In Vitro Evolution of Cefiderocol Resistance in an NDMProducing Klebsiella pneumoniae Due to Functional Loss of CirA

Christi L. McElheny; Erin L. Fowler; Alina Iovleva; Ryan K. Shields; Yohei Doi

doi:10.1128/Spectrum.01779-21

In Vitro Evolution of Cefiderocol Resistance in an NDMProducing Klebsiella pneumoniae Due to Functional Loss of CirA

Christi L. McElheny, Erin L. Fowler, Alina Iovleva, Ryan K. Shields, Yohei Doi

Research output: Contribution to journal › Article › peer-review

52 Citations (Scopus)

Abstract

By serially exposing an NDM-producing Klebsiella pneumoniae clinical strain to cefiderocol, we obtained a mutant with cefiderocol MIC of .128 mg/ml. The mutant contained an early stop codon in the iron transporter gene cirA, and its complementation fully restored susceptibility. The cirA-deficient mutant was competed out by the parental strain in vitro, suggesting reduced fitness.

Original language	English
Article number	e01779-21
Journal	Microbiology spectrum
Volume	9
Issue number	3
DOIs	https://doi.org/10.1128/Spectrum.01779-21
Publication status	Published - 12-2021
Externally published	Yes

All Science Journal Classification (ASJC) codes

Physiology
Ecology
General Immunology and Microbiology
Genetics
Microbiology (medical)
Cell Biology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/Spectrum.01779-21

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title = "In Vitro Evolution of Cefiderocol Resistance in an NDMProducing Klebsiella pneumoniae Due to Functional Loss of CirA",

abstract = "By serially exposing an NDM-producing Klebsiella pneumoniae clinical strain to cefiderocol, we obtained a mutant with cefiderocol MIC of .128 mg/ml. The mutant contained an early stop codon in the iron transporter gene cirA, and its complementation fully restored susceptibility. The cirA-deficient mutant was competed out by the parental strain in vitro, suggesting reduced fitness.",

author = "McElheny, \{Christi L.\} and Fowler, \{Erin L.\} and Alina Iovleva and Shields, \{Ryan K.\} and Yohei Doi",

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year = "2021",

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doi = "10.1128/Spectrum.01779-21",

language = "English",

volume = "9",

journal = "Microbiology spectrum",

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AU - McElheny, Christi L.

AU - Fowler, Erin L.

AU - Iovleva, Alina

AU - Shields, Ryan K.

AU - Doi, Yohei

PY - 2021/12

Y1 - 2021/12

N2 - By serially exposing an NDM-producing Klebsiella pneumoniae clinical strain to cefiderocol, we obtained a mutant with cefiderocol MIC of .128 mg/ml. The mutant contained an early stop codon in the iron transporter gene cirA, and its complementation fully restored susceptibility. The cirA-deficient mutant was competed out by the parental strain in vitro, suggesting reduced fitness.

AB - By serially exposing an NDM-producing Klebsiella pneumoniae clinical strain to cefiderocol, we obtained a mutant with cefiderocol MIC of .128 mg/ml. The mutant contained an early stop codon in the iron transporter gene cirA, and its complementation fully restored susceptibility. The cirA-deficient mutant was competed out by the parental strain in vitro, suggesting reduced fitness.

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U2 - 10.1128/Spectrum.01779-21

DO - 10.1128/Spectrum.01779-21

M3 - Article

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AN - SCOPUS:85122737596

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JF - Microbiology spectrum

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ER -

In Vitro Evolution of Cefiderocol Resistance in an NDMProducing Klebsiella pneumoniae Due to Functional Loss of CirA

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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