IL-1β and TNF-α suppress apolipoprotein (APO) E secretion and APO A-I expression in HepG2 cells

Hua Song, Kuniaki Saito, Suwako Fujigaki, Akio Noma, Hiroshi Ishiguro, Toshiharu Nagatsu, Mitsuru Seishima

Research output: Contribution to journalArticle

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Abstract

The effect of cytokines on apolipoprotein E (ape E) production and secretion was investigated in a human hepatoma cell line HepG2. Incubation of HepG2 cells with interleukin 1β (IL-1β) or tumour necrosis factor α (TNF-α) for 48 h resulted in a significant dose-related decrease of apo E concentration in the culture medium, while intracellular apo E content increased without change in mRNA level. In contrast, IL-1β and TNF-α decreased both intracellular and medium apo A-I. Elution profiles of cholesterol and apolipoproteins revealed that apo E was present in apo E-rich high density lipoprotein (HDL) fraction and apo A-I was in apo E-rich HDL and small HDL fractions. IL-1β and TNF-α decreased both apo E and apo A-I in these fractions. The present results suggest that IL-1β and TNF-α suppress hepatic apo A-I expression and secretion but not expression of apo E, which could contribute to the abnormal lipid metabolism in certain cytokine-mediated inflammatory diseases.

Original languageEnglish
Pages (from-to)275-280
Number of pages6
JournalCytokine
Volume10
Issue number4
DOIs
Publication statusPublished - 01-01-1998
Externally publishedYes

Fingerprint

Apolipoprotein A-I
Hep G2 Cells
Apolipoproteins E
Interleukin-1
HDL Lipoproteins
Cytokines
Apolipoproteins
Lipid Metabolism
Culture Media
Hepatocellular Carcinoma
Tumor Necrosis Factor-alpha
Cholesterol
Cells
Cell Line
Messenger RNA
Liver

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

Cite this

Song, H., Saito, K., Fujigaki, S., Noma, A., Ishiguro, H., Nagatsu, T., & Seishima, M. (1998). IL-1β and TNF-α suppress apolipoprotein (APO) E secretion and APO A-I expression in HepG2 cells. Cytokine, 10(4), 275-280. https://doi.org/10.1006/cyto.1997.0291
Song, Hua ; Saito, Kuniaki ; Fujigaki, Suwako ; Noma, Akio ; Ishiguro, Hiroshi ; Nagatsu, Toshiharu ; Seishima, Mitsuru. / IL-1β and TNF-α suppress apolipoprotein (APO) E secretion and APO A-I expression in HepG2 cells. In: Cytokine. 1998 ; Vol. 10, No. 4. pp. 275-280.
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abstract = "The effect of cytokines on apolipoprotein E (ape E) production and secretion was investigated in a human hepatoma cell line HepG2. Incubation of HepG2 cells with interleukin 1β (IL-1β) or tumour necrosis factor α (TNF-α) for 48 h resulted in a significant dose-related decrease of apo E concentration in the culture medium, while intracellular apo E content increased without change in mRNA level. In contrast, IL-1β and TNF-α decreased both intracellular and medium apo A-I. Elution profiles of cholesterol and apolipoproteins revealed that apo E was present in apo E-rich high density lipoprotein (HDL) fraction and apo A-I was in apo E-rich HDL and small HDL fractions. IL-1β and TNF-α decreased both apo E and apo A-I in these fractions. The present results suggest that IL-1β and TNF-α suppress hepatic apo A-I expression and secretion but not expression of apo E, which could contribute to the abnormal lipid metabolism in certain cytokine-mediated inflammatory diseases.",
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Song, H, Saito, K, Fujigaki, S, Noma, A, Ishiguro, H, Nagatsu, T & Seishima, M 1998, 'IL-1β and TNF-α suppress apolipoprotein (APO) E secretion and APO A-I expression in HepG2 cells', Cytokine, vol. 10, no. 4, pp. 275-280. https://doi.org/10.1006/cyto.1997.0291

IL-1β and TNF-α suppress apolipoprotein (APO) E secretion and APO A-I expression in HepG2 cells. / Song, Hua; Saito, Kuniaki; Fujigaki, Suwako; Noma, Akio; Ishiguro, Hiroshi; Nagatsu, Toshiharu; Seishima, Mitsuru.

In: Cytokine, Vol. 10, No. 4, 01.01.1998, p. 275-280.

Research output: Contribution to journalArticle

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AU - Song, Hua

AU - Saito, Kuniaki

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AU - Noma, Akio

AU - Ishiguro, Hiroshi

AU - Nagatsu, Toshiharu

AU - Seishima, Mitsuru

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