Immunohistochemical analysis of cyclooxygenase-2 expression in pancreatic tumors

Takatomo Koshiba, Ryo Hosotani, Yoshiharu Miyamoto, Michihiko Wada, Jeon Uk Lee, Koji Fujimoto, Shoichiro Tsuji, Sanae Nakajima, Ryuichiro Doi, Masayuki Imamura

Research output: Contribution to journalArticle

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Abstract

Background. A considerable amount of evidence collected from several experimental systems and clinical studies with nonsteroidal Anti-inflammatory drugs (NSAIDs) indicates that Cox-2 may play a major role in colorectal tumorigenesis, but little information about Cox-2 expression in pancreatic tumors is available. In this study, we investigated Cox-2 expression by means of both immunohistochemical analysis and immunoblot analysis in pancreatic tumors. Methods. Fifty invasive ductal adenocarcinomas and 26 intraductal papillary-mucinous tumors (IPMTs) were used for immunohistochemical analysis, and five pancreatic cancer tissues and five pancreatic cancer cell lines for immunoblot analysis. Results. Cox-2 was expressed in 72% of the invasive ductal adenocarcinomas, 31% of intraductal papillary-mucinous adenocarcinomas, and none of intraductal papillary-mucinous adenomas. The expression rate of Cox-2 in intraductal papillary-mucinous adenocarcinomas was significantly higher than that in intraductal papillary-mucinous adenomas, and that in invasive ductal adenocarcinomas was significantly higher than that in intraductal papillary-mucinous carcinomas. However, there was no significant correlation between Cox-2 expression and the prognosis and clinicopathological factors. Immunoblot analysis identified Cox-2 in all of pancreatic cancer tissues and 60% of cell lines. Conclusion. The biological role of cyclooxygenase-2 (Cox-2) in carcinoma cells should be investigated with reference to the cancer progression of the pancreas.

Original languageEnglish
Pages (from-to)69-76
Number of pages8
JournalInternational Journal of Pancreatology
Volume26
Issue number2
Publication statusPublished - 09-12-1999

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Cyclooxygenase 2
Pancreatic Neoplasms
Mucinous Adenocarcinoma
Neoplasms
Papillary Adenocarcinoma
Adenocarcinoma
Adenoma
Cell Line
Carcinoma, Intraductal, Noninfiltrating
Papillary Carcinoma
Carcinogenesis
Anti-Inflammatory Agents
Carcinoma
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Oncology
  • Endocrinology
  • Gastroenterology

Cite this

Koshiba, T., Hosotani, R., Miyamoto, Y., Wada, M., Lee, J. U., Fujimoto, K., ... Imamura, M. (1999). Immunohistochemical analysis of cyclooxygenase-2 expression in pancreatic tumors. International Journal of Pancreatology, 26(2), 69-76.
Koshiba, Takatomo ; Hosotani, Ryo ; Miyamoto, Yoshiharu ; Wada, Michihiko ; Lee, Jeon Uk ; Fujimoto, Koji ; Tsuji, Shoichiro ; Nakajima, Sanae ; Doi, Ryuichiro ; Imamura, Masayuki. / Immunohistochemical analysis of cyclooxygenase-2 expression in pancreatic tumors. In: International Journal of Pancreatology. 1999 ; Vol. 26, No. 2. pp. 69-76.
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abstract = "Background. A considerable amount of evidence collected from several experimental systems and clinical studies with nonsteroidal Anti-inflammatory drugs (NSAIDs) indicates that Cox-2 may play a major role in colorectal tumorigenesis, but little information about Cox-2 expression in pancreatic tumors is available. In this study, we investigated Cox-2 expression by means of both immunohistochemical analysis and immunoblot analysis in pancreatic tumors. Methods. Fifty invasive ductal adenocarcinomas and 26 intraductal papillary-mucinous tumors (IPMTs) were used for immunohistochemical analysis, and five pancreatic cancer tissues and five pancreatic cancer cell lines for immunoblot analysis. Results. Cox-2 was expressed in 72{\%} of the invasive ductal adenocarcinomas, 31{\%} of intraductal papillary-mucinous adenocarcinomas, and none of intraductal papillary-mucinous adenomas. The expression rate of Cox-2 in intraductal papillary-mucinous adenocarcinomas was significantly higher than that in intraductal papillary-mucinous adenomas, and that in invasive ductal adenocarcinomas was significantly higher than that in intraductal papillary-mucinous carcinomas. However, there was no significant correlation between Cox-2 expression and the prognosis and clinicopathological factors. Immunoblot analysis identified Cox-2 in all of pancreatic cancer tissues and 60{\%} of cell lines. Conclusion. The biological role of cyclooxygenase-2 (Cox-2) in carcinoma cells should be investigated with reference to the cancer progression of the pancreas.",
author = "Takatomo Koshiba and Ryo Hosotani and Yoshiharu Miyamoto and Michihiko Wada and Lee, {Jeon Uk} and Koji Fujimoto and Shoichiro Tsuji and Sanae Nakajima and Ryuichiro Doi and Masayuki Imamura",
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Koshiba, T, Hosotani, R, Miyamoto, Y, Wada, M, Lee, JU, Fujimoto, K, Tsuji, S, Nakajima, S, Doi, R & Imamura, M 1999, 'Immunohistochemical analysis of cyclooxygenase-2 expression in pancreatic tumors', International Journal of Pancreatology, vol. 26, no. 2, pp. 69-76.

