In Vitro Evolution of Cefiderocol Resistance in an NDMProducing Klebsiella pneumoniae Due to Functional Loss of CirA

Christi L. McElheny; Erin L. Fowler; Alina Iovleva; Ryan K. Shields; Yohei Doi

doi:10.1128/Spectrum.01779-21

In Vitro Evolution of Cefiderocol Resistance in an NDMProducing Klebsiella pneumoniae Due to Functional Loss of CirA

Christi L. McElheny
, Erin L. Fowler
, Alina Iovleva
, Ryan K. Shields
, Yohei Doi

Research output: Contribution to journal › Article › peer-review

56 Citations (Scopus)

Abstract

By serially exposing an NDM-producing Klebsiella pneumoniae clinical strain to cefiderocol, we obtained a mutant with cefiderocol MIC of .128 mg/ml. The mutant contained an early stop codon in the iron transporter gene cirA, and its complementation fully restored susceptibility. The cirA-deficient mutant was competed out by the parental strain in vitro, suggesting reduced fitness.

Original language	English
Article number	e01779-21
Journal	Microbiology spectrum
Volume	9
Issue number	3
DOIs	https://doi.org/10.1128/Spectrum.01779-21
Publication status	Published - 12-2021
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Physiology
Ecology
General Immunology and Microbiology
Genetics
Microbiology (medical)
Cell Biology
Infectious Diseases

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10.1128/Spectrum.01779-21

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title = "In Vitro Evolution of Cefiderocol Resistance in an NDMProducing Klebsiella pneumoniae Due to Functional Loss of CirA",

abstract = "By serially exposing an NDM-producing Klebsiella pneumoniae clinical strain to cefiderocol, we obtained a mutant with cefiderocol MIC of .128 mg/ml. The mutant contained an early stop codon in the iron transporter gene cirA, and its complementation fully restored susceptibility. The cirA-deficient mutant was competed out by the parental strain in vitro, suggesting reduced fitness.",

author = "McElheny, \{Christi L.\} and Fowler, \{Erin L.\} and Alina Iovleva and Shields, \{Ryan K.\} and Yohei Doi",

note = "Publisher Copyright: {\textcopyright} 2021 McElheny et al.",

year = "2021",

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doi = "10.1128/Spectrum.01779-21",

language = "English",

volume = "9",

journal = "Microbiology spectrum",

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AU - McElheny, Christi L.

AU - Fowler, Erin L.

AU - Iovleva, Alina

AU - Shields, Ryan K.

AU - Doi, Yohei

PY - 2021/12

Y1 - 2021/12

N2 - By serially exposing an NDM-producing Klebsiella pneumoniae clinical strain to cefiderocol, we obtained a mutant with cefiderocol MIC of .128 mg/ml. The mutant contained an early stop codon in the iron transporter gene cirA, and its complementation fully restored susceptibility. The cirA-deficient mutant was competed out by the parental strain in vitro, suggesting reduced fitness.

AB - By serially exposing an NDM-producing Klebsiella pneumoniae clinical strain to cefiderocol, we obtained a mutant with cefiderocol MIC of .128 mg/ml. The mutant contained an early stop codon in the iron transporter gene cirA, and its complementation fully restored susceptibility. The cirA-deficient mutant was competed out by the parental strain in vitro, suggesting reduced fitness.

UR - https://www.scopus.com/pages/publications/85122737596

UR - https://www.scopus.com/pages/publications/85122737596#tab=citedBy

U2 - 10.1128/Spectrum.01779-21

DO - 10.1128/Spectrum.01779-21

M3 - Article

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AN - SCOPUS:85122737596

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In Vitro Evolution of Cefiderocol Resistance in an NDMProducing Klebsiella pneumoniae Due to Functional Loss of CirA

Abstract

UN SDGs

All Science Journal Classification (ASJC) codes

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