Immunohistochemical study of an anti-apoptotic protein survivin in lung cancer

Fumihiko Hoshil, Chiaki Endo, Akira Sakurada, Yuji Matsumura, Yoshinori Okada, Yasushi Hoshikawa, Tetsu Sado, Masafumi Noda, Takashi Kondo

Research output: Contribution to journalArticle

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Abstract

Objective. Survivin is a member of the inhibitor of apoptosis (IAP) family and is considered to play a significant role in the tumorigenesis and proliferation of various kinds of cancers. The purpose of this report is to clarify the relationship between the expression of survivin and prognosis in non-small cell lung cancer (NSCLC). Methods. We examined 100 NSCLC patients who underwent lobectomy in Tohoku University Hospital between October 2000 and December 2004. Expression of survivin and p53 was evaluated by immunohistochemistry. He- siijte The patients consisted of 71 men and 29 women. The average age was 66.1 years old. There were 71 cases of adenocarcinoma and 29 cases of squamous cell carcinoma. Immunohistochemical analyses showed that 64 cases had survivin expression in the cytoplasm, 40 cases had survivin expression in the nucleus, and 36 cases had expression in both the cytoplasm and nucleus. p53-positive staining was detected in 57 cases. There was no significant relationship between expression of survivin and p53. A multivariate analysis with Cox's proportional hazards model showed expression of survivin in the nucleus was an independent poor prognostic factor. Conclusion. Expression of survivin in the nucleus is suggested to be an independent poor prognostic factor in NSCLC. Survivin has several spliced variants and immunohistochemistry can't distinguish among these variants, so immunohistochemistry can't show which variants are important. Further examination with reverse transcription polymerase chain reaction (RT-PCR) is needed to elucidate survivin variants expression.

Original languageEnglish
Pages (from-to)12-16
Number of pages5
JournalJapanese Journal of Lung Cancer
Volume49
Issue number1
DOIs
Publication statusPublished - 01-02-2009

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Apoptosis Regulatory Proteins
Non-Small Cell Lung Carcinoma
Lung Neoplasms
Immunohistochemistry
Cytoplasm
Proportional Hazards Models
Reverse Transcription
Squamous Cell Carcinoma
Carcinogenesis
Adenocarcinoma
Multivariate Analysis
Apoptosis
Staining and Labeling
Polymerase Chain Reaction
Neoplasms

All Science Journal Classification (ASJC) codes

  • Oncology
  • Pulmonary and Respiratory Medicine

Cite this

Hoshil, Fumihiko ; Endo, Chiaki ; Sakurada, Akira ; Matsumura, Yuji ; Okada, Yoshinori ; Hoshikawa, Yasushi ; Sado, Tetsu ; Noda, Masafumi ; Kondo, Takashi. / Immunohistochemical study of an anti-apoptotic protein survivin in lung cancer. In: Japanese Journal of Lung Cancer. 2009 ; Vol. 49, No. 1. pp. 12-16.
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abstract = "Objective. Survivin is a member of the inhibitor of apoptosis (IAP) family and is considered to play a significant role in the tumorigenesis and proliferation of various kinds of cancers. The purpose of this report is to clarify the relationship between the expression of survivin and prognosis in non-small cell lung cancer (NSCLC). Methods. We examined 100 NSCLC patients who underwent lobectomy in Tohoku University Hospital between October 2000 and December 2004. Expression of survivin and p53 was evaluated by immunohistochemistry. He- siijte The patients consisted of 71 men and 29 women. The average age was 66.1 years old. There were 71 cases of adenocarcinoma and 29 cases of squamous cell carcinoma. Immunohistochemical analyses showed that 64 cases had survivin expression in the cytoplasm, 40 cases had survivin expression in the nucleus, and 36 cases had expression in both the cytoplasm and nucleus. p53-positive staining was detected in 57 cases. There was no significant relationship between expression of survivin and p53. A multivariate analysis with Cox's proportional hazards model showed expression of survivin in the nucleus was an independent poor prognostic factor. Conclusion. Expression of survivin in the nucleus is suggested to be an independent poor prognostic factor in NSCLC. Survivin has several spliced variants and immunohistochemistry can't distinguish among these variants, so immunohistochemistry can't show which variants are important. Further examination with reverse transcription polymerase chain reaction (RT-PCR) is needed to elucidate survivin variants expression.",
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Hoshil, F, Endo, C, Sakurada, A, Matsumura, Y, Okada, Y, Hoshikawa, Y, Sado, T, Noda, M & Kondo, T 2009, 'Immunohistochemical study of an anti-apoptotic protein survivin in lung cancer', Japanese Journal of Lung Cancer, vol. 49, no. 1, pp. 12-16. https://doi.org/10.2482/haigan.49.12

Immunohistochemical study of an anti-apoptotic protein survivin in lung cancer. / Hoshil, Fumihiko; Endo, Chiaki; Sakurada, Akira; Matsumura, Yuji; Okada, Yoshinori; Hoshikawa, Yasushi; Sado, Tetsu; Noda, Masafumi; Kondo, Takashi.

In: Japanese Journal of Lung Cancer, Vol. 49, No. 1, 01.02.2009, p. 12-16.

Research output: Contribution to journalArticle

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AU - Hoshil, Fumihiko

AU - Endo, Chiaki

AU - Sakurada, Akira

AU - Matsumura, Yuji

AU - Okada, Yoshinori

AU - Hoshikawa, Yasushi

AU - Sado, Tetsu

AU - Noda, Masafumi

AU - Kondo, Takashi

PY - 2009/2/1

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N2 - Objective. Survivin is a member of the inhibitor of apoptosis (IAP) family and is considered to play a significant role in the tumorigenesis and proliferation of various kinds of cancers. The purpose of this report is to clarify the relationship between the expression of survivin and prognosis in non-small cell lung cancer (NSCLC). Methods. We examined 100 NSCLC patients who underwent lobectomy in Tohoku University Hospital between October 2000 and December 2004. Expression of survivin and p53 was evaluated by immunohistochemistry. He- siijte The patients consisted of 71 men and 29 women. The average age was 66.1 years old. There were 71 cases of adenocarcinoma and 29 cases of squamous cell carcinoma. Immunohistochemical analyses showed that 64 cases had survivin expression in the cytoplasm, 40 cases had survivin expression in the nucleus, and 36 cases had expression in both the cytoplasm and nucleus. p53-positive staining was detected in 57 cases. There was no significant relationship between expression of survivin and p53. A multivariate analysis with Cox's proportional hazards model showed expression of survivin in the nucleus was an independent poor prognostic factor. Conclusion. Expression of survivin in the nucleus is suggested to be an independent poor prognostic factor in NSCLC. Survivin has several spliced variants and immunohistochemistry can't distinguish among these variants, so immunohistochemistry can't show which variants are important. Further examination with reverse transcription polymerase chain reaction (RT-PCR) is needed to elucidate survivin variants expression.

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