In Vitro Evolution of Cefiderocol Resistance in an NDMProducing Klebsiella pneumoniae Due to Functional Loss of CirA

Christi L. McElheny, Erin L. Fowler, Alina Iovleva, Ryan K. Shields, Yohei Doi

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

By serially exposing an NDM-producing Klebsiella pneumoniae clinical strain to cefiderocol, we obtained a mutant with cefiderocol MIC of .128 mg/ml. The mutant contained an early stop codon in the iron transporter gene cirA, and its complementation fully restored susceptibility. The cirA-deficient mutant was competed out by the parental strain in vitro, suggesting reduced fitness.

Original languageEnglish
Article numbere01779-21
JournalMicrobiology spectrum
Volume9
Issue number3
DOIs
Publication statusPublished - 12-2021

All Science Journal Classification (ASJC) codes

  • Physiology
  • Ecology
  • Immunology and Microbiology(all)
  • Genetics
  • Microbiology (medical)
  • Cell Biology
  • Infectious Diseases

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