Impact of catechol-O-methyltransferase (COMT) gene polymorphism on promoter methylation status in gastric mucosa

Tomomitsu Tahara, Tomoyuki Shibata, Tomiyasu Arisawa, Masakatsu Nakamura, Hiromi Yamashita, Daisuke Yoshioka, Masaaki Okubo, Naoko Maruyama, Toshiaki Kamano, Yoshio Kamiya, Hiroshi Fujita, Mitsuo Nagasaka, Masami Iwata, Kazuya Takahama, Makoto Watanabe, Ichiro Hirata

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

DNA methylation is one of the major events in the early process of gastric carcinogenesis and also occurs in non-neoplastic gastric mucosa. Catechol-O-methyltransferase (COMT) catalyzes the methylation of various endobiotic and xenobiotic substances, and protects DNA from oxidative damage. The association between a common functional polymorphism of COMT Val158Met and DNA methylation status in the stomach was investigated. Patients and Methods: One hundred and sixty-nine gastric mucosa samples from non-cancer patients were obtained by endoscopy. The promoter methylation status of p14 and p16 was determined by methylation-specific PCR (MSP). The COMT Val158Met polymorphism was detected by PCR-restriction fragment length polymorphism (RFLP). Results: CpG island methylation was observed in 32.5% of the p14, and 37.9% of the p16. The methylation status of both p14 and p16 was not associated with gender or age, while p16 methylation was strongly associated with Helicobacter pylori infection (OR=4.71, 95% CI=2.35-9.46, p<0.0001). The Val/Val genotype held a significantly higher risk of p16 methylation (OR=3.27, 95% CI=1.05-10.25, p=0.0418). Conclusion: The COMT polymorphism may influence the susceptibility to gene methylation in the gastric mucosa. The promoter CpG island of p16 gene, but not of p14 may be one of the specific regions whose methylation is closely influenced by the COMT polymorphism.

Original languageEnglish
Pages (from-to)2857-2861
Number of pages5
JournalAnticancer research
Volume29
Issue number7
Publication statusPublished - 01-07-2009

Fingerprint

Catechol O-Methyltransferase
Gastric Mucosa
Methylation
Genes
CpG Islands
DNA Methylation
Stomach
p16 Genes
Polymerase Chain Reaction
Helicobacter Infections
Xenobiotics
Helicobacter pylori
Restriction Fragment Length Polymorphisms
Endoscopy
DNA Damage
Carcinogenesis
Genotype

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Tahara, T., Shibata, T., Arisawa, T., Nakamura, M., Yamashita, H., Yoshioka, D., ... Hirata, I. (2009). Impact of catechol-O-methyltransferase (COMT) gene polymorphism on promoter methylation status in gastric mucosa. Anticancer research, 29(7), 2857-2861.
Tahara, Tomomitsu ; Shibata, Tomoyuki ; Arisawa, Tomiyasu ; Nakamura, Masakatsu ; Yamashita, Hiromi ; Yoshioka, Daisuke ; Okubo, Masaaki ; Maruyama, Naoko ; Kamano, Toshiaki ; Kamiya, Yoshio ; Fujita, Hiroshi ; Nagasaka, Mitsuo ; Iwata, Masami ; Takahama, Kazuya ; Watanabe, Makoto ; Hirata, Ichiro. / Impact of catechol-O-methyltransferase (COMT) gene polymorphism on promoter methylation status in gastric mucosa. In: Anticancer research. 2009 ; Vol. 29, No. 7. pp. 2857-2861.
@article{08cb33385462486584f62f21e7da540c,
title = "Impact of catechol-O-methyltransferase (COMT) gene polymorphism on promoter methylation status in gastric mucosa",
abstract = "DNA methylation is one of the major events in the early process of gastric carcinogenesis and also occurs in non-neoplastic gastric mucosa. Catechol-O-methyltransferase (COMT) catalyzes the methylation of various endobiotic and xenobiotic substances, and protects DNA from oxidative damage. The association between a common functional polymorphism of COMT Val158Met and DNA methylation status in the stomach was investigated. Patients and Methods: One hundred and sixty-nine gastric mucosa samples from non-cancer patients were obtained by endoscopy. The promoter methylation status of p14 and p16 was determined by methylation-specific PCR (MSP). The COMT Val158Met polymorphism was detected by PCR-restriction fragment length polymorphism (RFLP). Results: CpG island methylation was observed in 32.5{\%} of the p14, and 37.9{\%} of the p16. The methylation status of both p14 and p16 was not associated with gender or age, while p16 methylation was strongly associated with Helicobacter pylori infection (OR=4.71, 95{\%} CI=2.35-9.46, p<0.0001). The Val/Val genotype held a significantly higher risk of p16 methylation (OR=3.27, 95{\%} CI=1.05-10.25, p=0.0418). Conclusion: The COMT polymorphism may influence the susceptibility to gene methylation in the gastric mucosa. The promoter CpG island of p16 gene, but not of p14 may be one of the specific regions whose methylation is closely influenced by the COMT polymorphism.",
author = "Tomomitsu Tahara and Tomoyuki Shibata and Tomiyasu Arisawa and Masakatsu Nakamura and Hiromi Yamashita and Daisuke Yoshioka and Masaaki Okubo and Naoko Maruyama and Toshiaki Kamano and Yoshio Kamiya and Hiroshi Fujita and Mitsuo Nagasaka and Masami Iwata and Kazuya Takahama and Makoto Watanabe and Ichiro Hirata",
year = "2009",
month = "7",
day = "1",
language = "English",
volume = "29",
pages = "2857--2861",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "7",

}

Tahara, T, Shibata, T, Arisawa, T, Nakamura, M, Yamashita, H, Yoshioka, D, Okubo, M, Maruyama, N, Kamano, T, Kamiya, Y, Fujita, H, Nagasaka, M, Iwata, M, Takahama, K, Watanabe, M & Hirata, I 2009, 'Impact of catechol-O-methyltransferase (COMT) gene polymorphism on promoter methylation status in gastric mucosa', Anticancer research, vol. 29, no. 7, pp. 2857-2861.

