TY - JOUR
T1 - Immunoreactivity of VR1 on epidermal keratinocyte of human skin
AU - Denda, Mitsuhiro
AU - Fuziwara, Shigeyoshi
AU - Inoue, Kaori
AU - Denda, Sumiko
AU - Akamatsu, Hirohiko
AU - Tomitaka, Akiko
AU - Matsunaga, Kayoko
PY - 2001
Y1 - 2001
N2 - We demonstrated the immunoreactivity of the receptor proteins, VR1, ion channels associated with pain sensation, on the epidermis of the human skin. Immunohistochemistry using antiserum against VR1 derived peptide showed immunoreactivity on the keratinocytes cell membrane of the human epidermis and cultured keratinocytes. The blocking peptide of the antiserum reduced the immunoreactivity on the epidermis. RT-PCR assay of cultured human keratinocyte also showed expression of VR1 mRNA. These results suggest the existence of VR1-like protein in epidermal keratinocytes of human skin.
AB - We demonstrated the immunoreactivity of the receptor proteins, VR1, ion channels associated with pain sensation, on the epidermis of the human skin. Immunohistochemistry using antiserum against VR1 derived peptide showed immunoreactivity on the keratinocytes cell membrane of the human epidermis and cultured keratinocytes. The blocking peptide of the antiserum reduced the immunoreactivity on the epidermis. RT-PCR assay of cultured human keratinocyte also showed expression of VR1 mRNA. These results suggest the existence of VR1-like protein in epidermal keratinocytes of human skin.
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U2 - 10.1006/bbrc.2001.5299
DO - 10.1006/bbrc.2001.5299
M3 - Article
C2 - 11478791
AN - SCOPUS:0034801364
SN - 0006-291X
VL - 285
SP - 1250
EP - 1252
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 5
ER -