TY - JOUR
T1 - Immunohistochemical detection of meconium in the fetal membrane, placenta and umbilical cord
AU - Furuta, N.
AU - Yaguchi, C.
AU - Itoh, H.
AU - Morishima, Y.
AU - Tamura, N.
AU - Kato, M.
AU - Uchida, T.
AU - Suzuki, K.
AU - Sugihara, K.
AU - Kawabata, Y.
AU - Suzuki, N.
AU - Sasaki, T.
AU - Horiuchi, K.
AU - Kanayama, N.
N1 - Funding Information:
This work was supported in part by a Grant-in-aid for Scientific Research from the Ministry of Education, Science, Culture and Sports , Japan (No. 21390322 and No. 23659534 ) and grants from Kanzawa Medical Research Foundation , Society for Women's Health Science Research , and The Food Science Institute Foundation .
PY - 2012/1
Y1 - 2012/1
N2 - Objective: To develop the immunohistochemistry specific for meconium in the placenta, fetal membrane and umbilical cord. Study design: We previously reported the specific presence of zinc coproporphyrin I (ZnCP-I) in human meconium and demonstrated the possible diagnostic use of an elevation in maternal plasma ZnCP-I levels in cases of amniotic fluid embolism. In this study, we developed a new specific monoclonal antibody for ZnCP-I and applied it to the immunostaining of meconium in the placenta, fetal membrane, and umbilical cord. Results: Immunoreactivity of ZnCP-I clearly and specifically identified meconium in the placenta, fetal membrane, and umbilical cord. It was especially useful in cases of severe chorioamnionitis to detect meconium in the macrophages surrounded by numerous neutrophils. In more than half of the cases, meconium was detected in clear amniotic fluid at delivery, suggesting previous exposure. Conclusions: Immunohistochemical detection of ZnCP-I is a highly sensitive histological diagnosis of meconium.
AB - Objective: To develop the immunohistochemistry specific for meconium in the placenta, fetal membrane and umbilical cord. Study design: We previously reported the specific presence of zinc coproporphyrin I (ZnCP-I) in human meconium and demonstrated the possible diagnostic use of an elevation in maternal plasma ZnCP-I levels in cases of amniotic fluid embolism. In this study, we developed a new specific monoclonal antibody for ZnCP-I and applied it to the immunostaining of meconium in the placenta, fetal membrane, and umbilical cord. Results: Immunoreactivity of ZnCP-I clearly and specifically identified meconium in the placenta, fetal membrane, and umbilical cord. It was especially useful in cases of severe chorioamnionitis to detect meconium in the macrophages surrounded by numerous neutrophils. In more than half of the cases, meconium was detected in clear amniotic fluid at delivery, suggesting previous exposure. Conclusions: Immunohistochemical detection of ZnCP-I is a highly sensitive histological diagnosis of meconium.
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U2 - 10.1016/j.placenta.2011.10.007
DO - 10.1016/j.placenta.2011.10.007
M3 - Article
C2 - 22041294
AN - SCOPUS:84655174998
VL - 33
SP - 24
EP - 30
JO - Placenta
JF - Placenta
SN - 0143-4004
IS - 1
ER -