Immunohistochemical analysis of apoptosis-related proteins in human embryonic and fetal pancreatic tissues

Hiroyuki Kobayashi, Ryuichiro Doi, Ryo Hosotani, Yoshiharu Miyamoto, Takatomo Koshiba, Koji Fujimoto, Jun Ida, Shoichiro Tsuji, Sanae Nakajima, Michiya Kawaguchi, Kohei Shiota, Masayuki Imamura

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background. The growth of both cancer cells and fetal tissue is rapid; however, cancer cells de-differentiate and proliferate in a disorderly manner, whereas fetal tissues differentiate and proliferate in an orderly manner. Thus, there may be both common and different factors that are involved in the process of the uncontrolled cell growth of pancreatic cancers and the development of the fetal pancreas. The common part of the mechanisms should be in the regulation of the cell cycle, resulting in rapid proliferation via such mechanisms as growth stimulation and avoidance of apoptosis. Therefore, in the current study we investigated the expression of apoptosis-related proteins in fetal pancreatic tissues. Methods. Sixteen human embryonic and fetal pancreatic tissues obtained between 6 and 32 wk of gestation were used. We immunohistochemically examined the protein expression of Bcl-2, Bcl-XL, Mcl-1, and Bax. Further, the expression of insulin, glucagon, and proliferating cell nuclear antigen (PCNA), and TdT-mediated dUTP-biotin nick-end labeling (TUNEL) staining were examined. Results. In embryonic and fetal pancreatic tissues, Bcl-2 was not detected in any type of pancreatic cell (acinar, ductal, or islet). Bcl-XL was expressed in all types of pancreatic cells throughout the gestation. Mcl-1 was expressed in all types of pancreatic components, and strongly expressed in the margin of the islets. Bax, a pro-apoptotic protein, was expressed in all components. PCNA was strongly expressed in the embryonic and fetal pancreas, especially in early stages of gestation; however, TUNEL staining was negative in all samples. At least one antiapoptotic protein was expressed in all types of pancreatic cells. Conclusion. The results of the current study indicate that active proliferation and avoidance of apoptosis take place in embryonic and fetal pancreatic tissues, which may be controlled by particular combinations of apoptosis-related proteins. Among these proteins, Bcl-XL and Mcl-1 may play an important role in the proliferation and differentiation of the embryonic and fetal pancreas.

Original languageEnglish
Pages (from-to)113-122
Number of pages10
JournalInternational Journal of Pancreatology
Volume27
Issue number2
Publication statusPublished - 26-06-2000

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Fetus
Apoptosis
Pancreas
Proteins
Proliferating Cell Nuclear Antigen
Biotin
Pregnancy
Cell Growth Processes
bcl-X Protein
Staining and Labeling
Negative Staining
Apoptosis Regulatory Proteins
Acinar Cells
Growth
Fetal Development
Glucagon
Pancreatic Neoplasms
Islets of Langerhans
Neoplasms
Cell Cycle

All Science Journal Classification (ASJC) codes

  • Oncology
  • Endocrinology
  • Gastroenterology

Cite this

Kobayashi, H., Doi, R., Hosotani, R., Miyamoto, Y., Koshiba, T., Fujimoto, K., ... Imamura, M. (2000). Immunohistochemical analysis of apoptosis-related proteins in human embryonic and fetal pancreatic tissues. International Journal of Pancreatology, 27(2), 113-122.
Kobayashi, Hiroyuki ; Doi, Ryuichiro ; Hosotani, Ryo ; Miyamoto, Yoshiharu ; Koshiba, Takatomo ; Fujimoto, Koji ; Ida, Jun ; Tsuji, Shoichiro ; Nakajima, Sanae ; Kawaguchi, Michiya ; Shiota, Kohei ; Imamura, Masayuki. / Immunohistochemical analysis of apoptosis-related proteins in human embryonic and fetal pancreatic tissues. In: International Journal of Pancreatology. 2000 ; Vol. 27, No. 2. pp. 113-122.
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abstract = "Background. The growth of both cancer cells and fetal tissue is rapid; however, cancer cells de-differentiate and proliferate in a disorderly manner, whereas fetal tissues differentiate and proliferate in an orderly manner. Thus, there may be both common and different factors that are involved in the process of the uncontrolled cell growth of pancreatic cancers and the development of the fetal pancreas. The common part of the mechanisms should be in the regulation of the cell cycle, resulting in rapid proliferation via such mechanisms as growth stimulation and avoidance of apoptosis. Therefore, in the current study we investigated the expression of apoptosis-related proteins in fetal pancreatic tissues. Methods. Sixteen human embryonic and fetal pancreatic tissues obtained between 6 and 32 wk of gestation were used. We immunohistochemically examined the protein expression of Bcl-2, Bcl-XL, Mcl-1, and Bax. Further, the expression of insulin, glucagon, and proliferating cell nuclear antigen (PCNA), and TdT-mediated dUTP-biotin nick-end labeling (TUNEL) staining were examined. Results. In embryonic and fetal pancreatic tissues, Bcl-2 was not detected in any type of pancreatic cell (acinar, ductal, or islet). Bcl-XL was expressed in all types of pancreatic cells throughout the gestation. Mcl-1 was expressed in all types of pancreatic components, and strongly expressed in the margin of the islets. Bax, a pro-apoptotic protein, was expressed in all components. PCNA was strongly expressed in the embryonic and fetal pancreas, especially in early stages of gestation; however, TUNEL staining was negative in all samples. At least one antiapoptotic protein was expressed in all types of pancreatic cells. Conclusion. The results of the current study indicate that active proliferation and avoidance of apoptosis take place in embryonic and fetal pancreatic tissues, which may be controlled by particular combinations of apoptosis-related proteins. Among these proteins, Bcl-XL and Mcl-1 may play an important role in the proliferation and differentiation of the embryonic and fetal pancreas.",
author = "Hiroyuki Kobayashi and Ryuichiro Doi and Ryo Hosotani and Yoshiharu Miyamoto and Takatomo Koshiba and Koji Fujimoto and Jun Ida and Shoichiro Tsuji and Sanae Nakajima and Michiya Kawaguchi and Kohei Shiota and Masayuki Imamura",
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Kobayashi, H, Doi, R, Hosotani, R, Miyamoto, Y, Koshiba, T, Fujimoto, K, Ida, J, Tsuji, S, Nakajima, S, Kawaguchi, M, Shiota, K & Imamura, M 2000, 'Immunohistochemical analysis of apoptosis-related proteins in human embryonic and fetal pancreatic tissues', International Journal of Pancreatology, vol. 27, no. 2, pp. 113-122.

