Immunohistochemical detection of meconium in the fetal membrane, placenta and umbilical cord

N. Furuta, C. Yaguchi, H. Itoh, Y. Morishima, N. Tamura, M. Kato, T. Uchida, K. Suzuki, K. Sugihara, Y. Kawabata, N. Suzuki, T. Sasaki, K. Horiuchi, N. Kanayama

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objective: To develop the immunohistochemistry specific for meconium in the placenta, fetal membrane and umbilical cord. Study design: We previously reported the specific presence of zinc coproporphyrin I (ZnCP-I) in human meconium and demonstrated the possible diagnostic use of an elevation in maternal plasma ZnCP-I levels in cases of amniotic fluid embolism. In this study, we developed a new specific monoclonal antibody for ZnCP-I and applied it to the immunostaining of meconium in the placenta, fetal membrane, and umbilical cord. Results: Immunoreactivity of ZnCP-I clearly and specifically identified meconium in the placenta, fetal membrane, and umbilical cord. It was especially useful in cases of severe chorioamnionitis to detect meconium in the macrophages surrounded by numerous neutrophils. In more than half of the cases, meconium was detected in clear amniotic fluid at delivery, suggesting previous exposure. Conclusions: Immunohistochemical detection of ZnCP-I is a highly sensitive histological diagnosis of meconium.

Original languageEnglish
Pages (from-to)24-30
Number of pages7
JournalPlacenta
Volume33
Issue number1
DOIs
Publication statusPublished - 01-2012

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynaecology
  • Developmental Biology

Fingerprint Dive into the research topics of 'Immunohistochemical detection of meconium in the fetal membrane, placenta and umbilical cord'. Together they form a unique fingerprint.

  • Cite this

    Furuta, N., Yaguchi, C., Itoh, H., Morishima, Y., Tamura, N., Kato, M., Uchida, T., Suzuki, K., Sugihara, K., Kawabata, Y., Suzuki, N., Sasaki, T., Horiuchi, K., & Kanayama, N. (2012). Immunohistochemical detection of meconium in the fetal membrane, placenta and umbilical cord. Placenta, 33(1), 24-30. https://doi.org/10.1016/j.placenta.2011.10.007