Abstract
Background. DFS70/LEDGF is a known survival factor, growth factor, and HIV-1 transporter. It is also an autoantigen recognized by IgG autoantibodies (aAbs) in patients with atopic dermatitis (AD). In the past few decades, various IgE aAbs against self-proteins have been identified in AD. Although DFS70 is considered to be one of these proteins, to date, there has not been a detailed analysis of the significance of this putative autoantigen.Objective and methods. Using recombinant protein that was generated using a baculovirus and insect cell system, we established an ELISA that can measure IgE-anti-DFS70 aAbs and IgG4-anti-DFS70 aAbs. Sera obtained from 61 AD patients and 20 healthy donors were analyzed using the ELISA.Results. Nine of 61 AD patients were determined to be IgE-anti-DFS70 aAb-positive. IgG4-anti-DFS70 aAbs were detected in nine of 61 patients. The serum thymus and activation-regulated chemokine concentrations were significantly higher in the group that was positive for both aAbs compared to the aAb-negative group, although the total serum IgE levels were not significantly different between these groups.Conclusion. We conclude that serum IgE-anti-DFS70 aAbs and IgG4-anti-DFS70 aAbs may be related to the severity of AD.
| Original language | English |
|---|---|
| Pages (from-to) | 511-519 |
| Number of pages | 9 |
| Journal | Autoimmunity |
| Volume | 44 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 01-09-2011 |
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All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Immunology
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IgE and IgG4 autoantibodies against DFS70/LEDGF in atopic dermatitis. / Watanabe, Kanako; Muro, Yoshinao; Sugiura, Kazumitsu; Tomita, Yasushi.
In: Autoimmunity, Vol. 44, No. 6, 01.09.2011, p. 511-519.Research output: Contribution to journal › Article
TY - JOUR
T1 - IgE and IgG4 autoantibodies against DFS70/LEDGF in atopic dermatitis
AU - Watanabe, Kanako
AU - Muro, Yoshinao
AU - Sugiura, Kazumitsu
AU - Tomita, Yasushi
PY - 2011/9/1
Y1 - 2011/9/1
N2 - Background. DFS70/LEDGF is a known survival factor, growth factor, and HIV-1 transporter. It is also an autoantigen recognized by IgG autoantibodies (aAbs) in patients with atopic dermatitis (AD). In the past few decades, various IgE aAbs against self-proteins have been identified in AD. Although DFS70 is considered to be one of these proteins, to date, there has not been a detailed analysis of the significance of this putative autoantigen.Objective and methods. Using recombinant protein that was generated using a baculovirus and insect cell system, we established an ELISA that can measure IgE-anti-DFS70 aAbs and IgG4-anti-DFS70 aAbs. Sera obtained from 61 AD patients and 20 healthy donors were analyzed using the ELISA.Results. Nine of 61 AD patients were determined to be IgE-anti-DFS70 aAb-positive. IgG4-anti-DFS70 aAbs were detected in nine of 61 patients. The serum thymus and activation-regulated chemokine concentrations were significantly higher in the group that was positive for both aAbs compared to the aAb-negative group, although the total serum IgE levels were not significantly different between these groups.Conclusion. We conclude that serum IgE-anti-DFS70 aAbs and IgG4-anti-DFS70 aAbs may be related to the severity of AD.
AB - Background. DFS70/LEDGF is a known survival factor, growth factor, and HIV-1 transporter. It is also an autoantigen recognized by IgG autoantibodies (aAbs) in patients with atopic dermatitis (AD). In the past few decades, various IgE aAbs against self-proteins have been identified in AD. Although DFS70 is considered to be one of these proteins, to date, there has not been a detailed analysis of the significance of this putative autoantigen.Objective and methods. Using recombinant protein that was generated using a baculovirus and insect cell system, we established an ELISA that can measure IgE-anti-DFS70 aAbs and IgG4-anti-DFS70 aAbs. Sera obtained from 61 AD patients and 20 healthy donors were analyzed using the ELISA.Results. Nine of 61 AD patients were determined to be IgE-anti-DFS70 aAb-positive. IgG4-anti-DFS70 aAbs were detected in nine of 61 patients. The serum thymus and activation-regulated chemokine concentrations were significantly higher in the group that was positive for both aAbs compared to the aAb-negative group, although the total serum IgE levels were not significantly different between these groups.Conclusion. We conclude that serum IgE-anti-DFS70 aAbs and IgG4-anti-DFS70 aAbs may be related to the severity of AD.
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U2 - 10.3109/08916934.2010.549157
DO - 10.3109/08916934.2010.549157
M3 - Article
VL - 44
SP - 511
EP - 519
JO - Autoimmunity
JF - Autoimmunity
SN - 0891-6934
IS - 6
ER -