Immunohistochemical examination of c-Met protein expression in astrocytic tumors

Yuichi Hirose, Masaru Kojima, Masachika Sagoh, Hideki Murakami, Kazunari Yoshida, Kenji Shimazaki, Takeshi Kawase

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Hepatocyte growth factor/scatter factor (HGF/SF), which has various physiological functions, and its receptor c-Met, the human c-met proto-oncogene product, are thought to be determinant in the pathological processes of various malignancies. To investigate the possible role of HGF/SF in the progression of development of astrocytic tumors, we examined the expression of c-Met in these tumors. Immunohistochemistry using the streptavidin-biotin peroxidase complex method and immunofluorescence double staining with anti-c-Met polyclonal and anti-glial fibrillary acidic protein monoclonal antibodies were performed. Positive c-Met expression was detected in 31 of the 42 astrocytic tumors and some of the control cases analyzed. c-Met-positive cells showed morphological characteristics of astrocytes. Especially in the cases of high-grade tumors, c-Met positivity was abundant in cells in both vascular-rich and peripheral regions of the tumors but not in the cells with distinctly malignant features. Immunofluorescence double staining revealed that the c-Met-positive cells were in part of astrocytic origin. We suggest that c-Met-positive cells are affected by some factors in the lesions where the pathological processes are in a state of development. Our studies indicated that c-Met expression might take part in glioma invasion but not in the development of malignancy.

Original languageEnglish
Pages (from-to)345-351
Number of pages7
JournalActa Neuropathologica
Volume95
Issue number4
DOIs
Publication statusPublished - 09-04-1998

Fingerprint

Proto-Oncogene Proteins c-met
Hepatocyte Growth Factor
Neoplasms
Pathologic Processes
Fluorescent Antibody Technique
Staining and Labeling
Proto-Oncogenes
Oncogene Proteins
Glial Fibrillary Acidic Protein
Glioma
Astrocytes
Peroxidase
Blood Vessels
Immunohistochemistry
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

Cite this

Hirose, Y., Kojima, M., Sagoh, M., Murakami, H., Yoshida, K., Shimazaki, K., & Kawase, T. (1998). Immunohistochemical examination of c-Met protein expression in astrocytic tumors. Acta Neuropathologica, 95(4), 345-351. https://doi.org/10.1007/s004010050809
Hirose, Yuichi ; Kojima, Masaru ; Sagoh, Masachika ; Murakami, Hideki ; Yoshida, Kazunari ; Shimazaki, Kenji ; Kawase, Takeshi. / Immunohistochemical examination of c-Met protein expression in astrocytic tumors. In: Acta Neuropathologica. 1998 ; Vol. 95, No. 4. pp. 345-351.
@article{5b294ddd0e644a3b9be612130e9ddcc7,
title = "Immunohistochemical examination of c-Met protein expression in astrocytic tumors",
abstract = "Hepatocyte growth factor/scatter factor (HGF/SF), which has various physiological functions, and its receptor c-Met, the human c-met proto-oncogene product, are thought to be determinant in the pathological processes of various malignancies. To investigate the possible role of HGF/SF in the progression of development of astrocytic tumors, we examined the expression of c-Met in these tumors. Immunohistochemistry using the streptavidin-biotin peroxidase complex method and immunofluorescence double staining with anti-c-Met polyclonal and anti-glial fibrillary acidic protein monoclonal antibodies were performed. Positive c-Met expression was detected in 31 of the 42 astrocytic tumors and some of the control cases analyzed. c-Met-positive cells showed morphological characteristics of astrocytes. Especially in the cases of high-grade tumors, c-Met positivity was abundant in cells in both vascular-rich and peripheral regions of the tumors but not in the cells with distinctly malignant features. Immunofluorescence double staining revealed that the c-Met-positive cells were in part of astrocytic origin. We suggest that c-Met-positive cells are affected by some factors in the lesions where the pathological processes are in a state of development. Our studies indicated that c-Met expression might take part in glioma invasion but not in the development of malignancy.",
author = "Yuichi Hirose and Masaru Kojima and Masachika Sagoh and Hideki Murakami and Kazunari Yoshida and Kenji Shimazaki and Takeshi Kawase",
year = "1998",
month = "4",
day = "9",
doi = "10.1007/s004010050809",
language = "English",
volume = "95",
pages = "345--351",
journal = "Acta Neuropathologica",
issn = "0001-6322",
publisher = "Springer Verlag",
number = "4",

