TY - JOUR
T1 - Immature multipotent hemopoietic progenitors lacking long-term bone marrow-reconstituting activity in the aorta-gonad-mesonephros region of murine day 10 fetuses
AU - Ohmura, K.
AU - Kawamoto, H.
AU - Lu, M.
AU - Ikawa, T.
AU - Ozaki, S.
AU - Nakao, K.
AU - Katsura, Y.
PY - 2001/3/1
Y1 - 2001/3/1
N2 - Previous studies indicated that multipotent progenitors exist in early fetuses that do not contain long-term reconstituting (LTR) activity. However, it remained unclear whether these multipotent progenitors are committed to the hemopoietic lineage or are immature mesodermal cells or hemangioblasts. In this study, we have succeeded in enriching the multipotent progenitors that are capable of generating myeloid, T, and B cells in the LFA-1- subpopulation of TER-119-c-kit+CD45+ cells from the aorta-gonad-mesonephros (AGM) region of day 10 fetuses. We found that these day 10 AGM LFA-1- cells do not show the LTR activity, whereas day 11 AGM LFA-1- cells do have such an activity. These results strongly suggest that multipotent progenitors lacking LTR activity emerge as CD45+ hemopoietic progenitor cells in the AGM region on the 10th day of gestation, and such p-Multi mature into hemopoietic stem cells by acquiring LTR activity.
AB - Previous studies indicated that multipotent progenitors exist in early fetuses that do not contain long-term reconstituting (LTR) activity. However, it remained unclear whether these multipotent progenitors are committed to the hemopoietic lineage or are immature mesodermal cells or hemangioblasts. In this study, we have succeeded in enriching the multipotent progenitors that are capable of generating myeloid, T, and B cells in the LFA-1- subpopulation of TER-119-c-kit+CD45+ cells from the aorta-gonad-mesonephros (AGM) region of day 10 fetuses. We found that these day 10 AGM LFA-1- cells do not show the LTR activity, whereas day 11 AGM LFA-1- cells do have such an activity. These results strongly suggest that multipotent progenitors lacking LTR activity emerge as CD45+ hemopoietic progenitor cells in the AGM region on the 10th day of gestation, and such p-Multi mature into hemopoietic stem cells by acquiring LTR activity.
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U2 - 10.4049/jimmunol.166.5.3290
DO - 10.4049/jimmunol.166.5.3290
M3 - Article
C2 - 11207284
AN - SCOPUS:0035284819
SN - 0022-1767
VL - 166
SP - 3290
EP - 3296
JO - Journal of Immunology
JF - Journal of Immunology
IS - 5
ER -