IL-10 and RANTES are elevated in nasopharyngeal secretions of children with respiratory syncytial virus infection

Hiroki Murai, Akihiko Terada, Mihoko Mizuno, Masami Asai, Yasutaka Hirabayashi, Seiki Shimizu, Takehiro Morishita, Hiroki Kakita, Mohamed Hamed Hussein, Tetsuya Ito, Ineko Kato, Kiyofumi Asai, Hajime Togari

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Background: Respiratory syncytial virus (RSV) infection causes asthma-like symptoms in infants and young children. Although an increase in several mediators in the airway during RSV infection has been reported, the mechanisms involved in airway inflammation are not fully understood. The aim of this study was to investigate the immunological deviation associated with airway inflammation by measuring cytokine and chemokine levels in the airway during RSV infection. Methods: One hundred and ten children under 3 years of age with respiratory symptoms were enrolled in this study from November 2004 through January 2005. Nasopharyngeal secretions (NPAs) were gently aspirated and analyzed with RSV antigen, thereafter the concentrations of IL-4, IL-10, IFN-γ, and RANTES were measured using an ELISA kit. We also investigated the prognosis of each child after 1 year by reference to clinical records or by interviews and re-evaluated the cytokine and chemokine levels. Results: Of the subjects, 70 children were RSV positive and 40 were negative. Only 4 children were given a diagnosis of asthma by the pediatrician when NPAs were collected. The levels of IL-4, IL-10, and RANTES were significantly higher in the RSV-positive patients than RSV-negative patients with P values at 0.0362, 0.0007, and 0.0047, respectively. In contrast, there was no significant difference in the levels of IFN-γ. Furthermore, there was a significant positive correlation between IL-10 and RANTES. Conclusions: The increased production of IL-4, IL-10, and RANTES in the airway may play an important role in the pathophysiological mechanisms of RSV infection.

Original languageEnglish
Pages (from-to)157-163
Number of pages7
JournalAllergology International
Volume56
Issue number2
DOIs
Publication statusPublished - 01-01-2007

Fingerprint

Respiratory Syncytial Virus Infections
Chemokine CCL5
Respiratory Syncytial Viruses
Interleukin-10
Interleukin-4
Chemokines
Asthma
Cytokines
Inflammation
Enzyme-Linked Immunosorbent Assay
Interviews
Antigens

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy

Cite this

Murai, Hiroki ; Terada, Akihiko ; Mizuno, Mihoko ; Asai, Masami ; Hirabayashi, Yasutaka ; Shimizu, Seiki ; Morishita, Takehiro ; Kakita, Hiroki ; Hussein, Mohamed Hamed ; Ito, Tetsuya ; Kato, Ineko ; Asai, Kiyofumi ; Togari, Hajime. / IL-10 and RANTES are elevated in nasopharyngeal secretions of children with respiratory syncytial virus infection. In: Allergology International. 2007 ; Vol. 56, No. 2. pp. 157-163.
@article{f713f37cad9d4634b6d4bdfc52891846,
title = "IL-10 and RANTES are elevated in nasopharyngeal secretions of children with respiratory syncytial virus infection",
abstract = "Background: Respiratory syncytial virus (RSV) infection causes asthma-like symptoms in infants and young children. Although an increase in several mediators in the airway during RSV infection has been reported, the mechanisms involved in airway inflammation are not fully understood. The aim of this study was to investigate the immunological deviation associated with airway inflammation by measuring cytokine and chemokine levels in the airway during RSV infection. Methods: One hundred and ten children under 3 years of age with respiratory symptoms were enrolled in this study from November 2004 through January 2005. Nasopharyngeal secretions (NPAs) were gently aspirated and analyzed with RSV antigen, thereafter the concentrations of IL-4, IL-10, IFN-γ, and RANTES were measured using an ELISA kit. We also investigated the prognosis of each child after 1 year by reference to clinical records or by interviews and re-evaluated the cytokine and chemokine levels. Results: Of the subjects, 70 children were RSV positive and 40 were negative. Only 4 children were given a diagnosis of asthma by the pediatrician when NPAs were collected. The levels of IL-4, IL-10, and RANTES were significantly higher in the RSV-positive patients than RSV-negative patients with P values at 0.0362, 0.0007, and 0.0047, respectively. In contrast, there was no significant difference in the levels of IFN-γ. Furthermore, there was a significant positive correlation between IL-10 and RANTES. Conclusions: The increased production of IL-4, IL-10, and RANTES in the airway may play an important role in the pathophysiological mechanisms of RSV infection.",
author = "Hiroki Murai and Akihiko Terada and Mihoko Mizuno and Masami Asai and Yasutaka Hirabayashi and Seiki Shimizu and Takehiro Morishita and Hiroki Kakita and Hussein, {Mohamed Hamed} and Tetsuya Ito and Ineko Kato and Kiyofumi Asai and Hajime Togari",
year = "2007",
month = "1",
day = "1",
doi = "10.2332/allergolint.O-06-454",
language = "English",
volume = "56",
pages = "157--163",
journal = "Allergology International",
issn = "1323-8930",
publisher = "Japanese Society of Allergology",
number = "2",

