In vitro anticancer activity of methyl caffeate isolated from Solanum torvum Swartz. fruit

C. Balachandran, N. Emi, Y. Arun, Y. Yamamoto, B. Ahilan, B. Sangeetha, V. Duraipandiyan, Yoko Inaguma, Akinao Okamoto, S. Ignacimuthu, N. A. Al-Dhabi, P. T. Perumal

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Abstract

The present study was undertaken to investigate the anticancer activity of methyl caffeate isolated from Solanum torvum Swartz. fruit and to explore the molecular mechanisms of action in MCF-7 cells. Cytotoxic properties of hexane, ethyl acetate and methanol extracts were carried out against MCF-7 cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Ethyl acetate extract showed good cytototoxic activities compared to hexane and methanol extracts. Methyl caffeate was isolated from the ethyl acetate extract using column chromatography. Cytotoxic properties of methyl caffeate was investigated against MCF-7, A549, COLO320, HepG-2 and Vero cells. The compound showed potent cytotoxic properties against MCF-7 cells compared to A549, COLO320 and HepG-2 cells. Methyl caffeate significantly reduced cell proliferation and increased formation of fragmented DNA and apoptotic body in MCF-7 cells. Bcl-2, Bax, Bid, p53, caspase-3, PARP and cytochrome c release were detected by western blot analysis. The activities of caspases-3 and PARP gradually increased after the addition of isolated compound. Bcl-2 protein was down regulated; Bid and Bax were up regulated after the treatment with methyl caffeate. Molecular docking studies showed that the compound bound stably to the active sites of poly (ADP-ribose) polymerase-1 (PARP1), B cell CLL/lymphoma-2 (BCL-2), E3 ubiquitin-protein ligase (MDM2) and tubulin. The results strongly suggested that methyl caffeate induced apoptosis in MCF-7 cells via caspase activation through cytochrome c release from mitochondria.

Original languageEnglish
Pages (from-to)81-90
Number of pages10
JournalChemico-Biological Interactions
Volume242
DOIs
Publication statusPublished - 05-12-2015

Fingerprint

Solanum
Fruits
MCF-7 Cells
Fruit
Hexanes
Cytochromes c
Caspase 3
Methanol
Column chromatography
Mitochondria
Vero Cells
Ubiquitin-Protein Ligases
Poly(ADP-ribose) Polymerases
Cell proliferation
B-Cell Lymphoma
Tubulin
Caspases
In Vitro Techniques
methyl caffeate
Chromatography

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Balachandran, C., Emi, N., Arun, Y., Yamamoto, Y., Ahilan, B., Sangeetha, B., ... Perumal, P. T. (2015). In vitro anticancer activity of methyl caffeate isolated from Solanum torvum Swartz. fruit. Chemico-Biological Interactions, 242, 81-90. https://doi.org/10.1016/j.cbi.2015.09.023
Balachandran, C. ; Emi, N. ; Arun, Y. ; Yamamoto, Y. ; Ahilan, B. ; Sangeetha, B. ; Duraipandiyan, V. ; Inaguma, Yoko ; Okamoto, Akinao ; Ignacimuthu, S. ; Al-Dhabi, N. A. ; Perumal, P. T. / In vitro anticancer activity of methyl caffeate isolated from Solanum torvum Swartz. fruit. In: Chemico-Biological Interactions. 2015 ; Vol. 242. pp. 81-90.
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abstract = "The present study was undertaken to investigate the anticancer activity of methyl caffeate isolated from Solanum torvum Swartz. fruit and to explore the molecular mechanisms of action in MCF-7 cells. Cytotoxic properties of hexane, ethyl acetate and methanol extracts were carried out against MCF-7 cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Ethyl acetate extract showed good cytototoxic activities compared to hexane and methanol extracts. Methyl caffeate was isolated from the ethyl acetate extract using column chromatography. Cytotoxic properties of methyl caffeate was investigated against MCF-7, A549, COLO320, HepG-2 and Vero cells. The compound showed potent cytotoxic properties against MCF-7 cells compared to A549, COLO320 and HepG-2 cells. Methyl caffeate significantly reduced cell proliferation and increased formation of fragmented DNA and apoptotic body in MCF-7 cells. Bcl-2, Bax, Bid, p53, caspase-3, PARP and cytochrome c release were detected by western blot analysis. The activities of caspases-3 and PARP gradually increased after the addition of isolated compound. Bcl-2 protein was down regulated; Bid and Bax were up regulated after the treatment with methyl caffeate. Molecular docking studies showed that the compound bound stably to the active sites of poly (ADP-ribose) polymerase-1 (PARP1), B cell CLL/lymphoma-2 (BCL-2), E3 ubiquitin-protein ligase (MDM2) and tubulin. The results strongly suggested that methyl caffeate induced apoptosis in MCF-7 cells via caspase activation through cytochrome c release from mitochondria.",
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Balachandran, C, Emi, N, Arun, Y, Yamamoto, Y, Ahilan, B, Sangeetha, B, Duraipandiyan, V, Inaguma, Y, Okamoto, A, Ignacimuthu, S, Al-Dhabi, NA & Perumal, PT 2015, 'In vitro anticancer activity of methyl caffeate isolated from Solanum torvum Swartz. fruit', Chemico-Biological Interactions, vol. 242, pp. 81-90. https://doi.org/10.1016/j.cbi.2015.09.023

