Abstract
Hepatocyte growth factor/scatter factor (HGF/SF), which has various physiological functions, and its receptor c-Met, the human c-met proto-oncogene product, are thought to be determinant in the pathological processes of various malignancies. To investigate the possible role of HGF/SF in the progression of development of astrocytic tumors, we examined the expression of c-Met in these tumors. Immunohistochemistry using the streptavidin-biotin peroxidase complex method and immunofluorescence double staining with anti-c-Met polyclonal and anti-glial fibrillary acidic protein monoclonal antibodies were performed. Positive c-Met expression was detected in 31 of the 42 astrocytic tumors and some of the control cases analyzed. c-Met-positive cells showed morphological characteristics of astrocytes. Especially in the cases of high-grade tumors, c-Met positivity was abundant in cells in both vascular-rich and peripheral regions of the tumors but not in the cells with distinctly malignant features. Immunofluorescence double staining revealed that the c-Met-positive cells were in part of astrocytic origin. We suggest that c-Met-positive cells are affected by some factors in the lesions where the pathological processes are in a state of development. Our studies indicated that c-Met expression might take part in glioma invasion but not in the development of malignancy.
| Original language | English |
|---|---|
| Pages (from-to) | 345-351 |
| Number of pages | 7 |
| Journal | Acta Neuropathologica |
| Volume | 95 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 09-04-1998 |
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All Science Journal Classification (ASJC) codes
- Pathology and Forensic Medicine
- Clinical Neurology
- Cellular and Molecular Neuroscience
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Immunohistochemical examination of c-Met protein expression in astrocytic tumors. / Hirose, Yuichi; Kojima, Masaru; Sagoh, Masachika; Murakami, Hideki; Yoshida, Kazunari; Shimazaki, Kenji; Kawase, Takeshi.
In: Acta Neuropathologica, Vol. 95, No. 4, 09.04.1998, p. 345-351.Research output: Contribution to journal › Article
TY - JOUR
T1 - Immunohistochemical examination of c-Met protein expression in astrocytic tumors
AU - Hirose, Yuichi
AU - Kojima, Masaru
AU - Sagoh, Masachika
AU - Murakami, Hideki
AU - Yoshida, Kazunari
AU - Shimazaki, Kenji
AU - Kawase, Takeshi
PY - 1998/4/9
Y1 - 1998/4/9
N2 - Hepatocyte growth factor/scatter factor (HGF/SF), which has various physiological functions, and its receptor c-Met, the human c-met proto-oncogene product, are thought to be determinant in the pathological processes of various malignancies. To investigate the possible role of HGF/SF in the progression of development of astrocytic tumors, we examined the expression of c-Met in these tumors. Immunohistochemistry using the streptavidin-biotin peroxidase complex method and immunofluorescence double staining with anti-c-Met polyclonal and anti-glial fibrillary acidic protein monoclonal antibodies were performed. Positive c-Met expression was detected in 31 of the 42 astrocytic tumors and some of the control cases analyzed. c-Met-positive cells showed morphological characteristics of astrocytes. Especially in the cases of high-grade tumors, c-Met positivity was abundant in cells in both vascular-rich and peripheral regions of the tumors but not in the cells with distinctly malignant features. Immunofluorescence double staining revealed that the c-Met-positive cells were in part of astrocytic origin. We suggest that c-Met-positive cells are affected by some factors in the lesions where the pathological processes are in a state of development. Our studies indicated that c-Met expression might take part in glioma invasion but not in the development of malignancy.
AB - Hepatocyte growth factor/scatter factor (HGF/SF), which has various physiological functions, and its receptor c-Met, the human c-met proto-oncogene product, are thought to be determinant in the pathological processes of various malignancies. To investigate the possible role of HGF/SF in the progression of development of astrocytic tumors, we examined the expression of c-Met in these tumors. Immunohistochemistry using the streptavidin-biotin peroxidase complex method and immunofluorescence double staining with anti-c-Met polyclonal and anti-glial fibrillary acidic protein monoclonal antibodies were performed. Positive c-Met expression was detected in 31 of the 42 astrocytic tumors and some of the control cases analyzed. c-Met-positive cells showed morphological characteristics of astrocytes. Especially in the cases of high-grade tumors, c-Met positivity was abundant in cells in both vascular-rich and peripheral regions of the tumors but not in the cells with distinctly malignant features. Immunofluorescence double staining revealed that the c-Met-positive cells were in part of astrocytic origin. We suggest that c-Met-positive cells are affected by some factors in the lesions where the pathological processes are in a state of development. Our studies indicated that c-Met expression might take part in glioma invasion but not in the development of malignancy.
UR - http://www.scopus.com/inward/record.url?scp=0031913354&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031913354&partnerID=8YFLogxK
U2 - 10.1007/s004010050809
DO - 10.1007/s004010050809
M3 - Article
C2 - 9560011
AN - SCOPUS:0031913354
VL - 95
SP - 345
EP - 351
JO - Acta Neuropathologica
JF - Acta Neuropathologica
SN - 0001-6322
IS - 4
ER -