TY - JOUR
T1 - In vitro generation of lymphohematopoietic cells from endothelial cells purified from murine embryos
AU - Nishikawa, Shin Ichi
AU - Nishikawa, Satomi
AU - Kawamoto, Hiroshi
AU - Yoshida, Hisahiro
AU - Kizumoto, Masami
AU - Kataoka, Hiroshi
AU - Katsura, Yoshimoto
N1 - Funding Information:
We thank N. Matsuyoshi for VECD1 mAb and L. Zon and S. Fraser for critical reading of the manuscript. This study is supported by grants from the Ministry of Education, Science, Sports, and Culture of Japan (No. 07CE2005, 06277102, 06NP1101).
PY - 1998/6
Y1 - 1998/6
N2 - We have investigated the lymphohematopoietic potentials of endothelial cells (EC) and hematopoietic cells (HPC) sorted from embryos. Expression of VE-cadherin, CD45, and Ter119 was used to distinguish EC (VE- cadherin+CD45-Ter119-) from HPC (VE-cadherin-CD45+). Thus defined, EC population takes up acetylated LDL and coexpresses CD31, Flk1, and CD34. In E9.5 embryos, EC from yolk sac (YS) and the embryo proper generate blood cells, including lymphocytes. Thus, lymphohematopoietic EC do exist in the embryo, and they are generated both in YS and the embryo proper. On the other hand, HPC with lymphopoietic potency appear first in the embryo proper. These findings implicate involvement of multiple environmental cues for acquiring lymphopoietic competency during differentiation of HPC.
AB - We have investigated the lymphohematopoietic potentials of endothelial cells (EC) and hematopoietic cells (HPC) sorted from embryos. Expression of VE-cadherin, CD45, and Ter119 was used to distinguish EC (VE- cadherin+CD45-Ter119-) from HPC (VE-cadherin-CD45+). Thus defined, EC population takes up acetylated LDL and coexpresses CD31, Flk1, and CD34. In E9.5 embryos, EC from yolk sac (YS) and the embryo proper generate blood cells, including lymphocytes. Thus, lymphohematopoietic EC do exist in the embryo, and they are generated both in YS and the embryo proper. On the other hand, HPC with lymphopoietic potency appear first in the embryo proper. These findings implicate involvement of multiple environmental cues for acquiring lymphopoietic competency during differentiation of HPC.
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U2 - 10.1016/S1074-7613(00)80581-6
DO - 10.1016/S1074-7613(00)80581-6
M3 - Article
C2 - 9655490
AN - SCOPUS:17744416048
SN - 1074-7613
VL - 8
SP - 761
EP - 769
JO - Immunity
JF - Immunity
IS - 6
ER -
