Immunohistochemical localization of mitochondrial fatty acid β-oxidation enzymes in Müller cells of the retina

Kimie Atsuzawa, Ayami Nakazawa, Kenmei Mizutani, Motoaki Fukasawa, Naoki Yamamoto, Takashi Hashimoto, Nobuteru Usuda

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The presence of a mitochondrial fatty acid β-oxidation system in the retina was shown by immunohistochemistry. Fatty acids are considered to serve as a major energy source metabolized by fatty acid β-oxidation together with glucose metabolized by glycolysis in the organs of the entire body, but almost nothing is known about this metabolic system in the retina. Adult rat retinae were subjected to immunofluorescence and immuno-electron microscopy for the localization of fatty acid β-oxidation enzymes, together with western blot analysis for quantitation of the amount of enzyme proteins and DNA microarray analysis for gene expression. All the enzymes examined were shown to be present in the retina, but in small amounts, with the amount of protein and gene expression in the retina being about 1/10 of those in the liver. Immunohistochemistry at light and electron microscopic levels revealed the enzymes to be more preferentially localized to the mitochondria of Müller cells than the retinal neurons. The Müller cells were isolated from the retina and confirmed for the presence of mitochondrial fatty acid β-oxidation enzymes. A mitochondrial fatty acid β-oxidation system was thus shown to be present in the retina heterogeneously.

Original languageEnglish
Pages (from-to)565-579
Number of pages15
JournalHistochemistry and Cell Biology
Volume134
Issue number6
DOIs
Publication statusPublished - 01-12-2010

Fingerprint

Retina
Fatty Acids
Enzymes
Immunohistochemistry
Retinal Neurons
Gene Expression
Protein Array Analysis
Immunoelectron Microscopy
Glycolysis
Oligonucleotide Array Sequence Analysis
Fluorescent Antibody Technique
Mitochondria
Western Blotting
Electrons
Light
Glucose
Liver
Proteins

All Science Journal Classification (ASJC) codes

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

Cite this

Atsuzawa, Kimie ; Nakazawa, Ayami ; Mizutani, Kenmei ; Fukasawa, Motoaki ; Yamamoto, Naoki ; Hashimoto, Takashi ; Usuda, Nobuteru. / Immunohistochemical localization of mitochondrial fatty acid β-oxidation enzymes in Müller cells of the retina. In: Histochemistry and Cell Biology. 2010 ; Vol. 134, No. 6. pp. 565-579.
@article{9578315f82d04dd485841391897d964a,
title = "Immunohistochemical localization of mitochondrial fatty acid β-oxidation enzymes in M{\"u}ller cells of the retina",
abstract = "The presence of a mitochondrial fatty acid β-oxidation system in the retina was shown by immunohistochemistry. Fatty acids are considered to serve as a major energy source metabolized by fatty acid β-oxidation together with glucose metabolized by glycolysis in the organs of the entire body, but almost nothing is known about this metabolic system in the retina. Adult rat retinae were subjected to immunofluorescence and immuno-electron microscopy for the localization of fatty acid β-oxidation enzymes, together with western blot analysis for quantitation of the amount of enzyme proteins and DNA microarray analysis for gene expression. All the enzymes examined were shown to be present in the retina, but in small amounts, with the amount of protein and gene expression in the retina being about 1/10 of those in the liver. Immunohistochemistry at light and electron microscopic levels revealed the enzymes to be more preferentially localized to the mitochondria of M{\"u}ller cells than the retinal neurons. The M{\"u}ller cells were isolated from the retina and confirmed for the presence of mitochondrial fatty acid β-oxidation enzymes. A mitochondrial fatty acid β-oxidation system was thus shown to be present in the retina heterogeneously.",
author = "Kimie Atsuzawa and Ayami Nakazawa and Kenmei Mizutani and Motoaki Fukasawa and Naoki Yamamoto and Takashi Hashimoto and Nobuteru Usuda",
year = "2010",
month = "12",
day = "1",
doi = "10.1007/s00418-010-0752-4",
language = "English",
volume = "134",
pages = "565--579",
journal = "Histochemistry and Cell Biology",
issn = "0948-6143",
publisher = "Springer Verlag",
number = "6",

}

Immunohistochemical localization of mitochondrial fatty acid β-oxidation enzymes in Müller cells of the retina. / Atsuzawa, Kimie; Nakazawa, Ayami; Mizutani, Kenmei; Fukasawa, Motoaki; Yamamoto, Naoki; Hashimoto, Takashi; Usuda, Nobuteru.

In: Histochemistry and Cell Biology, Vol. 134, No. 6, 01.12.2010, p. 565-579.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Immunohistochemical localization of mitochondrial fatty acid β-oxidation enzymes in Müller cells of the retina

AU - Atsuzawa, Kimie

AU - Nakazawa, Ayami

AU - Mizutani, Kenmei

AU - Fukasawa, Motoaki

AU - Yamamoto, Naoki

AU - Hashimoto, Takashi

AU - Usuda, Nobuteru

PY - 2010/12/1

Y1 - 2010/12/1

N2 - The presence of a mitochondrial fatty acid β-oxidation system in the retina was shown by immunohistochemistry. Fatty acids are considered to serve as a major energy source metabolized by fatty acid β-oxidation together with glucose metabolized by glycolysis in the organs of the entire body, but almost nothing is known about this metabolic system in the retina. Adult rat retinae were subjected to immunofluorescence and immuno-electron microscopy for the localization of fatty acid β-oxidation enzymes, together with western blot analysis for quantitation of the amount of enzyme proteins and DNA microarray analysis for gene expression. All the enzymes examined were shown to be present in the retina, but in small amounts, with the amount of protein and gene expression in the retina being about 1/10 of those in the liver. Immunohistochemistry at light and electron microscopic levels revealed the enzymes to be more preferentially localized to the mitochondria of Müller cells than the retinal neurons. The Müller cells were isolated from the retina and confirmed for the presence of mitochondrial fatty acid β-oxidation enzymes. A mitochondrial fatty acid β-oxidation system was thus shown to be present in the retina heterogeneously.

AB - The presence of a mitochondrial fatty acid β-oxidation system in the retina was shown by immunohistochemistry. Fatty acids are considered to serve as a major energy source metabolized by fatty acid β-oxidation together with glucose metabolized by glycolysis in the organs of the entire body, but almost nothing is known about this metabolic system in the retina. Adult rat retinae were subjected to immunofluorescence and immuno-electron microscopy for the localization of fatty acid β-oxidation enzymes, together with western blot analysis for quantitation of the amount of enzyme proteins and DNA microarray analysis for gene expression. All the enzymes examined were shown to be present in the retina, but in small amounts, with the amount of protein and gene expression in the retina being about 1/10 of those in the liver. Immunohistochemistry at light and electron microscopic levels revealed the enzymes to be more preferentially localized to the mitochondria of Müller cells than the retinal neurons. The Müller cells were isolated from the retina and confirmed for the presence of mitochondrial fatty acid β-oxidation enzymes. A mitochondrial fatty acid β-oxidation system was thus shown to be present in the retina heterogeneously.

UR - http://www.scopus.com/inward/record.url?scp=78651352218&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78651352218&partnerID=8YFLogxK

U2 - 10.1007/s00418-010-0752-4

DO - 10.1007/s00418-010-0752-4

M3 - Article

C2 - 21046137

AN - SCOPUS:78651352218

VL - 134

SP - 565

EP - 579

JO - Histochemistry and Cell Biology

JF - Histochemistry and Cell Biology

SN - 0948-6143

IS - 6

ER -