Immunoreactivity of glutamate in mouse retina inner segment of photoreceptors with in vivo cryotechnique

Nobuo Terada, Nobuhiko Ohno, Sei Saitoh, Yurika Saitoh, Shinichi Ohno

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

The purpose of this study was to clarify a previously controversial issue concerning glutamate (Glu) immunoreactivity (IR) in the inner segment (IS) of photoreceptors by using in vivo cryotechnique (IVCT) followed by freeze substitution (FS), which enabled us to analyze the cells and tissues reflecting living states. Eyeballs from anesthetized mice were directly frozen using IVCT. The frozen tissues were processed for FS fixation in acetone containing chemical fixatives, and embedded in paraffin. Deparaffinized sections were immunostained with an anti-Glu antibody. The strongest Glu-IR was obtained in the specimens prepared by FS with paraformaldehyde or a low concentration of glutaraldehyde, whereas no Glu-IR was obtained without the chemical fixatives. The Glu was immunolocalized in the IS, outer and inner plexiform and ganglion cell layers. Thus, the immunolocalization of Glu in the IS was clearly demonstrated using IVCT.

Original languageEnglish
Pages (from-to)883-888
Number of pages6
JournalJournal of Histochemistry and Cytochemistry
Volume57
Issue number9
DOIs
Publication statusPublished - 09-2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology

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