Immunohistochemical analysis of Bcl-2, Bax, Bcl-X, and Mcl-1 expression in pancreatic cancers

Yoshiharu Miyamoto, Ryo Hosotani, Michihiko Wada, Jeon Uk Lee, Takatomo Koshiba, Koji Fujimoto, Shoichiro Tsuji, Sanae Nakajima, Ryuichiro Doi, Masayuki Kato, Yuta Shimada, Masayuki Imamura

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

Expression of several members of the Bcl-2 family proteins was investigated by means of both immunohistochemical analysis in 30 invasive ductal adenocarcinomas and 23 intraductal papillary-mucinous tumors (IPMTs) and immunoblot analysis in 6 cancer tissues and 7 pancreatic cancer cell lines. We found that Bcl-2 was expressed in 23%, Bax in 53%, Bcl-X in 90%, and Mcl-1 in 90% of the invasive ductal adenocarcinomas. In intraductal papillary-mucinous adenocarcinomas, the expression rate of Bax was 44% and those of Bcl-XL and Mcl-1 were 88%; these values were higher than those for intraductal papillary-mucinous adenomas. Immunoblot analysis indentified Bcl-XL as the predominant form of the Bcl-X protein in both pancreatic cancer tissues and cell lines, and demonstrated that both Bcl-XL and Mcl-1 protein levels were uniformly high in all cell lines. These results suggest that an imbalance between antiapoptosis proteins (such as Bcl-2, Bcl-XL, and Mcl-1) and proapoptotic proteins (such as Bax and Bcl-Xs) is involved in the distinctive biologic features of adenocarcinomas of the pancreas. Furthermore, predominantly high expressions of Bcl-XL and Mcl-1 in intraductal papillary-mucinous adenocarcinomas might be involved in the carcinogenesis in IPMT of the pancreas.

Original languageEnglish
Pages (from-to)73-82
Number of pages10
JournalOncology
Volume56
Issue number1
DOIs
Publication statusPublished - 01-01-1999

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Pancreatic Neoplasms
Papillary Adenocarcinoma
Mucinous Adenocarcinoma
Adenocarcinoma
Cell Line
Pancreas
Proteins
bcl-X Protein
Neoplasms
Adenoma
Carcinogenesis

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Miyamoto, Y., Hosotani, R., Wada, M., Lee, J. U., Koshiba, T., Fujimoto, K., ... Imamura, M. (1999). Immunohistochemical analysis of Bcl-2, Bax, Bcl-X, and Mcl-1 expression in pancreatic cancers. Oncology, 56(1), 73-82. https://doi.org/10.1159/000011933
Miyamoto, Yoshiharu ; Hosotani, Ryo ; Wada, Michihiko ; Lee, Jeon Uk ; Koshiba, Takatomo ; Fujimoto, Koji ; Tsuji, Shoichiro ; Nakajima, Sanae ; Doi, Ryuichiro ; Kato, Masayuki ; Shimada, Yuta ; Imamura, Masayuki. / Immunohistochemical analysis of Bcl-2, Bax, Bcl-X, and Mcl-1 expression in pancreatic cancers. In: Oncology. 1999 ; Vol. 56, No. 1. pp. 73-82.
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abstract = "Expression of several members of the Bcl-2 family proteins was investigated by means of both immunohistochemical analysis in 30 invasive ductal adenocarcinomas and 23 intraductal papillary-mucinous tumors (IPMTs) and immunoblot analysis in 6 cancer tissues and 7 pancreatic cancer cell lines. We found that Bcl-2 was expressed in 23{\%}, Bax in 53{\%}, Bcl-X in 90{\%}, and Mcl-1 in 90{\%} of the invasive ductal adenocarcinomas. In intraductal papillary-mucinous adenocarcinomas, the expression rate of Bax was 44{\%} and those of Bcl-XL and Mcl-1 were 88{\%}; these values were higher than those for intraductal papillary-mucinous adenomas. Immunoblot analysis indentified Bcl-XL as the predominant form of the Bcl-X protein in both pancreatic cancer tissues and cell lines, and demonstrated that both Bcl-XL and Mcl-1 protein levels were uniformly high in all cell lines. These results suggest that an imbalance between antiapoptosis proteins (such as Bcl-2, Bcl-XL, and Mcl-1) and proapoptotic proteins (such as Bax and Bcl-Xs) is involved in the distinctive biologic features of adenocarcinomas of the pancreas. Furthermore, predominantly high expressions of Bcl-XL and Mcl-1 in intraductal papillary-mucinous adenocarcinomas might be involved in the carcinogenesis in IPMT of the pancreas.",
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Miyamoto, Y, Hosotani, R, Wada, M, Lee, JU, Koshiba, T, Fujimoto, K, Tsuji, S, Nakajima, S, Doi, R, Kato, M, Shimada, Y & Imamura, M 1999, 'Immunohistochemical analysis of Bcl-2, Bax, Bcl-X, and Mcl-1 expression in pancreatic cancers', Oncology, vol. 56, no. 1, pp. 73-82. https://doi.org/10.1159/000011933