In vitro anticancer activity of methyl caffeate isolated from Solanum torvum Swartz. fruit. / Balachandran, C.; Emi, N.; Arun, Y.; Yamamoto, Y.; Ahilan, B.; Sangeetha, B.; Duraipandiyan, V.; Inaguma, Yoko; Okamoto, Akinao; Ignacimuthu, S.; Al-Dhabi, N. A.; Perumal, P. T.

In: Chemico-Biological Interactions, Vol. 242, 05.12.2015, p. 81-90.

Research output: Contribution to journalArticle

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T1 - In vitro anticancer activity of methyl caffeate isolated from Solanum torvum Swartz. fruit

AU - Balachandran, C.

AU - Emi, N.

AU - Arun, Y.

AU - Yamamoto, Y.

AU - Ahilan, B.

AU - Sangeetha, B.

AU - Duraipandiyan, V.

AU - Inaguma, Yoko

AU - Okamoto, Akinao

AU - Ignacimuthu, S.

AU - Al-Dhabi, N. A.

AU - Perumal, P. T.

PY - 2015/12/5

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N2 - The present study was undertaken to investigate the anticancer activity of methyl caffeate isolated from Solanum torvum Swartz. fruit and to explore the molecular mechanisms of action in MCF-7 cells. Cytotoxic properties of hexane, ethyl acetate and methanol extracts were carried out against MCF-7 cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Ethyl acetate extract showed good cytototoxic activities compared to hexane and methanol extracts. Methyl caffeate was isolated from the ethyl acetate extract using column chromatography. Cytotoxic properties of methyl caffeate was investigated against MCF-7, A549, COLO320, HepG-2 and Vero cells. The compound showed potent cytotoxic properties against MCF-7 cells compared to A549, COLO320 and HepG-2 cells. Methyl caffeate significantly reduced cell proliferation and increased formation of fragmented DNA and apoptotic body in MCF-7 cells. Bcl-2, Bax, Bid, p53, caspase-3, PARP and cytochrome c release were detected by western blot analysis. The activities of caspases-3 and PARP gradually increased after the addition of isolated compound. Bcl-2 protein was down regulated; Bid and Bax were up regulated after the treatment with methyl caffeate. Molecular docking studies showed that the compound bound stably to the active sites of poly (ADP-ribose) polymerase-1 (PARP1), B cell CLL/lymphoma-2 (BCL-2), E3 ubiquitin-protein ligase (MDM2) and tubulin. The results strongly suggested that methyl caffeate induced apoptosis in MCF-7 cells via caspase activation through cytochrome c release from mitochondria.

AB - The present study was undertaken to investigate the anticancer activity of methyl caffeate isolated from Solanum torvum Swartz. fruit and to explore the molecular mechanisms of action in MCF-7 cells. Cytotoxic properties of hexane, ethyl acetate and methanol extracts were carried out against MCF-7 cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Ethyl acetate extract showed good cytototoxic activities compared to hexane and methanol extracts. Methyl caffeate was isolated from the ethyl acetate extract using column chromatography. Cytotoxic properties of methyl caffeate was investigated against MCF-7, A549, COLO320, HepG-2 and Vero cells. The compound showed potent cytotoxic properties against MCF-7 cells compared to A549, COLO320 and HepG-2 cells. Methyl caffeate significantly reduced cell proliferation and increased formation of fragmented DNA and apoptotic body in MCF-7 cells. Bcl-2, Bax, Bid, p53, caspase-3, PARP and cytochrome c release were detected by western blot analysis. The activities of caspases-3 and PARP gradually increased after the addition of isolated compound. Bcl-2 protein was down regulated; Bid and Bax were up regulated after the treatment with methyl caffeate. Molecular docking studies showed that the compound bound stably to the active sites of poly (ADP-ribose) polymerase-1 (PARP1), B cell CLL/lymphoma-2 (BCL-2), E3 ubiquitin-protein ligase (MDM2) and tubulin. The results strongly suggested that methyl caffeate induced apoptosis in MCF-7 cells via caspase activation through cytochrome c release from mitochondria.

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