPUS:80051529993

VL - 44

SP - 511

EP - 519

JO - Autoimmunity

JF - Autoimmunity

SN - 0891-6934

IS - 6

ER -