Immunohistochemical analysis of cyclooxygenase-2 expression in pancreatic tumors. / Koshiba, Takatomo; Hosotani, Ryo; Miyamoto, Yoshiharu; Wada, Michihiko; Lee, Jeon Uk; Fujimoto, Koji; Tsuji, Shoichiro; Nakajima, Sanae; Doi, Ryuichiro; Imamura, Masayuki.

In: International Journal of Pancreatology, Vol. 26, No. 2, 09.12.1999, p. 69-76.

Research output: Contribution to journalArticle

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T1 - Immunohistochemical analysis of cyclooxygenase-2 expression in pancreatic tumors

AU - Koshiba, Takatomo

AU - Hosotani, Ryo

AU - Miyamoto, Yoshiharu

AU - Wada, Michihiko

AU - Lee, Jeon Uk

AU - Fujimoto, Koji

AU - Tsuji, Shoichiro

AU - Nakajima, Sanae

AU - Doi, Ryuichiro

AU - Imamura, Masayuki

PY - 1999/12/9

Y1 - 1999/12/9

N2 - Background. A considerable amount of evidence collected from several experimental systems and clinical studies with nonsteroidal Anti-inflammatory drugs (NSAIDs) indicates that Cox-2 may play a major role in colorectal tumorigenesis, but little information about Cox-2 expression in pancreatic tumors is available. In this study, we investigated Cox-2 expression by means of both immunohistochemical analysis and immunoblot analysis in pancreatic tumors. Methods. Fifty invasive ductal adenocarcinomas and 26 intraductal papillary-mucinous tumors (IPMTs) were used for immunohistochemical analysis, and five pancreatic cancer tissues and five pancreatic cancer cell lines for immunoblot analysis. Results. Cox-2 was expressed in 72% of the invasive ductal adenocarcinomas, 31% of intraductal papillary-mucinous adenocarcinomas, and none of intraductal papillary-mucinous adenomas. The expression rate of Cox-2 in intraductal papillary-mucinous adenocarcinomas was significantly higher than that in intraductal papillary-mucinous adenomas, and that in invasive ductal adenocarcinomas was significantly higher than that in intraductal papillary-mucinous carcinomas. However, there was no significant correlation between Cox-2 expression and the prognosis and clinicopathological factors. Immunoblot analysis identified Cox-2 in all of pancreatic cancer tissues and 60% of cell lines. Conclusion. The biological role of cyclooxygenase-2 (Cox-2) in carcinoma cells should be investigated with reference to the cancer progression of the pancreas.

AB - Background. A considerable amount of evidence collected from several experimental systems and clinical studies with nonsteroidal Anti-inflammatory drugs (NSAIDs) indicates that Cox-2 may play a major role in colorectal tumorigenesis, but little information about Cox-2 expression in pancreatic tumors is available. In this study, we investigated Cox-2 expression by means of both immunohistochemical analysis and immunoblot analysis in pancreatic tumors. Methods. Fifty invasive ductal adenocarcinomas and 26 intraductal papillary-mucinous tumors (IPMTs) were used for immunohistochemical analysis, and five pancreatic cancer tissues and five pancreatic cancer cell lines for immunoblot analysis. Results. Cox-2 was expressed in 72% of the invasive ductal adenocarcinomas, 31% of intraductal papillary-mucinous adenocarcinomas, and none of intraductal papillary-mucinous adenomas. The expression rate of Cox-2 in intraductal papillary-mucinous adenocarcinomas was significantly higher than that in intraductal papillary-mucinous adenomas, and that in invasive ductal adenocarcinomas was significantly higher than that in intraductal papillary-mucinous carcinomas. However, there was no significant correlation between Cox-2 expression and the prognosis and clinicopathological factors. Immunoblot analysis identified Cox-2 in all of pancreatic cancer tissues and 60% of cell lines. Conclusion. The biological role of cyclooxygenase-2 (Cox-2) in carcinoma cells should be investigated with reference to the cancer progression of the pancreas.

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Koshiba T, Hosotani R, Miyamoto Y, Wada M, Lee JU, Fujimoto K et al. Immunohistochemical analysis of cyclooxygenase-2 expression in pancreatic tumors. International Journal of Pancreatology. 1999 Dec 9;26(2):69-76.