Immunocytochemical features of lens after cataract tissue - Signalling molecules (growth factors, cytokines, other signalling molecules), cytoskeleton proteins, cellular and extracellular matrix proteins

Toshiro Shigemitsu, Keiji Ishiguro, Yoshito Shimizu, Masayuki Horiguchi, Masao Kasahara, Saori Arakaki

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

We examined the immunocytochemical features of after cataract tissue and cataractous lens epithelium prior to cataract surgery and at the time of surgery. Methods: This study included 23 cases. Antibodies against the following antigens were used: epidermal growth factor (EGF), EGF receptor (EGF-R), fibroblast growth factor (FGF), FGF receptor (FGF-R), transforming growth factor (TGF)-β, TGF-β receptor II (TGF-β-RII), insulin-like growth factor (IGF)II, platelet derived growth factor (PDGF)-AB, interleukin (IL)-6, IL-1 receptor II, tumor necrosis factor (TNF)-α, tissue plasminogen activator (TPA), prostaglandin (PG) E2, plasminogen activator inhibitor (PAI)-1, alpha smooth muscle actin (α-SMA), keratin, vimentin, myosin, fibronectin, laminin, types I, II, III, IV, V and VI collagen (Col), and lens epithelial cells (LECs). Results: The following substances were present in posterior and anterior capsular fibrosis of after cataract: EGF, EGF-R, FGF, FGF-R, TGF-β, TGF-β-RII, IGF-II, PDGF-AB, IL-6, IL-1RII, TNF-α, PGE2, PAI-1, α-SMA, keratin, fibronectin, laminin, Type I col, Type II to VI col and LECs. Elschnig's pearls of after cataract expressed FGF-R, TNF-α, laminin, and LECs. Soemmering's ring of after cataract tissue expressed EGF, FGF, FGF-R, IL-1RII, TPA, PA1-1, keratin and LECs. The following substances were present in cataractous lens epithelium prior to cataract surgery: EGF, EGF-R, FGF, FGF-R, TGF-β-RII, IL-1-RII, TNF-α, PAI-1, keratin, laminin and LECs. The following substances were present in the lens epithelium of anterior capsules collected at the time of cataract surgery: EGF, EGF-R, FGF, FGF-R, TGF-β, TGF-β-RII, IGF-II, IL-6, IL-1-RII, TNF-α, PGE2, keratin, fibronectin, laminin, Type IV Col and LECs. Conclusions: These results prove that many kinds of signalling molecules and proteins exist in the process of after cataract formation.

Original languageEnglish
Pages (from-to)137-144
Number of pages8
JournalInternational Ophthalmology
Volume23
Issue number3
DOIs
Publication statusPublished - 05-1999

All Science Journal Classification (ASJC) codes

  • Ophthalmology

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