Immunohistochemical analysis of various serum proteins in living mouse thymus

Yuqin Bai, Bao Wu, Nobuo Terada, Yurika Saitoh, Nobuhiko Ohno, Sei Saitoh, Shinichi Ohno

Research output: Chapter in Book/Report/Conference proceedingChapter


It has been difficult to clarify the precise localizations of soluble serum proteins in thymic tissues of living animals with conventional immersion or perfusion fixation followed by alcohol dehydration owing to ischemia and anoxia. In this study, “in vivo cryotechnique” (IVCT) followed by freeze-substitution fixation was performed to examine the thymic structures of living mice and immunolocalizations of intrinsic or extrinsic serum proteins, which were albumin, immunoglobulin G1 (IgG1), IgA, and IgM, as well as intravenously injected bovine serum albumin (BSA). Mouse albumin was more clearly immunolocalized in blood vessels and interstitial matrices of the thymic cortex than in tissues prepared by the conventional methods. The immunoreactivities of albumin and IgG1 were stronger than those of IgA and IgM in the interstitium of subcapsular cortex. The injected BSA was timedependently immunolocalized in blood vessels and the interstitium of corticomedullary areas at 3.5 h after its injection and then gradually diffused into the interstitium of the whole cortex at 6 and 12 h. Thus, IVCT revealed defi nite immunolocalizations of serum albumin and IgG1 in the interstitium of thymus of living mice, indicating different accessibility of serum proteins from the corticomedullary areas, not from the subcapsular cortex of living animals, depending on various molecular sizes and concentrations.

Original languageEnglish
Title of host publicationIn Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs
PublisherSpringer Japan
Number of pages6
ISBN (Electronic)9784431557234
ISBN (Print)9784431557227
Publication statusPublished - 01-01-2015
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General Medicine
  • General Biochemistry,Genetics and Molecular Biology
  • General Agricultural and Biological Sciences


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