Immunohistochemical analysis of apoptosis-related proteins in human embryonic and fetal pancreatic tissues. / Kobayashi, Hiroyuki; Doi, Ryuichiro; Hosotani, Ryo; Miyamoto, Yoshiharu; Koshiba, Takatomo; Fujimoto, Koji; Ida, Jun; Tsuji, Shoichiro; Nakajima, Sanae; Kawaguchi, Michiya; Shiota, Kohei; Imamura, Masayuki.

In: International Journal of Pancreatology, Vol. 27, No. 2, 26.06.2000, p. 113-122.

Research output: Contribution to journalArticle

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T1 - Immunohistochemical analysis of apoptosis-related proteins in human embryonic and fetal pancreatic tissues

AU - Kobayashi, Hiroyuki

AU - Doi, Ryuichiro

AU - Hosotani, Ryo

AU - Miyamoto, Yoshiharu

AU - Koshiba, Takatomo

AU - Fujimoto, Koji

AU - Ida, Jun

AU - Tsuji, Shoichiro

AU - Nakajima, Sanae

AU - Kawaguchi, Michiya

AU - Shiota, Kohei

AU - Imamura, Masayuki

PY - 2000/6/26

Y1 - 2000/6/26

N2 - Background. The growth of both cancer cells and fetal tissue is rapid; however, cancer cells de-differentiate and proliferate in a disorderly manner, whereas fetal tissues differentiate and proliferate in an orderly manner. Thus, there may be both common and different factors that are involved in the process of the uncontrolled cell growth of pancreatic cancers and the development of the fetal pancreas. The common part of the mechanisms should be in the regulation of the cell cycle, resulting in rapid proliferation via such mechanisms as growth stimulation and avoidance of apoptosis. Therefore, in the current study we investigated the expression of apoptosis-related proteins in fetal pancreatic tissues. Methods. Sixteen human embryonic and fetal pancreatic tissues obtained between 6 and 32 wk of gestation were used. We immunohistochemically examined the protein expression of Bcl-2, Bcl-XL, Mcl-1, and Bax. Further, the expression of insulin, glucagon, and proliferating cell nuclear antigen (PCNA), and TdT-mediated dUTP-biotin nick-end labeling (TUNEL) staining were examined. Results. In embryonic and fetal pancreatic tissues, Bcl-2 was not detected in any type of pancreatic cell (acinar, ductal, or islet). Bcl-XL was expressed in all types of pancreatic cells throughout the gestation. Mcl-1 was expressed in all types of pancreatic components, and strongly expressed in the margin of the islets. Bax, a pro-apoptotic protein, was expressed in all components. PCNA was strongly expressed in the embryonic and fetal pancreas, especially in early stages of gestation; however, TUNEL staining was negative in all samples. At least one antiapoptotic protein was expressed in all types of pancreatic cells. Conclusion. The results of the current study indicate that active proliferation and avoidance of apoptosis take place in embryonic and fetal pancreatic tissues, which may be controlled by particular combinations of apoptosis-related proteins. Among these proteins, Bcl-XL and Mcl-1 may play an important role in the proliferation and differentiation of the embryonic and fetal pancreas.

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Kobayashi H, Doi R, Hosotani R, Miyamoto Y, Koshiba T, Fujimoto K et al. Immunohistochemical analysis of apoptosis-related proteins in human embryonic and fetal pancreatic tissues. International Journal of Pancreatology. 2000 Jun 26;27(2):113-122.