}

Murai, H, Terada, A, Mizuno, M, Asai, M, Hirabayashi, Y, Shimizu, S, Morishita, T, Kakita, H, Hussein, MH, Ito, T, Kato, I, Asai, K & Togari, H 2007, 'IL-10 and RANTES are elevated in nasopharyngeal secretions of children with respiratory syncytial virus infection', Allergology International, vol. 56, no. 2, pp. 157-163. https://doi.org/10.2332/allergolint.O-06-454

IL-10 and RANTES are elevated in nasopharyngeal secretions of children with respiratory syncytial virus infection. / Murai, Hiroki; Terada, Akihiko; Mizuno, Mihoko; Asai, Masami; Hirabayashi, Yasutaka; Shimizu, Seiki; Morishita, Takehiro; Kakita, Hiroki; Hussein, Mohamed Hamed; Ito, Tetsuya; Kato, Ineko; Asai, Kiyofumi; Togari, Hajime.

In: Allergology International, Vol. 56, No. 2, 01.01.2007, p. 157-163.

Research output: Contribution to journalArticle

TY - JOUR

T1 - IL-10 and RANTES are elevated in nasopharyngeal secretions of children with respiratory syncytial virus infection

AU - Murai, Hiroki

AU - Terada, Akihiko

AU - Mizuno, Mihoko

AU - Asai, Masami

AU - Hirabayashi, Yasutaka

AU - Shimizu, Seiki

AU - Morishita, Takehiro

AU - Kakita, Hiroki

AU - Hussein, Mohamed Hamed

AU - Ito, Tetsuya

AU - Kato, Ineko

AU - Asai, Kiyofumi

AU - Togari, Hajime

PY - 2007/1/1

Y1 - 2007/1/1

N2 - Background: Respiratory syncytial virus (RSV) infection causes asthma-like symptoms in infants and young children. Although an increase in several mediators in the airway during RSV infection has been reported, the mechanisms involved in airway inflammation are not fully understood. The aim of this study was to investigate the immunological deviation associated with airway inflammation by measuring cytokine and chemokine levels in the airway during RSV infection. Methods: One hundred and ten children under 3 years of age with respiratory symptoms were enrolled in this study from November 2004 through January 2005. Nasopharyngeal secretions (NPAs) were gently aspirated and analyzed with RSV antigen, thereafter the concentrations of IL-4, IL-10, IFN-γ, and RANTES were measured using an ELISA kit. We also investigated the prognosis of each child after 1 year by reference to clinical records or by interviews and re-evaluated the cytokine and chemokine levels. Results: Of the subjects, 70 children were RSV positive and 40 were negative. Only 4 children were given a diagnosis of asthma by the pediatrician when NPAs were collected. The levels of IL-4, IL-10, and RANTES were significantly higher in the RSV-positive patients than RSV-negative patients with P values at 0.0362, 0.0007, and 0.0047, respectively. In contrast, there was no significant difference in the levels of IFN-γ. Furthermore, there was a significant positive correlation between IL-10 and RANTES. Conclusions: The increased production of IL-4, IL-10, and RANTES in the airway may play an important role in the pathophysiological mechanisms of RSV infection.

AB - Background: Respiratory syncytial virus (RSV) infection causes asthma-like symptoms in infants and young children. Although an increase in several mediators in the airway during RSV infection has been reported, the mechanisms involved in airway inflammation are not fully understood. The aim of this study was to investigate the immunological deviation associated with airway inflammation by measuring cytokine and chemokine levels in the airway during RSV infection. Methods: One hundred and ten children under 3 years of age with respiratory symptoms were enrolled in this study from November 2004 through January 2005. Nasopharyngeal secretions (NPAs) were gently aspirated and analyzed with RSV antigen, thereafter the concentrations of IL-4, IL-10, IFN-γ, and RANTES were measured using an ELISA kit. We also investigated the prognosis of each child after 1 year by reference to clinical records or by interviews and re-evaluated the cytokine and chemokine levels. Results: Of the subjects, 70 children were RSV positive and 40 were negative. Only 4 children were given a diagnosis of asthma by the pediatrician when NPAs were collected. The levels of IL-4, IL-10, and RANTES were significantly higher in the RSV-positive patients than RSV-negative patients with P values at 0.0362, 0.0007, and 0.0047, respectively. In contrast, there was no significant difference in the levels of IFN-γ. Furthermore, there was a significant positive correlation between IL-10 and RANTES. Conclusions: The increased production of IL-4, IL-10, and RANTES in the airway may play an important role in the pathophysiological mechanisms of RSV infection.

UR - http://www.scopus.com/inward/record.url?scp=34250870157&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34250870157&partnerID=8YFLogxK

U2 - 10.2332/allergolint.O-06-454

DO - 10.2332/allergolint.O-06-454

M3 - Article

C2 - 17460443

AN - SCOPUS:34250870157

VL - 56

SP - 157

EP - 163

JO - Allergology International

JF - Allergology International

SN - 1323-8930

IS - 2

ER -