}

Hirose, Y, Kojima, M, Sagoh, M, Murakami, H, Yoshida, K, Shimazaki, K & Kawase, T 1998, 'Immunohistochemical examination of c-Met protein expression in astrocytic tumors', Acta Neuropathologica, vol. 95, no. 4, pp. 345-351. https://doi.org/10.1007/s004010050809

Immunohistochemical examination of c-Met protein expression in astrocytic tumors. / Hirose, Yuichi; Kojima, Masaru; Sagoh, Masachika; Murakami, Hideki; Yoshida, Kazunari; Shimazaki, Kenji; Kawase, Takeshi.

In: Acta Neuropathologica, Vol. 95, No. 4, 09.04.1998, p. 345-351.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Immunohistochemical examination of c-Met protein expression in astrocytic tumors

AU - Hirose, Yuichi

AU - Kojima, Masaru

AU - Sagoh, Masachika

AU - Murakami, Hideki

AU - Yoshida, Kazunari

AU - Shimazaki, Kenji

AU - Kawase, Takeshi

PY - 1998/4/9

Y1 - 1998/4/9

N2 - Hepatocyte growth factor/scatter factor (HGF/SF), which has various physiological functions, and its receptor c-Met, the human c-met proto-oncogene product, are thought to be determinant in the pathological processes of various malignancies. To investigate the possible role of HGF/SF in the progression of development of astrocytic tumors, we examined the expression of c-Met in these tumors. Immunohistochemistry using the streptavidin-biotin peroxidase complex method and immunofluorescence double staining with anti-c-Met polyclonal and anti-glial fibrillary acidic protein monoclonal antibodies were performed. Positive c-Met expression was detected in 31 of the 42 astrocytic tumors and some of the control cases analyzed. c-Met-positive cells showed morphological characteristics of astrocytes. Especially in the cases of high-grade tumors, c-Met positivity was abundant in cells in both vascular-rich and peripheral regions of the tumors but not in the cells with distinctly malignant features. Immunofluorescence double staining revealed that the c-Met-positive cells were in part of astrocytic origin. We suggest that c-Met-positive cells are affected by some factors in the lesions where the pathological processes are in a state of development. Our studies indicated that c-Met expression might take part in glioma invasion but not in the development of malignancy.

AB - Hepatocyte growth factor/scatter factor (HGF/SF), which has various physiological functions, and its receptor c-Met, the human c-met proto-oncogene product, are thought to be determinant in the pathological processes of various malignancies. To investigate the possible role of HGF/SF in the progression of development of astrocytic tumors, we examined the expression of c-Met in these tumors. Immunohistochemistry using the streptavidin-biotin peroxidase complex method and immunofluorescence double staining with anti-c-Met polyclonal and anti-glial fibrillary acidic protein monoclonal antibodies were performed. Positive c-Met expression was detected in 31 of the 42 astrocytic tumors and some of the control cases analyzed. c-Met-positive cells showed morphological characteristics of astrocytes. Especially in the cases of high-grade tumors, c-Met positivity was abundant in cells in both vascular-rich and peripheral regions of the tumors but not in the cells with distinctly malignant features. Immunofluorescence double staining revealed that the c-Met-positive cells were in part of astrocytic origin. We suggest that c-Met-positive cells are affected by some factors in the lesions where the pathological processes are in a state of development. Our studies indicated that c-Met expression might take part in glioma invasion but not in the development of malignancy.

UR - http://www.scopus.com/inward/record.url?scp=0031913354&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031913354&partnerID=8YFLogxK

U2 - 10.1007/s004010050809

DO - 10.1007/s004010050809

M3 - Article

C2 - 9560011

AN - SCOPUS:0031913354

VL - 95

SP - 345

EP - 351

JO - Acta Neuropathologica

JF - Acta Neuropathologica

SN - 0001-6322

IS - 4

ER -