Immunohistochemical analysis of Bcl-2, Bax, Bcl-X, and Mcl-1 expression in pancreatic cancers. / Miyamoto, Yoshiharu; Hosotani, Ryo; Wada, Michihiko; Lee, Jeon Uk; Koshiba, Takatomo; Fujimoto, Koji; Tsuji, Shoichiro; Nakajima, Sanae; Doi, Ryuichiro; Kato, Masayuki; Shimada, Yuta; Imamura, Masayuki.

In: Oncology, Vol. 56, No. 1, 01.01.1999, p. 73-82.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Immunohistochemical analysis of Bcl-2, Bax, Bcl-X, and Mcl-1 expression in pancreatic cancers

AU - Miyamoto, Yoshiharu

AU - Hosotani, Ryo

AU - Wada, Michihiko

AU - Lee, Jeon Uk

AU - Koshiba, Takatomo

AU - Fujimoto, Koji

AU - Tsuji, Shoichiro

AU - Nakajima, Sanae

AU - Doi, Ryuichiro

AU - Kato, Masayuki

AU - Shimada, Yuta

AU - Imamura, Masayuki

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Expression of several members of the Bcl-2 family proteins was investigated by means of both immunohistochemical analysis in 30 invasive ductal adenocarcinomas and 23 intraductal papillary-mucinous tumors (IPMTs) and immunoblot analysis in 6 cancer tissues and 7 pancreatic cancer cell lines. We found that Bcl-2 was expressed in 23%, Bax in 53%, Bcl-X in 90%, and Mcl-1 in 90% of the invasive ductal adenocarcinomas. In intraductal papillary-mucinous adenocarcinomas, the expression rate of Bax was 44% and those of Bcl-XL and Mcl-1 were 88%; these values were higher than those for intraductal papillary-mucinous adenomas. Immunoblot analysis indentified Bcl-XL as the predominant form of the Bcl-X protein in both pancreatic cancer tissues and cell lines, and demonstrated that both Bcl-XL and Mcl-1 protein levels were uniformly high in all cell lines. These results suggest that an imbalance between antiapoptosis proteins (such as Bcl-2, Bcl-XL, and Mcl-1) and proapoptotic proteins (such as Bax and Bcl-Xs) is involved in the distinctive biologic features of adenocarcinomas of the pancreas. Furthermore, predominantly high expressions of Bcl-XL and Mcl-1 in intraductal papillary-mucinous adenocarcinomas might be involved in the carcinogenesis in IPMT of the pancreas.

AB - Expression of several members of the Bcl-2 family proteins was investigated by means of both immunohistochemical analysis in 30 invasive ductal adenocarcinomas and 23 intraductal papillary-mucinous tumors (IPMTs) and immunoblot analysis in 6 cancer tissues and 7 pancreatic cancer cell lines. We found that Bcl-2 was expressed in 23%, Bax in 53%, Bcl-X in 90%, and Mcl-1 in 90% of the invasive ductal adenocarcinomas. In intraductal papillary-mucinous adenocarcinomas, the expression rate of Bax was 44% and those of Bcl-XL and Mcl-1 were 88%; these values were higher than those for intraductal papillary-mucinous adenomas. Immunoblot analysis indentified Bcl-XL as the predominant form of the Bcl-X protein in both pancreatic cancer tissues and cell lines, and demonstrated that both Bcl-XL and Mcl-1 protein levels were uniformly high in all cell lines. These results suggest that an imbalance between antiapoptosis proteins (such as Bcl-2, Bcl-XL, and Mcl-1) and proapoptotic proteins (such as Bax and Bcl-Xs) is involved in the distinctive biologic features of adenocarcinomas of the pancreas. Furthermore, predominantly high expressions of Bcl-XL and Mcl-1 in intraductal papillary-mucinous adenocarcinomas might be involved in the carcinogenesis in IPMT of the pancreas.

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Miyamoto Y, Hosotani R, Wada M, Lee JU, Koshiba T, Fujimoto K et al. Immunohistochemical analysis of Bcl-2, Bax, Bcl-X, and Mcl-1 expression in pancreatic cancers. Oncology. 1999 Jan 1;56(1):73-82. https://doi.org/10.1159/000011933