Immunocytochemical evidence that amyloid β (1-42) impairs endogenous antioxidant systems in vivo

H. C. Kim, K. Yamada, A. Nitta, A. Olariu, M. H. Tran, M. Mizuno, A. Nakajima, T. Nagai, H. Kamei, W. K. Jhoo, D. H. Im, E. J. Shin, O. P. Hjelle, O. P. Ottersen, S. C. Park, K. Kato, M. E. Mirault, Toshitaka Nabeshima

Research output: Contribution to journalArticle

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Abstract

Amyloid β, the major constituent of the senile plaques in the brains of patients with Alzheimer's disease, is cytotoxic to neurons and has a central role in the pathogenesis of the disease. We have previously demonstrated that potent antioxidants idebenone and α-tocopherol prevent learning and memory impairment in rats which received a continuous intracerebroventricular infusion of amyloid β, suggesting a role for oxidative stress in amyloid β-induced learning and memory impairment. To test the hypothesis, in the present study, we investigated alterations in the immunoreactivity of endogenous antioxidant systems such as mitochondrial Mn-superoxide dismutase, glutathione, glutathione peroxidase and glutathione-S-transferase following the continuous intracerebroventricular infusion of amyloid β for 2 weeks. The infusion of amyloid β (1-42) resulted in a significant reduction of the immunoreactivity of these antioxidant substances in such brain areas as the hippocampus, parietal cortex, piriform cortex, substantia nigra and thalamus although the same treatment with amyloid β (40-1) had little effect. The alterations induced by amyloid β (1-42) were not uniform, but rather specific for each immunoreactive substance in a brain region-dependent manner. These results demonstrate a cytological effect of oxidative stress induced by amyloid β (1-42) infusion. Furthermore, our findings may indicate a heterogeneous susceptibility to the oxidative stress produced by amyloid β.

Original languageEnglish
Pages (from-to)399-419
Number of pages21
JournalNeuroscience
Volume119
Issue number2
DOIs
Publication statusPublished - 27-06-2003

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Amyloid
Antioxidants
Intraventricular Infusions
Oxidative Stress
Brain
Learning
Parietal Lobe
Tocopherols
Amyloid Plaques
Substantia Nigra
Glutathione Peroxidase
Glutathione Transferase
Thalamus
Superoxide Dismutase
Glutathione
Hippocampus
Alzheimer Disease
Neurons

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Kim, H. C. ; Yamada, K. ; Nitta, A. ; Olariu, A. ; Tran, M. H. ; Mizuno, M. ; Nakajima, A. ; Nagai, T. ; Kamei, H. ; Jhoo, W. K. ; Im, D. H. ; Shin, E. J. ; Hjelle, O. P. ; Ottersen, O. P. ; Park, S. C. ; Kato, K. ; Mirault, M. E. ; Nabeshima, Toshitaka. / Immunocytochemical evidence that amyloid β (1-42) impairs endogenous antioxidant systems in vivo. In: Neuroscience. 2003 ; Vol. 119, No. 2. pp. 399-419.
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Kim, HC, Yamada, K, Nitta, A, Olariu, A, Tran, MH, Mizuno, M, Nakajima, A, Nagai, T, Kamei, H, Jhoo, WK, Im, DH, Shin, EJ, Hjelle, OP, Ottersen, OP, Park, SC, Kato, K, Mirault, ME & Nabeshima, T 2003, 'Immunocytochemical evidence that amyloid β (1-42) impairs endogenous antioxidant systems in vivo', Neuroscience, vol. 119, no. 2, pp. 399-419. https://doi.org/10.1016/S0306-4522(02)00993-4

Immunocytochemical evidence that amyloid β (1-42) impairs endogenous antioxidant systems in vivo. / Kim, H. C.; Yamada, K.; Nitta, A.; Olariu, A.; Tran, M. H.; Mizuno, M.; Nakajima, A.; Nagai, T.; Kamei, H.; Jhoo, W. K.; Im, D. H.; Shin, E. J.; Hjelle, O. P.; Ottersen, O. P.; Park, S. C.; Kato, K.; Mirault, M. E.; Nabeshima, Toshitaka.

In: Neuroscience, Vol. 119, No. 2, 27.06.2003, p. 399-419.

Research output: Contribution to journalArticle

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T1 - Immunocytochemical evidence that amyloid β (1-42) impairs endogenous antioxidant systems in vivo

AU - Kim, H. C.

AU - Yamada, K.

AU - Nitta, A.

AU - Olariu, A.

AU - Tran, M. H.

AU - Mizuno, M.

AU - Nakajima, A.

AU - Nagai, T.

AU - Kamei, H.

AU - Jhoo, W. K.

AU - Im, D. H.

AU - Shin, E. J.

AU - Hjelle, O. P.

AU - Ottersen, O. P.

AU - Park, S. C.

AU - Kato, K.

AU - Mirault, M. E.

AU - Nabeshima, Toshitaka

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N2 - Amyloid β, the major constituent of the senile plaques in the brains of patients with Alzheimer's disease, is cytotoxic to neurons and has a central role in the pathogenesis of the disease. We have previously demonstrated that potent antioxidants idebenone and α-tocopherol prevent learning and memory impairment in rats which received a continuous intracerebroventricular infusion of amyloid β, suggesting a role for oxidative stress in amyloid β-induced learning and memory impairment. To test the hypothesis, in the present study, we investigated alterations in the immunoreactivity of endogenous antioxidant systems such as mitochondrial Mn-superoxide dismutase, glutathione, glutathione peroxidase and glutathione-S-transferase following the continuous intracerebroventricular infusion of amyloid β for 2 weeks. The infusion of amyloid β (1-42) resulted in a significant reduction of the immunoreactivity of these antioxidant substances in such brain areas as the hippocampus, parietal cortex, piriform cortex, substantia nigra and thalamus although the same treatment with amyloid β (40-1) had little effect. The alterations induced by amyloid β (1-42) were not uniform, but rather specific for each immunoreactive substance in a brain region-dependent manner. These results demonstrate a cytological effect of oxidative stress induced by amyloid β (1-42) infusion. Furthermore, our findings may indicate a heterogeneous susceptibility to the oxidative stress produced by amyloid β.

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