Immunoreactivity of VR1 on epidermal keratinocyte of human skin

Mitsuhiro Denda; Shigeyoshi Fuziwara; Kaori Inoue; Sumiko Denda; Hirohiko Akamatsu; Akiko Tomitaka; Kayoko Matsunaga

doi:10.1006/bbrc.2001.5299

Immunoreactivity of VR1 on epidermal keratinocyte of human skin

Mitsuhiro Denda
, Shigeyoshi Fuziwara
, Kaori Inoue
, Sumiko Denda
, Hirohiko Akamatsu
, Akiko Tomitaka
, Kayoko Matsunaga

Research output: Contribution to journal › Article › peer-review

216 Citations (Scopus)

Abstract

We demonstrated the immunoreactivity of the receptor proteins, VR1, ion channels associated with pain sensation, on the epidermis of the human skin. Immunohistochemistry using antiserum against VR1 derived peptide showed immunoreactivity on the keratinocytes cell membrane of the human epidermis and cultured keratinocytes. The blocking peptide of the antiserum reduced the immunoreactivity on the epidermis. RT-PCR assay of cultured human keratinocyte also showed expression of VR1 mRNA. These results suggest the existence of VR1-like protein in epidermal keratinocytes of human skin.

Original language	English
Pages (from-to)	1250-1252
Number of pages	3
Journal	Biochemical and Biophysical Research Communications
Volume	285
Issue number	5
DOIs	https://doi.org/10.1006/bbrc.2001.5299
Publication status	Published - 2001
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1006/bbrc.2001.5299

Cite this

@article{4d171e25243c4b7793913d2be2e108ba,

title = "Immunoreactivity of VR1 on epidermal keratinocyte of human skin",

abstract = "We demonstrated the immunoreactivity of the receptor proteins, VR1, ion channels associated with pain sensation, on the epidermis of the human skin. Immunohistochemistry using antiserum against VR1 derived peptide showed immunoreactivity on the keratinocytes cell membrane of the human epidermis and cultured keratinocytes. The blocking peptide of the antiserum reduced the immunoreactivity on the epidermis. RT-PCR assay of cultured human keratinocyte also showed expression of VR1 mRNA. These results suggest the existence of VR1-like protein in epidermal keratinocytes of human skin.",

author = "Mitsuhiro Denda and Shigeyoshi Fuziwara and Kaori Inoue and Sumiko Denda and Hirohiko Akamatsu and Akiko Tomitaka and Kayoko Matsunaga",

year = "2001",

doi = "10.1006/bbrc.2001.5299",

language = "English",

volume = "285",

pages = "1250--1252",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "5",

}

TY - JOUR

T1 - Immunoreactivity of VR1 on epidermal keratinocyte of human skin

AU - Denda, Mitsuhiro

AU - Fuziwara, Shigeyoshi

AU - Inoue, Kaori

AU - Denda, Sumiko

AU - Akamatsu, Hirohiko

AU - Tomitaka, Akiko

AU - Matsunaga, Kayoko

PY - 2001

Y1 - 2001

N2 - We demonstrated the immunoreactivity of the receptor proteins, VR1, ion channels associated with pain sensation, on the epidermis of the human skin. Immunohistochemistry using antiserum against VR1 derived peptide showed immunoreactivity on the keratinocytes cell membrane of the human epidermis and cultured keratinocytes. The blocking peptide of the antiserum reduced the immunoreactivity on the epidermis. RT-PCR assay of cultured human keratinocyte also showed expression of VR1 mRNA. These results suggest the existence of VR1-like protein in epidermal keratinocytes of human skin.

AB - We demonstrated the immunoreactivity of the receptor proteins, VR1, ion channels associated with pain sensation, on the epidermis of the human skin. Immunohistochemistry using antiserum against VR1 derived peptide showed immunoreactivity on the keratinocytes cell membrane of the human epidermis and cultured keratinocytes. The blocking peptide of the antiserum reduced the immunoreactivity on the epidermis. RT-PCR assay of cultured human keratinocyte also showed expression of VR1 mRNA. These results suggest the existence of VR1-like protein in epidermal keratinocytes of human skin.

UR - https://www.scopus.com/pages/publications/0034801364

UR - https://www.scopus.com/pages/publications/0034801364#tab=citedBy

U2 - 10.1006/bbrc.2001.5299

DO - 10.1006/bbrc.2001.5299

M3 - Article

C2 - 11478791

AN - SCOPUS:0034801364

SN - 0006-291X

VL - 285

SP - 1250

EP - 1252

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 5

ER -

Immunoreactivity of VR1 on epidermal keratinocyte of human skin

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this