Immunohistochemical localization and mRNA expression of apolipoprotein A-I in rat spinal cord.

Hidehiko Fujii, Kuniaki Saito, Hidetsugu Hamakawa, Naoya Maekawa, Suwako Fujigaki, Hisayasu Wada, Kuniyasu Shimokawa, Mitsuru Seishima, Tsuyoshi Takami

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Abstract

Apolipoproteins in the cerebrospinal fluid (CSF) play important roles in lipid metabolism in the central nervous system. Although it has been demonstrated that apo E is synthesized in the neuron, the synthesis of apo A-I has only been determined in fish and chicken. It was demonstrated that apo A-I concentrations in the CSF were increased in poliovirus-infected macaques, however, the origin of the CSF apo A-I was not determined. The present immunohistochemical study provided evidence that apo A-I was localized within the nerve cell body of the rat spinal cord. In situ hybridization also showed that apo A-I mRNA was predominantly expressed in the neurons. As a further experiment, we compared apo A-I levels in the spinal cord from control rats and rats with experimental allergic encephalomyelitis (EAE), which was induced by sensitization with myelin basic protein. Although no significant changes in serum apo A-I levels were observed, apo A-I levels in the spinal cord were significantly elevated in EAE rats. Furthermore, apo A-I in the spinal cord of rats with EAE was not seen in the nerve cell body, but at the interstitium, particularly in lesions where inflammation had occurred. The current study clearly demonstrated that apo A-I is synthesized in the neurons of the rat spinal cord and the synthesis was suppressed in EAE rats.

Original languageEnglish
Pages (from-to)93-98
Number of pages6
JournalJournal of atherosclerosis and thrombosis
Volume9
Issue number2
DOIs
Publication statusPublished - 2002

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All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Cardiology and Cardiovascular Medicine
  • Biochemistry, medical

Cite this

Fujii, H., Saito, K., Hamakawa, H., Maekawa, N., Fujigaki, S., Wada, H., Shimokawa, K., Seishima, M., & Takami, T. (2002). Immunohistochemical localization and mRNA expression of apolipoprotein A-I in rat spinal cord. Journal of atherosclerosis and thrombosis, 9(2), 93-98. https://doi.org/10.5551/jat.9.93