IL-33 mediates inflammatory responses in human lung tissue cells

Akiko Yagami, Kanami Orihara, Hideaki Morita, Kyoko Futamura, Noriko Hashimoto, Kenji Matsumoto, Hirohisa Saito, Akio Matsuda

Research output: Contribution to journalArticle

161 Citations (Scopus)

Abstract

IL-33 is a member of the IL-1 family and mediates its biological effects via the ST2 receptor, which is selectively expressed on Th2 cells and mast cells. Although polymorphic variation in ST2 is strongly associated with asthma, it is currently unclear whether IL-33 acts directly on lung tissue cells at sites of airway remodeling. Therefore, we aimed to identify the IL-33-responsive cells among primary human lung tissue cells. ST2 mRNA was expressed in both endothelial and epithelial cells but not in fibroblasts or smooth muscle cells. Correspondingly, IL-33 promoted IL-8 production by both endothelial and epithelial cells but not by fibroblasts or smooth muscle cells. Transfection of ST2 small interference RNA into both endothelial and epithelial cells significantly reduced the IL-33-dependent upregulation of IL-8, suggesting that IL-33-mediated responses in these cells occur via the ST2 receptor. Importantly, Th2 cytokines, such as IL-4, further enhanced ST2 expression and function in both endothelial and epithelial cells. The IL-33-mediated production of IL-8 by epithelial cells was almost completely suppressed by corticosteroid treatment. In contrast, the effect of corticosteroid treatment on the IL-33-mediated responses of endothelial cells was only partial. IL-33 induced activation of both ERK and p38 MAPK in endothelial cells but only ERK in epithelial cells. p38 MAPK was required for the IL-33-mediated responses of endothelial cells, whereas ERK was required for IL-33-mediated IL-8 production by epithelial cells. Taken together, these findings suggest that IL-33-mediated inflammatory responses of lung tissue cells may be involved in the chronic allergic inflammation of the asthmatic airway.

Original languageEnglish
Pages (from-to)5743-5750
Number of pages8
JournalJournal of Immunology
Volume185
Issue number10
DOIs
Publication statusPublished - 15-11-2010

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Lung
Endothelial Cells
Epithelial Cells
Interleukin-8
p38 Mitogen-Activated Protein Kinases
Smooth Muscle Myocytes
Interleukin-33
Adrenal Cortex Hormones
Fibroblasts
Airway Remodeling
Th2 Cells
RNA Interference
Interleukin-1
Mast Cells
Interleukin-4
Transfection
Up-Regulation
Asthma
Cytokines
Inflammation

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Yagami, A., Orihara, K., Morita, H., Futamura, K., Hashimoto, N., Matsumoto, K., ... Matsuda, A. (2010). IL-33 mediates inflammatory responses in human lung tissue cells. Journal of Immunology, 185(10), 5743-5750. https://doi.org/10.4049/jimmunol.0903818
Yagami, Akiko ; Orihara, Kanami ; Morita, Hideaki ; Futamura, Kyoko ; Hashimoto, Noriko ; Matsumoto, Kenji ; Saito, Hirohisa ; Matsuda, Akio. / IL-33 mediates inflammatory responses in human lung tissue cells. In: Journal of Immunology. 2010 ; Vol. 185, No. 10. pp. 5743-5750.
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Yagami, A, Orihara, K, Morita, H, Futamura, K, Hashimoto, N, Matsumoto, K, Saito, H & Matsuda, A 2010, 'IL-33 mediates inflammatory responses in human lung tissue cells', Journal of Immunology, vol. 185, no. 10, pp. 5743-5750. https://doi.org/10.4049/jimmunol.0903818

IL-33 mediates inflammatory responses in human lung tissue cells. / Yagami, Akiko; Orihara, Kanami; Morita, Hideaki; Futamura, Kyoko; Hashimoto, Noriko; Matsumoto, Kenji; Saito, Hirohisa; Matsuda, Akio.

In: Journal of Immunology, Vol. 185, No. 10, 15.11.2010, p. 5743-5750.

Research output: Contribution to journalArticle

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AU - Yagami, Akiko

AU - Orihara, Kanami

AU - Morita, Hideaki

AU - Futamura, Kyoko

AU - Hashimoto, Noriko

AU - Matsumoto, Kenji

AU - Saito, Hirohisa

AU - Matsuda, Akio

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Yagami A, Orihara K, Morita H, Futamura K, Hashimoto N, Matsumoto K et al. IL-33 mediates inflammatory responses in human lung tissue cells. Journal of Immunology. 2010 Nov 15;185(10):5743-5750. https://doi.org/10.4049/jimmunol.0903818