TY - JOUR
T1 - Immunohistochemical localization of mitochondrial fatty acid β-oxidation enzymes in Müller cells of the retina
AU - Atsuzawa, Kimie
AU - Nakazawa, Ayami
AU - Mizutani, Kenmei
AU - Fukasawa, Motoaki
AU - Yamamoto, Naoki
AU - Hashimoto, Takashi
AU - Usuda, Nobuteru
N1 - Funding Information:
Acknowledgments The authors are grateful to Mr. Y. Kinoshita for his continuing secretarial assistance. This research was partially supported by a Grant-in-aid for young scientists (no. 20791286) for Kimie Atsuzawa and a matching fund subsidy for private universities for Nobuteru Usuda, from the Ministry of Education, Cultures, Sports, Science and Technology of Japan.
PY - 2010/12
Y1 - 2010/12
N2 - The presence of a mitochondrial fatty acid β-oxidation system in the retina was shown by immunohistochemistry. Fatty acids are considered to serve as a major energy source metabolized by fatty acid β-oxidation together with glucose metabolized by glycolysis in the organs of the entire body, but almost nothing is known about this metabolic system in the retina. Adult rat retinae were subjected to immunofluorescence and immuno-electron microscopy for the localization of fatty acid β-oxidation enzymes, together with western blot analysis for quantitation of the amount of enzyme proteins and DNA microarray analysis for gene expression. All the enzymes examined were shown to be present in the retina, but in small amounts, with the amount of protein and gene expression in the retina being about 1/10 of those in the liver. Immunohistochemistry at light and electron microscopic levels revealed the enzymes to be more preferentially localized to the mitochondria of Müller cells than the retinal neurons. The Müller cells were isolated from the retina and confirmed for the presence of mitochondrial fatty acid β-oxidation enzymes. A mitochondrial fatty acid β-oxidation system was thus shown to be present in the retina heterogeneously.
AB - The presence of a mitochondrial fatty acid β-oxidation system in the retina was shown by immunohistochemistry. Fatty acids are considered to serve as a major energy source metabolized by fatty acid β-oxidation together with glucose metabolized by glycolysis in the organs of the entire body, but almost nothing is known about this metabolic system in the retina. Adult rat retinae were subjected to immunofluorescence and immuno-electron microscopy for the localization of fatty acid β-oxidation enzymes, together with western blot analysis for quantitation of the amount of enzyme proteins and DNA microarray analysis for gene expression. All the enzymes examined were shown to be present in the retina, but in small amounts, with the amount of protein and gene expression in the retina being about 1/10 of those in the liver. Immunohistochemistry at light and electron microscopic levels revealed the enzymes to be more preferentially localized to the mitochondria of Müller cells than the retinal neurons. The Müller cells were isolated from the retina and confirmed for the presence of mitochondrial fatty acid β-oxidation enzymes. A mitochondrial fatty acid β-oxidation system was thus shown to be present in the retina heterogeneously.
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U2 - 10.1007/s00418-010-0752-4
DO - 10.1007/s00418-010-0752-4
M3 - Article
C2 - 21046137
AN - SCOPUS:78651352218
SN - 0948-6143
VL - 134
SP - 565
EP - 579
JO - Histochemistry and Cell Biology
JF - Histochemistry and Cell Biology
IS - 6
ER -