TY - JOUR
T1 - In Vitro Evolution of Cefiderocol Resistance in an NDMProducing Klebsiella pneumoniae Due to Functional Loss of CirA
AU - McElheny, Christi L.
AU - Fowler, Erin L.
AU - Iovleva, Alina
AU - Shields, Ryan K.
AU - Doi, Yohei
N1 - Publisher Copyright:
© 2021 McElheny et al.
PY - 2021/12
Y1 - 2021/12
N2 - By serially exposing an NDM-producing Klebsiella pneumoniae clinical strain to cefiderocol, we obtained a mutant with cefiderocol MIC of .128 mg/ml. The mutant contained an early stop codon in the iron transporter gene cirA, and its complementation fully restored susceptibility. The cirA-deficient mutant was competed out by the parental strain in vitro, suggesting reduced fitness.
AB - By serially exposing an NDM-producing Klebsiella pneumoniae clinical strain to cefiderocol, we obtained a mutant with cefiderocol MIC of .128 mg/ml. The mutant contained an early stop codon in the iron transporter gene cirA, and its complementation fully restored susceptibility. The cirA-deficient mutant was competed out by the parental strain in vitro, suggesting reduced fitness.
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U2 - 10.1128/Spectrum.01779-21
DO - 10.1128/Spectrum.01779-21
M3 - Article
C2 - 34756080
AN - SCOPUS:85122737596
SN - 2165-0497
VL - 9
JO - Microbiology spectrum
JF - Microbiology spectrum
IS - 3
M1 - e01779-21
ER -