In vitro generation of lymphohematopoietic cells from endothelial cells purified from murine embryos

Shin Ichi Nishikawa, Satomi Nishikawa, Hiroshi Kawamoto, Hisahiro Yoshida, Masami Kizumoto, Hiroshi Kataoka, Yoshimoto Katsura

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292 Citations (Scopus)

Abstract

We have investigated the lymphohematopoietic potentials of endothelial cells (EC) and hematopoietic cells (HPC) sorted from embryos. Expression of VE-cadherin, CD45, and Ter119 was used to distinguish EC (VE- cadherin+CD45-Ter119-) from HPC (VE-cadherin-CD45+). Thus defined, EC population takes up acetylated LDL and coexpresses CD31, Flk1, and CD34. In E9.5 embryos, EC from yolk sac (YS) and the embryo proper generate blood cells, including lymphocytes. Thus, lymphohematopoietic EC do exist in the embryo, and they are generated both in YS and the embryo proper. On the other hand, HPC with lymphopoietic potency appear first in the embryo proper. These findings implicate involvement of multiple environmental cues for acquiring lymphopoietic competency during differentiation of HPC.

Original languageEnglish
Pages (from-to)761-769
Number of pages9
JournalImmunity
Volume8
Issue number6
DOIs
Publication statusPublished - 06-1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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