Impact of 2 Gy γ-irradiation on the hallmark characteristics of human bone marrow-derived MSCs

Masaki Iwasa, Sumie Fujii, Aya Fujishiro, Taira Maekawa, Akira Andoh, Akifumi Takaori-Kondo, Tatsuo Ichinohe, Yasuo Miura

Research output: Contribution to journalArticlepeer-review

Abstract

Two gray γ-irradiation is a widely employed basic module for total body irradiation (TBI) in allogeneic hematopoietic cell transplantation (HCT). The effects of γ-irradiation on hematopoietic and immune cells have been well investigated, but its effects on the bone marrow microenvironment (BMM) are unknown. Given the crucial contribution of mesenchymal/stromal stem cells (MSCs) in the BMM to hematopoiesis and osteogenesis, we investigated whether γ-irradiation affects the hallmark characteristics of human bone marrow-derived MSCs (BM-MSCs). Expansion of 2 Gy γ-irradiated BM-MSCs was delayed but eventually recovered. Colony formation and osteogenic, adipogenic, and chondrogenic differentiation capabilities of these cells were extensively suppressed. Irradiation of BM-MSCs did not affect the expansion of CD34 + hematopoietic stem and progenitor cells or production of CD11b + mature myeloid cells in co-cultures. However, it reduced production of CD19 + B-cells, as well as expression of CXCL12 and interleukin-7, which are essential for B-cell lymphopoiesis, in 2 Gy γ-irradiated BM-MSCs. Collectively, colony formation, osteogenic differentiation, and B-cell lymphopoiesis-supportive capabilities of γ-irradiated BM-MSCs were reduced. These effects may predispose survivors receiving HCT with TBI to defective bone formation and a perturbed humoral immune response.

Original languageEnglish
Pages (from-to)703-711
Number of pages9
JournalInternational Journal of Hematology
Volume113
Issue number5
DOIs
Publication statusPublished - 05-2021
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Hematology

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