Immunoreactivity of VR1 on epidermal keratinocyte of human skin

Mitsuhiro Denda, Shigeyoshi Fuziwara, Kaori Inoue, Sumiko Denda, Hirohiko Akamatsu, Akiko Yagami, Kayoko Matsunaga

Research output: Contribution to journalArticle

167 Citations (Scopus)

Abstract

We demonstrated the immunoreactivity of the receptor proteins, VR1, ion channels associated with pain sensation, on the epidermis of the human skin. Immunohistochemistry using antiserum against VR1 derived peptide showed immunoreactivity on the keratinocytes cell membrane of the human epidermis and cultured keratinocytes. The blocking peptide of the antiserum reduced the immunoreactivity on the epidermis. RT-PCR assay of cultured human keratinocyte also showed expression of VR1 mRNA. These results suggest the existence of VR1-like protein in epidermal keratinocytes of human skin.

Original languageEnglish
Pages (from-to)1250-1252
Number of pages3
JournalBiochemical and Biophysical Research Communications
Volume285
Issue number5
DOIs
Publication statusPublished - 01-01-2001

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Keratinocytes
Skin
Epidermis
Immune Sera
Peptides
Cell membranes
Ion Channels
Assays
Proteins
Immunohistochemistry
Cell Membrane
Pain
Polymerase Chain Reaction
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Denda, Mitsuhiro ; Fuziwara, Shigeyoshi ; Inoue, Kaori ; Denda, Sumiko ; Akamatsu, Hirohiko ; Yagami, Akiko ; Matsunaga, Kayoko. / Immunoreactivity of VR1 on epidermal keratinocyte of human skin. In: Biochemical and Biophysical Research Communications. 2001 ; Vol. 285, No. 5. pp. 1250-1252.
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Denda, M, Fuziwara, S, Inoue, K, Denda, S, Akamatsu, H, Yagami, A & Matsunaga, K 2001, 'Immunoreactivity of VR1 on epidermal keratinocyte of human skin', Biochemical and Biophysical Research Communications, vol. 285, no. 5, pp. 1250-1252. https://doi.org/10.1006/bbrc.2001.5299

Immunoreactivity of VR1 on epidermal keratinocyte of human skin. / Denda, Mitsuhiro; Fuziwara, Shigeyoshi; Inoue, Kaori; Denda, Sumiko; Akamatsu, Hirohiko; Yagami, Akiko; Matsunaga, Kayoko.

In: Biochemical and Biophysical Research Communications, Vol. 285, No. 5, 01.01.2001, p. 1250-1252.

Research output: Contribution to journalArticle

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