Impact of catechol-O-methyltransferase (COMT) gene polymorphism on promoter methylation status in gastric mucosa. / Tahara, Tomomitsu; Shibata, Tomoyuki; Arisawa, Tomiyasu; Nakamura, Masakatsu; Yamashita, Hiromi; Yoshioka, Daisuke; Okubo, Masaaki; Maruyama, Naoko; Kamano, Toshiaki; Kamiya, Yoshio; Fujita, Hiroshi; Nagasaka, Mitsuo; Iwata, Masami; Takahama, Kazuya; Watanabe, Makoto; Hirata, Ichiro.

In: Anticancer research, Vol. 29, No. 7, 01.07.2009, p. 2857-2861.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Impact of catechol-O-methyltransferase (COMT) gene polymorphism on promoter methylation status in gastric mucosa

AU - Tahara, Tomomitsu

AU - Shibata, Tomoyuki

AU - Arisawa, Tomiyasu

AU - Nakamura, Masakatsu

AU - Yamashita, Hiromi

AU - Yoshioka, Daisuke

AU - Okubo, Masaaki

AU - Maruyama, Naoko

AU - Kamano, Toshiaki

AU - Kamiya, Yoshio

AU - Fujita, Hiroshi

AU - Nagasaka, Mitsuo

AU - Iwata, Masami

AU - Takahama, Kazuya

AU - Watanabe, Makoto

AU - Hirata, Ichiro

PY - 2009/7/1

Y1 - 2009/7/1

N2 - DNA methylation is one of the major events in the early process of gastric carcinogenesis and also occurs in non-neoplastic gastric mucosa. Catechol-O-methyltransferase (COMT) catalyzes the methylation of various endobiotic and xenobiotic substances, and protects DNA from oxidative damage. The association between a common functional polymorphism of COMT Val158Met and DNA methylation status in the stomach was investigated. Patients and Methods: One hundred and sixty-nine gastric mucosa samples from non-cancer patients were obtained by endoscopy. The promoter methylation status of p14 and p16 was determined by methylation-specific PCR (MSP). The COMT Val158Met polymorphism was detected by PCR-restriction fragment length polymorphism (RFLP). Results: CpG island methylation was observed in 32.5% of the p14, and 37.9% of the p16. The methylation status of both p14 and p16 was not associated with gender or age, while p16 methylation was strongly associated with Helicobacter pylori infection (OR=4.71, 95% CI=2.35-9.46, p<0.0001). The Val/Val genotype held a significantly higher risk of p16 methylation (OR=3.27, 95% CI=1.05-10.25, p=0.0418). Conclusion: The COMT polymorphism may influence the susceptibility to gene methylation in the gastric mucosa. The promoter CpG island of p16 gene, but not of p14 may be one of the specific regions whose methylation is closely influenced by the COMT polymorphism.

AB - DNA methylation is one of the major events in the early process of gastric carcinogenesis and also occurs in non-neoplastic gastric mucosa. Catechol-O-methyltransferase (COMT) catalyzes the methylation of various endobiotic and xenobiotic substances, and protects DNA from oxidative damage. The association between a common functional polymorphism of COMT Val158Met and DNA methylation status in the stomach was investigated. Patients and Methods: One hundred and sixty-nine gastric mucosa samples from non-cancer patients were obtained by endoscopy. The promoter methylation status of p14 and p16 was determined by methylation-specific PCR (MSP). The COMT Val158Met polymorphism was detected by PCR-restriction fragment length polymorphism (RFLP). Results: CpG island methylation was observed in 32.5% of the p14, and 37.9% of the p16. The methylation status of both p14 and p16 was not associated with gender or age, while p16 methylation was strongly associated with Helicobacter pylori infection (OR=4.71, 95% CI=2.35-9.46, p<0.0001). The Val/Val genotype held a significantly higher risk of p16 methylation (OR=3.27, 95% CI=1.05-10.25, p=0.0418). Conclusion: The COMT polymorphism may influence the susceptibility to gene methylation in the gastric mucosa. The promoter CpG island of p16 gene, but not of p14 may be one of the specific regions whose methylation is closely influenced by the COMT polymorphism.

UR - http://www.scopus.com/inward/record.url?scp=69249163899&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=69249163899&partnerID=8YFLogxK

M3 - Article

C2 - 19596974

AN - SCOPUS:69249163899

VL - 29

SP - 2857

EP - 2861

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 7

ER -

Tahara T, Shibata T, Arisawa T, Nakamura M, Yamashita H, Yoshioka D et al. Impact of catechol-O-methyltransferase (COMT) gene polymorphism on promoter methylation status in gastric mucosa. Anticancer research. 2009 Jul 1;29(7):2857-2861.