Immunoreactivity of VR1 on epidermal keratinocyte of human skin

Mitsuhiro Denda, Shigeyoshi Fuziwara, Kaori Inoue, Sumiko Denda, Hirohiko Akamatsu, Akiko Tomitaka, Kayoko Matsunaga

Research output: Contribution to journalArticlepeer-review

211 Citations (Scopus)


We demonstrated the immunoreactivity of the receptor proteins, VR1, ion channels associated with pain sensation, on the epidermis of the human skin. Immunohistochemistry using antiserum against VR1 derived peptide showed immunoreactivity on the keratinocytes cell membrane of the human epidermis and cultured keratinocytes. The blocking peptide of the antiserum reduced the immunoreactivity on the epidermis. RT-PCR assay of cultured human keratinocyte also showed expression of VR1 mRNA. These results suggest the existence of VR1-like protein in epidermal keratinocytes of human skin.

Original languageEnglish
Pages (from-to)1250-1252
Number of pages3
JournalBiochemical and Biophysical Research Communications
Issue number5
Publication statusPublished - 2001

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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