IMP-1 type metalo-beta-lactamase producing Serratia marcescens strains isolated from blood culture between 1991 to 2000

Tatsuya Nakamura, Naohiro Shibata, Yohei Doi, Kazuyuki Okuda, Chiyo Nakata, Hitoshi Heijyo, Nobuaki Matsuo, Midori Masuda, Hakuo Takahashi, Yoshichika Arakawa

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Infection caused by metallo beta-lactamase (MBL)-producing bacteria have been increasingly reported in Japan in recent years. We investigated the prevalence, clinical backgrounds and molecular epidemiology of MBL-producing Serratia marcescens strains isolated from blood cultures at our university hospital between 1991 and 2000. Forty-three S. marcescens strains were isolated in the period, and the incidence reached about 2% total bacteria detection in 1998 and 1999. There were 13 ceftazidime-resistant strains (31%), and five of them produced MBL. MICs of imipenem (IPM) were 4-16 mg/ml, of which one strain was susceptible to IPM. Of the five MBL-producers, four were obtained from a tertiary emergency ward, the underlying diseases being either serious trauma or cerebrovascular diseases. The other was isolated from a patient who underwent kidney transplantation. S. marcescens was no longer isolated from patients after administration of aztreonam (AZT), implying that AZT was clinically effective. When analyzed genetically using the AP-PCR technique, the patterns were identical among strains isolated in 1996, 1997 and 1998 and those isolated in 1999 and 2000 respectively, suggesting that the same strains may have inhabited the hospital wards for prolonged periods. Countermeasures against nosocomial infection was undertaken thereafter, resulting in reduction of isolation frequency. It cannot be excluded that the resistant bacteria spread as the amount of carbapenem consumption increased. Prudent use of antibiotics is mandatory to prevent further dissemination of such MBL producers.

Original languageEnglish
Pages (from-to)246-253
Number of pages8
JournalKansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases
Volume76
Issue number4
DOIs
Publication statusPublished - 01-01-2002
Externally publishedYes

Fingerprint

Serratia marcescens
beta-Lactamases
Aztreonam
Imipenem
Bacteria
Cerebrovascular Disorders
Carbapenems
Ceftazidime
Molecular Epidemiology
Cross Infection
Kidney Transplantation
Hospital Emergency Service
Japan
beta-lactamase IMP-1
Blood Culture
Anti-Bacterial Agents
Polymerase Chain Reaction
Incidence
Wounds and Injuries
Infection

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Nakamura, Tatsuya ; Shibata, Naohiro ; Doi, Yohei ; Okuda, Kazuyuki ; Nakata, Chiyo ; Heijyo, Hitoshi ; Matsuo, Nobuaki ; Masuda, Midori ; Takahashi, Hakuo ; Arakawa, Yoshichika. / IMP-1 type metalo-beta-lactamase producing Serratia marcescens strains isolated from blood culture between 1991 to 2000. In: Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases. 2002 ; Vol. 76, No. 4. pp. 246-253.
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IMP-1 type metalo-beta-lactamase producing Serratia marcescens strains isolated from blood culture between 1991 to 2000. / Nakamura, Tatsuya; Shibata, Naohiro; Doi, Yohei; Okuda, Kazuyuki; Nakata, Chiyo; Heijyo, Hitoshi; Matsuo, Nobuaki; Masuda, Midori; Takahashi, Hakuo; Arakawa, Yoshichika.

In: Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases, Vol. 76, No. 4, 01.01.2002, p. 246-253.

Research output: Contribution to journalArticle

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AU - Nakamura, Tatsuya

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