In vitro analysis of potency restriction during epiblast differentiation

Mito Kanatsu, Nobuyuki Takakura, Hiroshi Kataoka, Kunihiro Tsuchida, Satomi Nishikawa, Shin Ichi Nishikawa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

During mammalian gastrulation, posterior part of primitive ectoderm differentiates into subsets of mesoderm cells, the most proximal portion of which subsequently gives rise to vascular endothelial cells and hematopoietic cells. To analyze this process in which the potency of multipotent epiblast cells is restricted progressively into hematopoietic cell lineages, we have established a culture system that allows differentiation of epiblast cells as well as embryonic stem cells into hematopoietic cells. Using this culture system, we showed that all parts of epiblast tissues at early streak and late bud stages preserved hematogenic potency, while it was lost from anterior region at early head fold stages. However, this loss of hematogenic potency in the anterior region of the head fold stage embryo was reinduced by addition of activin in the culture. Moreover, in order to detect transitory stages of mesoderm cells that are the direct progeny of multipotent epiblast cells, we developed three monoclonal antibodies that are able to define distinct subsets of mesoderm cells in the gastrulating embryo. These results are discussed from a view of cell-mass based commitment.

Original languageEnglish
Pages (from-to)457-459
Number of pages3
JournalLeukemia
Volume11
Issue numberSUPPL. 3
Publication statusPublished - 01-01-1997

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Germ Layers
Mesoderm
Embryonic Structures
Head
Activins
Gastrulation
Ectoderm
In Vitro Techniques
Cell Lineage
Embryonic Stem Cells
Cell Differentiation
Endothelial Cells
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Kanatsu, M., Takakura, N., Kataoka, H., Tsuchida, K., Nishikawa, S., & Nishikawa, S. I. (1997). In vitro analysis of potency restriction during epiblast differentiation. Leukemia, 11(SUPPL. 3), 457-459.
Kanatsu, Mito ; Takakura, Nobuyuki ; Kataoka, Hiroshi ; Tsuchida, Kunihiro ; Nishikawa, Satomi ; Nishikawa, Shin Ichi. / In vitro analysis of potency restriction during epiblast differentiation. In: Leukemia. 1997 ; Vol. 11, No. SUPPL. 3. pp. 457-459.
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Kanatsu, M, Takakura, N, Kataoka, H, Tsuchida, K, Nishikawa, S & Nishikawa, SI 1997, 'In vitro analysis of potency restriction during epiblast differentiation', Leukemia, vol. 11, no. SUPPL. 3, pp. 457-459.

In vitro analysis of potency restriction during epiblast differentiation. / Kanatsu, Mito; Takakura, Nobuyuki; Kataoka, Hiroshi; Tsuchida, Kunihiro; Nishikawa, Satomi; Nishikawa, Shin Ichi.

In: Leukemia, Vol. 11, No. SUPPL. 3, 01.01.1997, p. 457-459.

Research output: Contribution to journalArticle

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Kanatsu M, Takakura N, Kataoka H, Tsuchida K, Nishikawa S, Nishikawa SI. In vitro analysis of potency restriction during epiblast differentiation. Leukemia. 1997 Jan 1;11(SUPPL. 3):457-459.